Ultrastructural localization of Hsp-72 examined with a new polyclonal antibody raised against the truncated variable domain of the heat shock protein

Cell Stress and Chaperones

Volumn 4, Issue 2, 1999, Pages 139-152

  Kurucz, Istrán ^a   Tombor, Bálint ^a   Prechl, József ^c   Erdö, Franciska ^b   Hegedüs, Erzsébet ^a   Nagy, Zoltán ^a   Vitai, Marta ^a   Korányi, László ^a   László, Lajos ^c  

^a Dept of Cell and Molecular Biology ^*  (Hungary)

^b POB 348 H 8201   (Hungary)

^c EÖTVÖS LORÁND UNIVERSITY   (Hungary)

Author keywords

[No Author keywords available]

Indexed keywords

HEAT SHOCK PROTEIN 72; POLYCLONAL ANTIBODY;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; BINDING AFFINITY; COATED PIT; CONTROLLED STUDY; ELECTRON MICROSCOPY; ENDOSOME; HUMAN; HUMAN CELL; IMMUNOCHEMISTRY; IMMUNOGOLD STAINING; LYSOSOME; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN LOCALIZATION; PROTEIN STRUCTURE; PROTEIN VARIANT; RAT; ROUGH ENDOPLASMIC RETICULUM;

ANIMALIA;

EID: 0032837150     PISSN: 13558145     EISSN: None     Source Type: Journal    
DOI: 10.1379/1466-1268(1999)004<0139:ULOHEW>2.3.CO;2     Document Type: Article

References (74)

1. Barbe MF, Tytell M, Gower DJ and Welch WJ (1988) Hyperthermia protects against light damage in the rat retina. Science 241: 1817-1820.
2. Beck SC and De Maio A (1994) Stabilization of protein synthesis in thermotolerant cells during heat shock. J Biol Chem 269: 21803-21811.
3. Beck SC, Paidas CN, Mooney ML, Deitch EA and De Maio A (1995) Presence of the stress-inducible form of hsp-70 (hsp-72) in normal rat colon. Shock 3: 398-402.
4. Beckmann RP, Mizzen LA and Welch WJ (1990) Interaction of hsp70 with newly synthesized proteins: implications for protein folding and assembly. Science 248: 850-854.
5. Bizzi A, Schaetzle B, Patton A, Gambetti P and Autilio-Gambetti L (1991) Axonal transport of two major components of the ubiquitin system: free ubiquitin and ubiquitin carboxyl-terminal hydrolase PGP 9.5. Brain Res 548: 292-299.
6. Blom DJ De Waard-Siebinga I, Apte RS, Luyten GP, Niederkorn JV and Jager MJ (1997) Effrect of hyperthermia on expression of histocompatibility antigens and heat-shock protein molecules on three human ocular melanoma cell lines. Melanoma Res 7: 103-109.
7. Blond-Elguindi S, Cwirla SE, Dower WJ, Lipshutz RJ, Sprang SR, Sambrook JF and Gething MJ (1993) Affinity panning of a library of peptides displayed on bacteriophages reveals the binding specificity of BiP. Cell 75: 717-728.
8. Camins A, Diez-Fernandez C, Camarasa J and Escubedo E (1995) Cell surface expression of heat shock proteins in dog neutrophils induced by mitochondrial bezodiazepine receptor ligands. Immunopharmacology 29: 159-166.
9. Chomczynski P and Sacchi N (1987) Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 162: 156-159.
10. Craig EA, Gambill BD and Nelson RJ (1993) Heat shock proteins: molecular chaperones of protein biogenesis. Microbiol Rev 57: 402-414.
11. Cuervo AM, Dice JF and Knecht E (1997) A population of rat liver lysosomes responsible for the selective uptake and degradation of cytosolic protein. J Biol Chem 272: 5606-5615.
12. Dhillon VB, McCallum S, Norton P, Twomey BM, Erkeller-Yuksel F, Lydyard P, Isenberg DA and Latchman DS (1993) Differential heat shock protein overexpression and its clinical relevance in systemic lupus erythematosus. Ann Rheum Dis 52: 436-442.
13. DiCesare S, Poccia F, Mastino A and Colizzi V (1992) Surface expressed heat-shock proteins by stressed or human immunodeficiency virus (HIV)-infected lymphoid cells represent the target for antibody-dependent cellular cytotoxicity. Immunology 76: 341-343.
14. Dice FJ (1990) Peptide sequences that target cytosolic proteins for lysosomal proteolysis. TIBS 15: 305-309.
15. Edwards DP, Estes PA, Fadok VA, Bona BJ, Onate S, Nordeen SK and Welch WJ (1992) Heat shock alters the composition of heteromeric steroid receptor complexes and enhances receptor activity in vivo. Biochemistry 31: 2482-2491.
16. Favatier F, Bornman L, Hightower LE, Gunther E and Polla BS (1997) Variation in hsp gene expression and Hsp polymorphism: do they contribute to differential disease susceptibility and stress tolerance? Cell Stress & Chaperones 2: 141-155.
17. Ferrarini M, Heltai S, Zicchi MR and Rugarli C (1992) Unusual expression and localization of heat-shock proteins in human tumor cells. Int J Cancer 51: 613-619.
18. Getchell TV, Krishna NS, Dhooper N, Sparks DL and Getchell ML (1995) Human olfactory receptor neurons express heat shock protein 70: age-related trends. Ann Otol Rhinol Laryngol 104: 47-56.
19. Gething MJ and Sambrook J (1992) Protein folding in the cell. Nature 355: 33-45.
20. Guzhova IV, Arnholdt AC, Darieva ZA, Kinev AV, Lasunskia EB, Nilsson K, Bozhkov VM, Voronin AP and Margulis BA (1998) Effect of exogenous stress protein 70 on the functional properties of human promonocytes through binding to cell surface and internalization. Cell Stress & Chaperones 3: 67-77.
21. Hang H and Fox M (1996) Levels of 70 kDa heat shock protein through the cell cycle in several mammalian cell lines. Cytometry 25: 367-373.
22. Hattori H, Kaneda T, Lokeshwar B, Laszlo A and Ohtsuka K (1993) A stress-inducible 40 kDa protein (hsp-40): purification by modified two-dimensional gel electrophoresis and colocalization with hsc70 (p73) in heat-shocked HeLa cells. J Cell Sci 104: 629-638.
23. Hernandes-Pando R, Pedrasa-Chaverri J, Orozco-Estevez H, Silva-Serna P, Moreno I, Rondan-Zarate A, Elonos M, Correa-Rotter R and Larriva-Sahd J (1995) Histological and subcellular distribution of 65 and 70 kD heat shock proteins in experimental nephrotoxic injury. Exp Toxic Pathol 47: 501-508.
24. Heufelder AE, Wenzel BE and Bahn RS (1992) Cell surface localization of a 72 kilodalton heat shock protein in retroocular fibroblasts from patients with Grave' ophtalmopathy. J Clin Endocrinol Metab 74: 732-736.
25. Hicks GR and Raikhel NV (1995) Protein import into the nucleus: an integrated view. Annu Rev Cell Dev Biol 11: 155-188.
26. Imamoto N, Matsuoka Y, Kurihara T, Kohno K, Miyagi M, Sakiyama F, Okada Y, Tsunasawa S and Yoneda Y (1992) Antibodies against 70-kD heat shock cognate protein inhibit mediated nuclear import of karyophilic proteins. J Cell Biol 119: 1047-1061.
27. Jaattela M, Wissing D, Bauer PA and Li GC (1992) Major heat shock protein HSP70 protects tumor cells from tumor necrosis factor cytotoxicity. EMBO J 11: 3507-1352.
28. Kurucz I, Jost CR, George AJT, Andrew SM and Segal D (1993) A bacterially expressed single-chain Fv construct from the 2B4 T-cell receptor. Proc Natl Acad Sci USA 90: 3830-3834.
29. Laemmli UK (1970) Cleavage of the structural proteins during the assembly of the head of bacteriophage T4. Nature 277: 680-685.
30. Lamian V, Small GM and Feldherr CM (1996) Evidence for the existence of a novel mechanism for the nuclear import of Hsc-70. Exp Cell Res 228: 84-91.
31. László L, Tuckwell J, Self T, Lowe J, Landon M, Smith S, Hawthorne JN and Mayer, R.J. (1991) The latent membrane protein-1 in Epstein-Barr virustransformed lymhoblastoid cells is found with ubiquitin-protein conjugates and heat-shock protein 70 in lysosomes oriented around the microtubule organising centre. J Pathology 164: 203-214.
32. László L, Lowe J, Self T, Kenvard N, Landon M, McBride T, Farquhar C, McConnell I., Brown J, Hope J and Mayer RJ (1992) Lysosomes as key organelles in the pathogenesis of prion encephalopathies. J Pathology 166: 333-341.
33. Latruffe N and Vamecq J (1997) Peroxisome proliferators and peroxisome proliferator activated receptors (PPARs) as regulators of lipid metabolism. Biochimie 79: 81-94.
34. Leung S-M, Senisterra G, Ritchie KP, Sadis SE, Lepock JR and Hightower LE (1996) Thermal activation of the bovine Hsc70 molecular chaperone at physiological temperatures: physical evidence of a molecular thermometer. Cell Stress & Chaperones 1: 78-89.
35. Liu Y, Liang S and Tartakoff AM (1996) Heat shock disassembles the nucleolus and inhibits nuclear protein import and poly(A)+ RNA export. EMBO J 15: 6750-6757
36. Mach H, Middaugh CR and Lewis RV (1992) Statistical determination of the average values of the extinction coefficients of tryptophan and tyrosine in native proteins. Anal Biochem 200: 74-80.
37. Mandell RB and Feldherr CM (1990) Identification of two Hsp-70 related Xenopus oocyte proteins that are capable of recycling across the nuclear envelope. J Cell Biol 11: 1775-1783.
38. Marchuk D, Drumm M, Saulino A and Collins FS (1991) Construction of T-vectors, a rapid and general system for direct cloning of unmodified PCR products. Nucl Acids Res 19: 1154.
39. Matthew A, Bell A and Johnstone RM (1995) Hsp-70 is closely associated with the transferrin receptor in exosomes from maturing reticulocytes. Biochem J 308: 823-830.
40. Matts RL, Xu Z, Pal JK and Chen JJ (1992) Interaction of the Hemeregulated eIF-2a kinase with heat shock proteins in rabbit reticulocyte lysate. J Biol Chem 267: 18160-18167
41. Mehta HB, Popovich BK, Dillmann WH (1988) Ischemia induces changes in the level of mRNAs coding for stress protein 71 and creatine kinase M. Circ Res 63: 512-517.
42. Merrick BA, He C, Witcher LL, Patterson RM, Reid JJ, Pence-Pawlowski PM and Selkirk JK (1996) HSP binding and mitochondrial localization of p53 protein in human HT1080 and mouse C3H10T1/2 cell lines. Biochim Biophys Acta 1297: 57-68.
43. Mestril R, Chi SH, Sayen MR, Dillmann WH (1994a) Isolation of a novel inducible rat heat-shock protein (HSP70) gene and its expression during ischaemia/hypoxia and heat shock. Biochem J 298: 561-569.
44. Mestril R, Chi SH, Sayen MR, O'Reilly K and Dillmann WH (1994b) Expression of inducible stress protein 70 in rat heart myogenic cells confers protection against simulated ischemia-induced injury. J Clin Invest 93: 759-767.
45. Multhoff G, Botzler C, Wiesnet M, Muller E, Meier T and Wilmanns W (1995) A stress-inducible 72 kDa heat-shock protein (Hsp-72) is expressed on the surface of human tumor cells but not on normal cells. Int J Cancer 61: 272-279.
46. Nagy Z, Rajnavolgyi E, Hollosi M, Toth GK, Varadi G, Penke B, Toth I, Horvath A, Gergely J and Kurucz I (1994) The intersubunit region of the influenza virus haemagglutinin is recognized by antibodies during infection. Scand J Immunol 40: 281-291.
47. Nihei T, Sato N, Takahashi S, Ishikawa M, Sagae S, Kudo R, Kikuchi K and Inoue A (1993) Demonstration of selective protein complexes of p53 with 73 kDa heat shock cognate protein, but not with 72 kDa heat shock protein in human tumor cells. Cancer Letters 73: 181-189.
48. Nossner E, Goldberg JE, Naftzger C, Lyu SC, Clayberger C and Krensky AM (1996) HLA-derived peptides which inhibit T-cell function bind to members of the heat-shock protein 70 family. J Exp Med 183: 339-348.
49. Nowak TS (1991) Localization of 70 kDa stress protein mRNA induction in gerbil brain after ischemia. J Cerebral Blood Flow Metab 11: 432-439.
50. Oglesbee MJ, Liu Z, Kenney H and Brooks CL (1996) The highly inducible member of the 70 kDa family of heat shock proteins increases canine distemper virus polymerase activity. J Gen Virol 77: 2125-2135.
51. Ohtsuka K, Tanabe K, Nakamura H and Sato C (1986) Possible cytoskeletal association of 69,000-and 68,000-Dalton heat shock proteins and structural relations among heat shock proteins in murine mastocytoma cells. Radiation Research 108: 34-42.
52. Okuno Y, Imamoto N and Yoneda Y (1993) 70-kDa heat-shock cognate protein colocalizes with karyophilic proteins into the nucleus during their transport in vitro. Exp Cell Res 206: 134-142.
53. Poccia F, Piselli P, Vendetti S, Bach S, Amendola A, Placido R and Colizzi V (1996) Heat-shock protein expression on the membrane of T cells undergoing apoptosis. Immunology 88: 6-12.
54. Polla BS, Kantengwa S, Francois D, Salvioli S, Franceschi C, Marsac C and Cossarizza A (1996) Mitochondria are selective targets for the protective effects of heat shock against oxidative injury. Proc Natl Acad Sci USA 93: 6458-6463.
55. Protti MP, Heltai S, Bellone M, Ferrarini M, Manfredi AA and Rugarli C (1994) Constitutive expression of the heat shock protein 72 kDa in human melanome cells. Cancer Lett 85: 211-216.
56. Rassow J, Voos W and Pfanner N (1995) Partner proteins determine multiple functions of Hsp70. Trends Cell Biol 5: 207-212.
57. Rattner JB (1991) Hsp-70 is localized to the centrosome of dividing cells. Exp Cell Res 195: 110-113.
58. Rothman JE and Schmid SL (1986) Enzymatic recycling of clathrin from coated vesicles. Cell 46: 5-9.
59. Rubin GM and Hogness DS (1975) Effect od heat shock on the synthesis of low molecular weight RNAs in Drosophila accumulation of a novel form of 5S RNA. Cell 6: 207-213.
60. Sambrook J, Fritsch EF and Maniatis T (eds) Molecular cloning, a laboratory manual. Cold Spring Harbor Laboratory Press, 1989
61. Schatz G and Dobberstein B (1993) Common principles of protein translocation across membranes. Science 271: 1519-1526.
62. Shi Y and Thomas JO (1992) The transport of proteins into the nucleus requires the 70-kilodalton heat shock protein or its cytosolic cognate. Molec Cellular Biol 12: 2186-2192.
63. Shulga N Roberts P, Gu Z, Spitz L, Tabb MM, Nomura M and Goldfarb DS (1996) In vivo nuclear transport kinetics in Saccharomyces cerevisiae: a role for heat shock protein 70 during targeting and translocation. J Cell Biol 135: 329-339.
64. Sorger PK and Pelham HR (1987) Cloning and expression of a gene encoding hsc-73, the major hsp70-like protein in unstressed rat cells. EMBO J 6: 993-998.
65. Studier FW, Rosenberg AH, Dunn JJ and Dubendorff JW (1990) Use of T7 RNA polymerase to direct expression of cloned genes. Methods Enzymol 185: 60-89.
66. Terlecky SR (1994) Hsp70s and lysosomal proteolysis. Experientia 50: 1021-1025.
67. Vanbuskirk A, Crump BL, Margoliash E and Pierce SK (1989) A peptide binding protein having a role in antigen presentation is a member of the Hsp-70 heat shock family. J Exp Med 170: 1799-1809.
68. Vass K, Welch WJ and Nowak TS (1988) Localization of 70-kDa stress protein induction in gerbil brain after ischemia. Acta Neuropathol 77: 128-135.
69. Wang T-F, Chang J-h and Wang C (1993) Identification of the peptide binding domain of hsc70. J Biol Chem 268: 26049-26051.
70. Welch WJ, Feramisco JR (1982) Purification of the major mammalian heat shock proteins. J Biol Chem 257: 14949-14959.
71. Welch WJ and Feramisco JR (1984) Nuclear and nucleolar localization of the 72,00-dalton heat shock protein in heat-shocked mammalian cells. J Biol Chem 259: 4501-4513.
72. Welch WJ and Suhan JP (1985) Morphological study of the mammalian stress response: Characterization of the changes in cytoplasmic organelles, cytoskeleton, and nucleoli, and appearance of intranuclear actin filaments in rat fibroblasts after heat-shock treatment. J Cell Biol 101: 1198-1211.
73. Welch WJ (1992) Mammalian stress response:cell phisilogy, structure/function of stress proteins and implications for medicine and disease. Physiol Rev 72: 1063-1081.
74. Yang J and DeFranco DB (1994) Differential roles of heat shock protein 70 in the in vitro nuclear import of glucocorticoid receptor and simian virus 40 large tumor antigen. Mol Cell Biol 14: 5088-5098.