Integrative recombination of Lactobacillus delbrueckii bacteriophage mv4: Functional analysis of the reaction and structure of the attP site

Molecular and General Genetics

Volumn 262, Issue 2, 1999, Pages 355-366

  Auvray, F ^a   Coddeville, M ^a   Espagno, G ^a   Ritzenthaler, P ^a  

^a Lab de Microbiologie et de G   (France)

Author keywords

attP site; Bacteriophage; Integrase; Lactobacillus; Site specific recombination

Indexed keywords

DNA FRAGMENT; INTEGRASE;

ARTICLE; BACTERIOPHAGE; ENZYME ACTIVITY; ENZYME BINDING; ENZYME PURIFICATION; ESCHERICHIA COLI; GENE DELETION; GENETIC RECOMBINATION; GRAM POSITIVE BACTERIUM; LACTOBACILLUS DELBRUECKII; LYSOGENIZATION; NONHUMAN; NUCLEOTIDE SEQUENCE; PLASMID; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS;

BACTERIOPHAGES; BASE SEQUENCE; ESCHERICHIA COLI; HISTIDINE; INTEGRASES; LACTOBACILLUS; MOLECULAR SEQUENCE DATA; RECOMBINATION, GENETIC; VIRUS INTEGRATION;

EID: 0032846763     PISSN: 00268925     EISSN: None     Source Type: Journal    
DOI: 10.1007/s004380051094     Document Type: Article

References (42)

1. Abremski K, Hoess R (1992) Evidence for a second conserved arginine residue in the integrase family of recombination proteins. Protein Eng 5:87-91
2. Argos P, Landy A, Abremski K, Egan JB, Haggard-Ljungquist E, Hess RH, Kahn ML, Kalionis B, Narayana SVL, Pierson III LS, Sternberg N, Leong JM (1986) The integrase family of site-specific recombinases: regional similarities and global diversity. EMBO J 5:433-440
3. Auvray F, Coddeville M, Ritzenthaler P, Dupont L (1997) Plasmid integration in a wide range of bacteria mediated by the integrase of Lactobacillus delbrueckii bacteriophage mv4. J Bacteriol 179:1837-1845
4. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248-254
5. Campbell A (1992) Chromosomal insertion sites for phages and plasmids. J Bacteriol 174:7495-7499
6. Craig NL, Nash HA (1984) E. coli integration host factor binds to specific sites in DNA. Cell 39:707-716
7. Dupont L, Boizet-Bonhoure B, Coddeville M, Auvray F, Ritzenthaler P (1995) Characterization of genetic elements required for site-specific integration of Lactobacillus delbrueckii subsp. bulgaricus bacteriophage mv4 and construction of an integration-proficient vector for Lactobacillus plantarum. J Bacteriol 177:586-595
8. Echols H (1970) Integrative and excisive recombination by bacteriophage lambda: evidence for an excision-specific recombination. J Mol Biol 47:575-583
9. Esposito D, Scocca J (1997) The integrase family of tyrosine recombinases: evolution of a conserved active site domain. Nucleic Acids Res 25:3605-3614
10. Gardner JF, Nash HA (1986) Role of Escherichia coli IHF protein in lambda site-specific recombination. J Mol Biol 191:181-189
11. Goodman SD, Scocca J (1989) Nucleotide sequence and expression of the gene for the site-specific integration protein from bacteriophage HP1 of Haemophilus influenzae. J Bacteriol 171:4232-4240
12. Guarneros G, Echols H (1973) Thermal asymmetry of site-specific recombination in bacteriophage lambda. Virology 52:30-38
13. Hakimi JM, Scocca JJ (1996) Purification and characterization of the integrase from the Haemophilus influenzae bacteriophage HP1: identification of a four-strand intermediate and the order of strand exchange. Mol Microbiol 21:147-158
14. Hauser M, Scocca J (1992) Site-specific integration of the Haemophilus influenzae bacteriophage HP1: location of the boundaries of the phage attachment site. J Bacteriol 174:6674-6677
15. Hsu PL, Ross W, Landy A (1980) The lambda phage att site: functional limits and interaction with Int protein. Nature 285:85-91
16. Kim S, Landy A (1992) Lambda Int protein bridges between higher order complexes at two distant chromosomal loci attL and attR. Science 256:198-203
17. Kim S, Moitoso de Vargas L, Nunes-Düby SE, Landy A (1990) Mapping of a higher order protein-DNA complex: two kinds of long-range interactions in lambda attL. Cell 63:773-781
18. Kolot M, Gottfried P, Yagil E (1996) A second site-specific recombination event in the lambdoid bacteriophage HK022. Mol Gen Genet 253:362-369
19. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685
20. Landy A (1989) Dynamic, structural, and regulatory aspects of lambda site-specific recombination. Annu Rev Biochem 58:913-949
21. Le Bourgeois P, Lautier M, Mata M, Ritzenthaler P (1992) New tools for the physical and genetic mapping of Lactococcus strains. Gene 111:109-114
22. Lee MH, Hatfull GF (1993) Mycobacteriophage L5 integrase-mediated site-specific integration in vitro. J Bacteriol 175:6836-6841
23. Lillehaug D, Birkeland NK (1993) Characterization of genetic elements required for site-specific integration of the temperate bacteriophage φLC3 and construction of integration-negative φ LC3 mutants. J Bacteriol 175:1745-1755
24. Mayer MP (1995) A new set of useful cloning and expression vectors derived from pBlueScript. Gene 163:41-46
25. Mizuuchi M, Mizuuchi K (1980) Integrative recombination of bacteriophage lambda: extent of the DNA sequence involved in attachment site function. Proc Natl Acad Sci USA 77:3220-3224
26. Mizuuchi M, Mizuuchi K (1985) The extent of DNA sequence required for a functional bacterial attachment site of phage lambda. Nucleic Acids Res 13:1193-1208
27. Moitoso de Vargas L, Pargellis CA, Hasan NM, Bushman EW, Landy A (1988) Autonomous DNA binding domains of lambda integrase recognize two different sequence families. Cell 54:923-929
28. Moitoso de Vargas L, Kim S, Landy A (1989) DNA looping generated by DNA bending protein IHF and the two domains of lambda integrase. Science 244:1457-1461
29. Nash H, Robertson CA (1981) Purification and properties of the Escherichia coli protein factor required for lambda integrative recombination. J Biol Chem 256:9246-9253
30. Nunes-Düby SE, Kwon HJ, Tirumalai RS, Ellenberg T, Landy A (1998) Similarities and differences among 105 members of the Int family of site-specific recombinases. Nucleic Acids Res 26:391-406
31. Pedulla ML, Lee MH, Lever DC, Hatfull GF (1996) A novel host factor for integration of mycobacteriophage L5. Proc Natl Acad Sci USA 93:15411-15416
32. Pena CE, Lee MH, Pedulla ML, Hatfull GF (1997) Characterization of the mycobacteriophage L5 attachment site, attP. J Mol Biol 266:76-92
33. Poyart-Salmeron C, Trieu-Cuot P, Carlier C, Courvalin P (1989) Molecular characterization of two proteins involved in the excision of the pneumococcal transposon Tn1545: homologies with other site-specific recombinases. EMBO J 8:2425-2433
34. Reiter WD, Palm P, Yeats S (1989) Transfer RNA genes frequently serve as integration sites for prokaryotic genetic elements. Nucleic Acids Res 17:1907-1914
35. Richet E, Abcarian P, Nash H (1988) Synapsis of attachment sites during lambda integrative recombination involves capture of a naked DNA by a protein-DNA complex. Cell 52:9-17
36. Ross W, Landy A (1982) Bacteriophage lambda int protein recognizes two classes of sequence in the phage att site: characterization of arm-type sites. Proc Natl Acad Sci USA 79:7724-7728
37. Ross W, Landy A (1983) Patterns of lambda Int recognition in the regions of strand exchange. Cell 33:261-272
38. Sadowski P (1993) Site-specific genetic recombination: hops, flips, and flops. FASEB J. 7:760-767
39. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual (2nd edn). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
40. Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74:5463-5467
41. Smith-Mungo L, Chan IT, Landy A (1994) Structure of the P22 att site. Conservation and divergence in the lambda motif of recombinogenic complexes. J Biol Chem 269:20798-20805
42. Yu A, Haggard-Ljungquist E (1993) Characterization of the binding sites of two proteins involved in the bacteriophage P2 site-specific recombination system. J Bacteriol 175:1239-1249