Heterogeneity in glutamic acid decarboxylase expression among single rat pancreatic beta cells

Diabetologia

Volumn 42, Issue 9, 1999, Pages 1086-1092

  Sanchez Soto M C ^a   Larrieta, M E ^a   Vidaltamayo, R ^a   Hiriart, M ^a  

^a INSTITUTO DE FISIOLOGÍA CELULAR   (Mexico)

Author keywords

GABA; GAD65; GAD67; Insulin secretion; Pancreatic beta cells; Reverse hemolytic plaque assay

Indexed keywords

BUCLADESINE; GLUCOSE; GLUTAMATE DECARBOXYLASE; INSULIN; NERVE GROWTH FACTOR;

ANIMAL CELL; ARTICLE; CONTROLLED STUDY; INSULIN RELEASE; NONHUMAN; PANCREAS ISLET BETA CELL; PRIORITY JOURNAL; PROTEIN EXPRESSION; RAT;

ANIMALS; CELLS, CULTURED; GLUCOSE; GLUTAMATE DECARBOXYLASE; ISLETS OF LANGERHANS; ISOENZYMES; MALE; MICROSCOPY, CONFOCAL; RATS; RATS, WISTAR; TIME FACTORS;

EID: 0032839150     PISSN: 0012186X     EISSN: None     Source Type: Journal    
DOI: 10.1007/s001250051275     Document Type: Article

References (31)

1. Sorenson RL Garry DG, Brelje, TC (1991) Structural and functional considerations of GABA in islets of Langerhans. Diabetes 40: 1365-1374
2. Karlsen AE, Petersen JS, Hagopian WA et al. (1994) Islet cell expression and autoimmunity to two isoforms of glutamic acid decarboxylase in relation to insulin-dependent diabetes. In:PR Flatt, Lenzen S (eds) Insulin secretion and pancreatic B-cell research. Smith-Gordon, UK, pp 499-507
3. Erlander MG, Tillakaratne NJ, Feldblum S, Ratel N, Tobin AJ (1991) Two genes encode distinct glutamate decarboxylases. Neuron 7: 91-100
4. Christagau S, Schierbeck H, Aanstoot HJ et al. (1991) Pancreatic B-cells express two forms of glutamic acid decarboxylase, a 65-kDa hydrophilic form and a 64-kDa amphiphilic form which can be both membrane-bound and soluble. J Biol Chem 266: 21257-21264
5. Baekkeskov S, Aanstoot HJ, Christagau S et al. (1990) Identification of a 64 K autoantigen in insulin-dependent diabetes as the GABA-synthesizing enzyme glutamic acid decarboxylase. Nature 347: 151-156
6. Solimena M, De Camilli P (1991) Autoimmunity to glutamic acid decarboxylase (GAD) in Stiff-Man syndrome and insulin-dependent diabetes mellitus. Trends Neurosci 14: 452-457
7. Jaeger C, Allendörfer J, Hatziagelaki E et al. (1997) Persistent GAD 65 antibodies in longstanding IDDM are not associated with residual beta-cell function, neuropathy or HLA-DR status. Horm Metab Res 29: 510-515
8. Gaskins RH, Baldeón ME, Selassie L, Beverly JL (1995) Glucose modulates γ-aminobutyric acid release from pancreatic βTC6. J Biol Chem 270: 30286-30289
9. Smismans A, Schuit F, Pipeleers D (1997) Nutrient regulation of gamma-aminobutyric acid release from islet beta cells. Diabetologia 40:1411-1415
10. Katoh J, Taniguchi H, Kasuga M (1995) Response of glutamic acid decarboxylase to glucose but not arginine in islets. Life Sci 21: 1799-1805
11. Hao W, Li L, Mehta V, Lernmark A, Palmer J (1994) Functional state of the cell affects expression of both forms of glutamic acid decarboxylase. Pancreas 9: 558-562
12. Pipeleers D (1987) The biosociology of pancreatic β-cells. Diabetologia 30: 277-291
13. Hiriart M, Ramirez-Medeles MC (1991) Functional subpopulations of individual pancreatic B-cells in culture. Endocrinology 128: 3193-3198
14. Van Schravendijk CFH, Kikiens R, Pipeleers D (1992) Pancreatic β cell heterogeneity in glucose-induced insulin secretion. J Biol Chem 267: 21344-21348
15. Heimberg H, De Vos A, Vandercammen A et al. (1993) Heterogeneity in glucose sensitivity among pancreatic βcells is correlated to differences in glucose phosphorylation rather than glucose transport. EMBO J 12: 2873-2879
16. Hiriart M, Ramirez-Medeles MC (1993) Muscarinic modulation of insulin secretion by single pancreatic B-cells. Mol Cell Endocrinol 93: 63-69
17. Hiriart M, Sánchez Soto C, Ramírez-Medeles MC, Malaisse W (1995) Functional heterogeneity of single pancreatic beta cells stimulated by L-leucine and the methyl ester of succinic or glutamic acid. Biochem Mol Med 54: 133-137
18. Pipeleers D, Ling Z (1992) Pancreatic beta cells in insulin-dependent diabetes. Diabetes Metab Rev 8: 209-227
19. Vidaltamayo R, Sánchez-Soto C, Rosenbaum T et al. (1996) Neuron-like phenotypic changes in pancreatic beta cells induced by NGF, FGF and dbcAMP. Endocrine 4: 19-26
20. Frödin M, Sekine N, Roche E et al. (1995) Glucose, other secretagogues, and nerve growth factor stimulate mitogen-activated protein kinase in the insulin-secreting β cell line INS-1. J Biol Chem 270:7882-7889
21. Rosenbaum T, Vidaltamayo R, Sánchez-Soto C, Zentella A, Hiriart M (1998) Pancreatic β cells synthesize and secrete nerve growth factor. Proc Natl Acad Sci USA 95: 7784-7788
22. Kaufman DL, Houser CR, Tobin AJ (1991) Two forms of the γ-aminobutyric acid synthetic enzyme glutamate decarboxylase have distinct intraneuronal distributions and cofactor interactions. J Neurochem 56: 720-723
23. Chang YC, Gottlieb DI (1988) Characterization of the proteins purified with monoclonal antibodies to glutamic acid decarboxylase. J Neurosci 8: 2123-2130
24. Neill JD, Frawley LS (1983) Detection of hormone release from individual cells in mixed populations using a reverse hemolytic plaque assay. Endocrinology 112:1135-1137
25. Hiriart M, Matteson DR (1988) Na channels and two types of Ca channels in rat pancreatic B-cells identified with the reverse hemolytic plaque assay. J Gen Physiol 91: 617-639
26. Jorns A (1994) Immunocytochemical and ultrastructural heterogeneities of normal and glibenclamide stimulated pancreatic beta cells in the rat. Virchows Arch 425: 305-313
27. Faulker-Jones B, Cram DS, Kun J, Harrison LC (1993) Localization and quantitation of expression of two glutamate decarboxylase genes in pancreatic beta cells and other peripheral tissues of mouse and rat. Endocrinology 133: 2962-2972
28. Bjork E, Kampe O, Karlsson FA et al. (1992) Glucose regulation of the autoantigen GAD65 in human pancreatic islets. J Clin Endocrinol Metab 75: 1574-1576
29. Petersen JS, Russel S, Marshal MO et al. (1993) Differential expression of glutamic acid decarboxylase in rat and human islets. Diabetes 42: 484-495
30. Degli Espositi M, Mackay IR (1997) The GABA network and the pathogenesis of IDDM. Diabetologia 40: 352-356
31. Hiriart M, Martínez-Merlos MT, Ramírez-Medeles MC (1997) Physiological behaviour of functional subpopulations of single pancreatic β cells. Adv Exp Med Biol 426: 267-274