Purification and characterization of 5-oxo-L-prolinase from Paecilomyces varioti F-1, an ATP-dependent hydrolase active with L-2-oxothiazolidine-4- carboxylic acid

Archives of Microbiology

Volumn 172, Issue 3, 1999, Pages 182-185

  Mochizuki, Kazuya ^a  

^a NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY AIST   (Japan)

Author keywords

5 Oxo L prolinase; 5 Oxo L proline; L 2 Oxothiazolidine 4 carboxylic acid; Paecilomyces varioti F 1

Indexed keywords

2 OXO 4 THIAZOLIDINECARBOXYLIC ACID; ADENOSINE TRIPHOSPHATE; CARBOXYLIC ACID; HYDROLASE; PROLYL DIPEPTIDASE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE DEHYDROGENASE;

ANIMAL EXPERIMENT; ARTICLE; ENANTIOMER; ENZYME ACTIVATION; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME ISOLATION; ENZYME PURIFICATION; FUNGUS; MOUSE; NONHUMAN; PAECILOMYCES VARIOTI; PH; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL;

AMIDOHYDROLASES; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME INHIBITORS; HYDROGEN-ION CONCENTRATION; PAECILOMYCES; PYRROLIDONECARBOXYLIC ACID; SULFHYDRYL REAGENTS; THIAZOLES; THIAZOLIDINES;

EID: 0032837287     PISSN: 03028933     EISSN: None     Source Type: Journal    
DOI: 10.1007/s002030050758     Document Type: Article

References (12)

1. Beales D, Finch R, McLean AEM, Smith M, Wilson ID (1981) Determination of penicillamine and other thiols by combined high-performance liquid chromatography and post-column reaction with Ellman's reagent: Application to human urine. J Chromatogr 226: 498-503
2. Fujiwara M, Ishida Y, Nimura N, Toyama A, Kinoshita T (1987) Postcolumn fluorometric detection system for liquid chromatographic analysis of amino and imino acids using o-phthalalde-hyde/N-acetyl-L-cysteine reagent. Anal Biochem 166:72-78
3. Gilman JC (1957) A Manual of Soil Fungi, 2nd Edn. The Iowa State College Press, Ames, Iowa
4. Habeeb AFSA (1972) Reaction of protein sulfhydryl groups with Ellman's reagent. In: Hirs CHW, Timasheff SN (eds) Methods in Enzymology, vol 25. Academic Press, New York, pp 457-464
5. Koyama H (1988) Purification and characterization of 5-oxo-L-prolinase (L-pyroglutamate hydrolase) from Alcaligenes sp. F-137. Agric Biol Chem 52:735-741
6. Mochizuki K, Yamazaki Y, Hosono K (1990) Preparation of optically pure (S)-(+)-2-oxothiazoline-4-carboxylic acid from a racemate by microbial degradation of the (R)-enantiomer. Agric Biol Chem 54:543-544
7. Mochizuki K, Hosono K, Kobayashi H (1994) Purification and some properties of a novel racemase, which racemizes 2-oxo-thiazolidine-4-carboxylic acid and 5-oxoproline, from Flecto-bacillus sp. strain B-1. Biochim Biophys Acta 1200:27-33
8. Raper KB, Thom C (1949) A Manual of the Penicillia. Williams & Wilkins, Baltimore
9. Seddon AP, Li L, Meister A (1985) 5-Oxo-L-prolinase from Pseudomonas putida. In: Meister A (ed) Methods in Enzymology, vol 113. Academic Press, New York, pp 451-458
10. Van Der Werf P, Meister A (1974) Isolation of 5-oxoprolinase from a prokaryote. Biochem Biophys Res Commun 56:90-96
11. Williamson JM, Meister A (1981) Stimulation of hepatic glutathione formation by administration of L-2-oxothiazolidine-4-carboxylic acid, a 5-oxo-L-prolinase substrate. Proc Natl Acad Sci USA 78:936-939
12. Williamson JM, Meister A (1982) New substrate of 5-oxo-L-prolinase. J Biol Chem 257:12039-12042