Cloning and purification of bacteriophage K11 RNA polymerase

BioTechniques

Volumn 27, Issue 4, 1999, Pages 690-694

  Rong, Minqing ^a   Castagna, Ray ^a   McAllister, William T ^a  

^a SUNY DOWNSTATE MEDICAL CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

RNA POLYMERASE;

ARTICLE; BACTERIOPHAGE; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME PURIFICATION; ENZYME SPECIFICITY; GENE EXPRESSION SYSTEM; GENETIC TRANSCRIPTION; MOLECULAR CLONING; NONHUMAN; PROMOTER REGION; PROTEIN EXPRESSION;

EID: 0032837670     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/99274bm13     Document Type: Article

References (26)

1. Arnaud, N., V. Cheynet, G. Oriol, B. Mandrand and F. Mallet. 1997. Construction and expression of a modular gene encoding bacteriophage T7 RNA polymerase. Gene 199:149-156.
2. Davanloo, P., A.H. Rosenberg, J.J. Dunn and F.W. Studier. 1984. Cloning and expression of the gene for bacteriophage T7 RNA polymerase. Proc. Natl. Acad. Sci. USA 81:2035-2039.
3. Dietz, A. 1985. Untersuchungen zur evolution von verwandten des phagen T7 durch vergleichende DNA-basensequenzanalyse. Doctoral Thesis, University of Freiburg, Freiburg, Germany.
4. Dietz, A., H.J. Weisser, H. Kossel and R. Hausmann. 1990. The gene for Klebsiella bacteriophage K11 RNA polymerase: sequence and comparison with the homologous genes of phages T7, T3, and SP6. Mol. Gen. Genet. 221:283-286.
5. Durbin, R.K. 1999. Gene expression systems based on bacteriophage T7 RNA polymerase, p. 10-45. In J.M. Fernandez and J.P. Hoeffler (Eds.), Gene Expression Systems - Using Nature for the Art of Expression. Academic Press, San Diego.
6. Ellinger, T. and R. Ehricht, 1998. Single-step purification of T7 RNA polymerase with a 6-histidine tag. BioTechniques 24:718-720.
7. Fuerst, T.R., E.G. Niles, F.W. Studier and B. Moss. 1986. Eukaryotic transient-expression system based on recombinant vaccinia virus that synthesizes bacteriophage T7 RNA polymerase. Proc. Nat. Acad. Sci. USA 83:8122-8126.
8. Guatelli, J.C., K.M. Whitfield, D.Y. Kwoh, K.J. Barringer, D.D. Richman and T.R. Gingeras. 1990. Isothermal, in vitro amplification of nucleic acids by a multienzyme reaction modeled after retroviral replication. Proc. Nat. Acad. Sci. USA 87:1874-1878.
9. Hausmann, R. 1988. The T7 group, p. 259-289. In R. Calendar (Ed.), The Bacteriophages. Plenum Press, New York.
10. He, B., M. Kong, D.L. Lyakhov, H. Gartenstein, G.A. Diaz, R.C. Castagna, W.T. McAllister and R.K. Durbin. 1997. Rapid generation and purification of mutant phage RNA polymerases. Protein Expr. Purif. 9:142-151.
11. Jorgensen, E.D., R.K. Durbin, S.S. Risman and W.T. McAllister. 1991. Specific contacts between the bacteriophage T3, T7, and SP6 RNA polymerases and their promoters. J. Biol. Chem. 266:645-651.
12. Kotani, H., Y. Ishizaki, N. Hiraoka and A. Obayashi. 1987. Nucleotide sequence and expression of the cloned gene of bacteriophage SP6 RNA polymerase. Nucleic Acids Res. 15:2653-2664.
13. McAllister, W.T. 1993. Structure and function of the bacteriophage T7 RNA polymerase (or, the virtues of simplicity). Cell Mol. Biol. Res. 59:385-391.
14. McAllister, W.T. 1997. Transcription by T7 RNA polymerase, p. 15-25. In F. Eckstein and D. Lilley (Eds.), Nucleic Acids and Molecular Biology. Springer, Berlin.
15. Milligan, J.F. and O.C. Uhlenbeck. 1989. Synthesis of small RNAs using T7 RNA polymerase. Methods Enzymol. 180:51-62.
16. Molineux, I.J. 1999. Bacteriophage T7 and related bacteriophages, p. 1388-1396. In R.G. Webster and A. Granoff (Eds.), Encyclopedia of Virology. Academic Press, New York.
17. Morris, C.E., J.F. Klement and W.T. McAllister. 1986. Cloning and expression of the bacteriophage T3 RNA polymerase gene. Gene 41:193-200.
18. Raskin, C.A., G.A. Diaz, K. Joho and W.T. McAllister. 1992. Substitution of a single bacteriophage T3 residue in bacteriophage T7 RNA polymerase at position 748 results in a switch in promoter specificity. J. Mol. Biol. 228:506-515.
19. Rong, M. 1998. Characterization of promoter specificity determinants and template requirements of T7 RNA polymerase. Doctoral Thesis, State University of New York, Brooklyn, NY.
20. Rong, M., B. He, W.T. McAllister and R.K. Durbin. 1998. Promoter specificity determinants of T7 RNA polymerase. Proc. Nat. Acad. Sci. USA 95:515-519.
21. Sambrook, J., E.F. Fritsch and T. Maniatis. 1989. Molecular Cloning: A Laboratory Manual, 2nd ed., CHS Laboratory Press, Cold Spring Harbor, NY.
22. Studier, F.W. 1979. Relationships among different strains of T7 and among T7-related bacteriophages. Virology 95:70-84.
23. Studier, F.W., A.H. Rosenberg, J.J. Dunn and J.W. Dubendorff. 1990. Use of T7 RNA polymerase to direct expression of cloned genes. Methods Enzymol. 185:60-89.
24. Tabor, S. and C.C. Richardson. 1985. A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes. Proc. Nat. Acad. Sci. USA 82:1074-1078.
25. Temiakov, D., P.E. Karasavas and W.T. McAllister. Characterization of T7 RNA polymerase protein:DNA interactions during the initiation and elongation phases. In A.A. Travers and M. Buckle (Eds.), Protein:DNA Interactions: A Practical Approach. Oxford University Press, Oxford (In press).
26. Zhou, Y., T.J. Giordano, R.K. Durbin and W.T. McAllister. 1990. Synthesis of functional mRNA in mammalian cells by bacteriophage T3 RNA polymerase. Mol. Cell Biol. 10:4529-4537.