A tumour necrosis factor-alpha (TNF-α) sensitive adherent KYM-1D4 derived cell line: Use in TNF-α cytotoxicity assays in the presence of actinomycin D

Journal of Immunological Methods

Volumn 227, Issue 1-2, 1999, Pages 197-198

  Meager, Anthony ^a  

^a NATIONAL INSTITUTE FOR BIOLOGICAL STANDARDS AND CONTROL   (United Kingdom)

Author keywords

Adherent; Human rhabdomyosarcoma cell line; Tumour necrosis factor, Bioassay

Indexed keywords

3 (4,5 DIMETHYL 2 THIAZOLYL) 2,5 DIPHENYLTETRAZOLIUM BROMIDE; DACTINOMYCIN; PHENAZINE METHOSULFATE; TETRAZOLE DERIVATIVE; TUMOR NECROSIS FACTOR ALPHA;

ANIMAL CELL; ARTICLE; ASSAY; CELL ADHESION; CELL CULTURE; CONTROLLED STUDY; CULTURE MEDIUM; CYTOTOXICITY; FIBROBLAST; HUMAN; HUMAN CELL; NONHUMAN; PRIORITY JOURNAL; RHABDOMYOSARCOMA; SARCOMA CELL;

CELL SURVIVAL; DACTINOMYCIN; DOSE-RESPONSE RELATIONSHIP, DRUG; HUMANS; TUMOR CELLS, CULTURED; TUMOR NECROSIS FACTOR-ALPHA;

ANIMALIA;

EID: 0032839966     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-1759(99)00081-2     Document Type: Article

References (5)

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2. Meager A., Gaines-Das R.E. International collaborative study of the candidate international standards for human tumour necrosis factors alpha (hTNF-α) and beta (hTNF-β) and for murine tumour necrosis factor alpha (mTNF-α). J. Immunol. Methods. 170:1994;1.
3. Meager A., Leung H., Woolley J. Assays for tumour necrosis factor and related cytokines. J. Immunol. Methods. 116:1987;1.
4. Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J. Immunol. Methods. 65:1983;55.
5. Sekiguchi M., Shiroko Y., Suzuki T., Imada M., Miyahara M., Fujii G. Characterisation of a human rhabdomyosarcoma cell strain in tissue culture. Biomed. Pharmacother. 39:1985;372.