Generation of full-length cDNA library from single human prostate cancer cells

BioTechniques

Volumn 27, Issue 3, 1999, Pages 410-414

  Ying, Shao Yao ^a   Lui, Hannah M ^a   Lin, Shi Lung ^a   Chuong, Cheng Ming ^a  

^a UNIVERSITY OF SOUTHERN CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; CANCER CELL; GENE AMPLIFICATION; GENE LIBRARY; HUMAN; HUMAN CELL; POLYMERASE CHAIN REACTION; PROSTATE CANCER;

EID: 0032845547     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/99273bm02     Document Type: Article

References (6)

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2. Eberwine, J., H. Yeh, K. Miyashiro, Y. Cao, S. Nair, R. Finnell, M. Zettel and P. Coleman. 1992. Analysis of gene expression in single live neurons. Proc. Natl. Acad. Sci. USA 89:3010-3014.
3. Embleton, M.J., G. Gorochov, P.T. Jones and G. Winter. 1992. In-cell PCR from mRNA: amplifying and linking the rearranged immunoglobulin heavy and light chain V-genes within single cells. Nucleic Acids Res. 20:3831-3837.
4. O'Dell, D.M., P. Raghupathi, P.B. Crino, B. Morrison III, J.H. Eberwine and T.K. McIntosh. 1998. Amplification of mRNAs from single, fixed, TUNEL-positive cells. BioTechniques 25:566-570.
5. Patanjali, S.R., S. Parimoo and S.M. Weissman. 1991. Construction of a uniform abundance (normalized) cDNA library. Proc. Natl. Acad. Sci. USA 88:1943-1947.
6. Sambrook, J., E.F. Fritsch and T. Maniatis. 1989. Construction and analysis of cDNA libraries, p. 8.11-8.35. In Molecular Cloning: A Laboratory Manual, 2nd ed. CSH Laboratory Press, Cold Spring Harbor, NY.