-
1
-
-
0031030057
-
Processive degradation of proteins and other catalytic properties of the proteasome from Thermoplasma acidophilum
-
Akopian, T. N., A. F. Kisselev, and A. L. Goldberg. 1997. Processive degradation of proteins and other catalytic properties of the proteasome from Thermoplasma acidophilum. J. Biol. Chem. 272:1791-1798.
-
(1997)
J. Biol. Chem.
, vol.272
, pp. 1791-1798
-
-
Akopian, T.N.1
Kisselev, A.F.2
Goldberg, A.L.3
-
2
-
-
0025183708
-
Basic local alignment search tool
-
Altschul, S. F., W. Gish, W. Miller, E. W. Myers, and D. J. Lipman. 1990. Basic local alignment search tool. J. Mol. Biol. 215:403-410.
-
(1990)
J. Mol. Biol.
, vol.215
, pp. 403-410
-
-
Altschul, S.F.1
Gish, W.2
Miller, W.3
Myers, E.W.4
Lipman, D.J.5
-
3
-
-
0345523687
-
The reddening of salted hides and fish
-
Anderson, H. 1954. The reddening of salted hides and fish. Appl. Microbiol. 2:64-69.
-
(1954)
Appl. Microbiol.
, vol.2
, pp. 64-69
-
-
Anderson, H.1
-
4
-
-
0033168717
-
Eukaryotic 20S proteasome catalytic subunit propeptides prevent active site inactivation by N-terminal acetylation and promote particle assembly
-
Arendt, C. S., and M. Hochstrasser. 1999. Eukaryotic 20S proteasome catalytic subunit propeptides prevent active site inactivation by N-terminal acetylation and promote particle assembly. EMBO J. 18:3575-3585.
-
(1999)
EMBO J.
, vol.18
, pp. 3575-3585
-
-
Arendt, C.S.1
Hochstrasser, M.2
-
5
-
-
0003174184
-
Preparation and analysis of DNA
-
Green Publishing Associates and Wiley-Interscience, New York, N.Y
-
Ausubel, F. M., R. Brent, R. E. Kingston, D. D. Moore, J. A. Smith, J. G. Sideman, and K. Struhl. 1987. Preparation and analysis of DNA, p. 2.0.1-2.9.10. In Current protocols in molecular biology. Green Publishing Associates and Wiley-Interscience, New York, N.Y.
-
(1987)
Current Protocols in Molecular Biology
, pp. 201-2910
-
-
Ausubel, F.M.1
Brent, R.2
Kingston, R.E.3
Moore, D.D.4
Smith, J.A.5
Sideman, J.G.6
Struhl, K.7
-
6
-
-
0031038205
-
Purification and characterization of a proteasome from the hyperthermophilic archaeon Pyrococcus furiosus
-
Bauer, M. W., S. B. Halio, and R. M. Kelly. 1997. Purification and characterization of a proteasome from the hyperthermophilic archaeon Pyrococcus furiosus. Appl. Environ. Microbiol. 63:1160-1164.
-
(1997)
Appl. Environ. Microbiol.
, vol.63
, pp. 1160-1164
-
-
Bauer, M.W.1
Halio, S.B.2
Kelly, R.M.3
-
7
-
-
0032488846
-
The proteasome: Paradigm of a self-compartmentalizing protease
-
Baumeister, W., J. Walz, F. Zühl, and E. Seemüller. 1998. The proteasome: paradigm of a self-compartmentalizing protease. Cell 92:367-380.
-
(1998)
Cell
, vol.92
, pp. 367-380
-
-
Baumeister, W.1
Walz, J.2
Zühl, F.3
Seemüller, E.4
-
8
-
-
0017184389
-
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
-
Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:248-254.
-
(1976)
Anal. Biochem.
, vol.72
, pp. 248-254
-
-
Bradford, M.M.1
-
9
-
-
0028972449
-
A protein catalytic framework with an N-terminal nucleophile is capable of self-activation
-
Brannigan, J. A., G. Dodson, H. J. Duggleby, P. C. E. Moody, J. L. Smith, D. R. Tomchick, and A. G. Murzin. 1995. A protein catalytic framework with an N-terminal nucleophile is capable of self-activation. Nature 378:416-419.
-
(1995)
Nature
, vol.378
, pp. 416-419
-
-
Brannigan, J.A.1
Dodson, G.2
Duggleby, H.J.3
Moody, P.C.E.4
Smith, J.L.5
Tomchick, D.R.6
Murzin, A.G.7
-
10
-
-
16044367245
-
Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii
-
Bult, C. J., O. White, G. J. Olsen, L. Zhou, D. Fleischmann, G. G. Sutton, J. A. Blake, M. FitzGerald, R. A. Clayton, J. D. Gocayne, A. R. Kerlavage, B. A. Dougherty, J.-F. Tomb, M. D. Adams, C. I. Reich, R. Overbeek, E. F. Kirkness, K. G. Weinstock, J. M. Merrick, A. Glodek, J. L. Scott, N. S. M. Geoghagen, J. F. Weidman, J. L. Fuhrmann, D. Nguyen, T. R. Utterback, J. M. Kelley, J. D. Peterson, P. W. Sadow, M. C. Hanna, M. D. Cotton, K. M. Roberts, M. A. Hurst, B. P. Kaine, M. Borodovsky, H.-P. Klenk, C. M. Fraser, H. O. Smith, C. R. Woese, and J. C. Venter. 1996. Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii. Science 273:1058-1073.
-
(1996)
Science
, vol.273
, pp. 1058-1073
-
-
Bult, C.J.1
White, O.2
Olsen, G.J.3
Zhou, L.4
Fleischmann, D.5
Sutton, G.G.6
Blake, J.A.7
Fitzgerald, M.8
Clayton, R.A.9
Gocayne, J.D.10
Kerlavage, A.R.11
Dougherty, B.A.12
Tomb, J.-F.13
Adams, M.D.14
Reich, C.I.15
Overbeek, R.16
Kirkness, E.F.17
Weinstock, K.G.18
Merrick, J.M.19
Glodek, A.20
Scott, J.L.21
Geoghagen, N.S.M.22
Weidman, J.F.23
Fuhrmann, J.L.24
Nguyen, D.25
Utterback, T.R.26
Kelley, J.M.27
Peterson, J.D.28
Sadow, P.W.29
Hanna, M.C.30
Cotton, M.D.31
Roberts, K.M.32
Hurst, M.A.33
Kaine, B.P.34
Borodovsky, M.35
Klenk, H.-P.36
Fraser, C.M.37
Smith, H.O.38
Woese, C.R.39
Venter, J.C.40
more..
-
11
-
-
0028907938
-
Genes encoded in the major histocompatibility complex affecting the generation of peptides for TAP transport
-
Cerundolo, V., A. Kelly, T. Elliott, J. Trowsdale, and A. Townsend. 1995. Genes encoded in the major histocompatibility complex affecting the generation of peptides for TAP transport. Eur. J. Immunol. 25:554-562.
-
(1995)
Eur. J. Immunol.
, vol.25
, pp. 554-562
-
-
Cerundolo, V.1
Kelly, A.2
Elliott, T.3
Trowsdale, J.4
Townsend, A.5
-
12
-
-
0024525020
-
Genome mapping in halohacteria
-
Charlebois, R. L., J. D. Hofman, L. C. Schalkwyk, W. L. Lam, and W. F. Doolittle. 1989. Genome mapping in halohacteria. Can. J. Microbiol. 35:21-29.
-
(1989)
Can. J. Microbiol.
, vol.35
, pp. 21-29
-
-
Charlebois, R.L.1
Hofman, J.D.2
Schalkwyk, L.C.3
Lam, W.L.4
Doolittle, W.F.5
-
13
-
-
0023517503
-
Characterization of pHV2 from Halobacterium volcanii and its use in demonstrating transformation of an archaebacterium
-
Charlebois, R. L., W. L. Lam, S. W. Cline, and W. F. Doolittle. 1987. Characterization of pHV2 from Halobacterium volcanii and its use in demonstrating transformation of an archaebacterium. Proc. Natl. Acad. Sci. USA 84:8530-8534.
-
(1987)
Proc. Natl. Acad. Sci. USA
, vol.84
, pp. 8530-8534
-
-
Charlebois, R.L.1
Lam, W.L.2
Cline, S.W.3
Doolittle, W.F.4
-
14
-
-
0026351181
-
Detailed physical map and set of overlapping clones covering the genome of the archaebacterium Haloferax volcanii DS2
-
Charlebois, R. L., L. C. Schalkwyk, J. D. Hofman, and W. F. Doolittle. 1991. Detailed physical map and set of overlapping clones covering the genome of the archaebacterium Haloferax volcanii DS2. J. Mol. Biol. 222:509-524.
-
(1991)
J. Mol. Biol.
, vol.222
, pp. 509-524
-
-
Charlebois, R.L.1
Schalkwyk, L.C.2
Hofman, J.D.3
Doolittle, W.F.4
-
15
-
-
0028999726
-
Biogenesis, structure, and function of the yeast 20S proteasome
-
Chen, P., and M. Hochstrasser. 1995. Biogenesis, structure, and function of the yeast 20S proteasome. EMBO J. 14:2620-2630.
-
(1995)
EMBO J.
, vol.14
, pp. 2620-2630
-
-
Chen, P.1
Hochstrasser, M.2
-
16
-
-
0030595329
-
Autocatalytic subunit processing couples active site formation in the 20S proteasome to completion of assembly
-
Chen, P., and M. Hochstrasser. 1996. Autocatalytic subunit processing couples active site formation in the 20S proteasome to completion of assembly. Cell 86:961-972.
-
(1996)
Cell
, vol.86
, pp. 961-972
-
-
Chen, P.1
Hochstrasser, M.2
-
17
-
-
0024730742
-
Transformation of the archaebacterium Halobacterium volcanii with genomic DNA
-
Cline, S. W., L. C. Schalkwyk, and W. F. Dooliltle. 1989. Transformation of the archaebacterium Halobacterium volcanii with genomic DNA. J. Bacteriol. 171:4987-4991.
-
(1989)
J. Bacteriol.
, vol.171
, pp. 4987-4991
-
-
Cline, S.W.1
Schalkwyk, L.C.2
Dooliltle, W.F.3
-
18
-
-
0026599015
-
Localizing genes on the map of the genome of Haloferax volcanii, one of the Archaea
-
Cohen, A., W. L. Lam, R. L. Charlebois, W. F. Doolittle, and L. C. Schalkwyk. 1992. Localizing genes on the map of the genome of Haloferax volcanii, one of the Archaea. Proc. Natl. Acad. Sci. USA 89:1602-1606.
-
(1992)
Proc. Natl. Acad. Sci. USA
, vol.89
, pp. 1602-1606
-
-
Cohen, A.1
Lam, W.L.2
Charlebois, R.L.3
Doolittle, W.F.4
Schalkwyk, L.C.5
-
19
-
-
0032508046
-
Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence
-
Cole, S. T., R. Brosch, J. Parkhill, T. Garnier, C. Churcher, D. Harris, S. V. Gordon, K. Eiglmeier, S. Gas, C. E. Barry, 3rd, F. Tekaia, K. Badcock, D. Basham, D. Brown, T. Chillingworth, R. Connor, R. Davies, K. Devlin, T. Feltwell, S. Gentles, N. Hamlin, S. Holroyd, T. Hornsby, K. Jagels, and B. G. Barrell. 1998. Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence. Nature 393:537-544.
-
(1998)
Nature
, vol.393
, pp. 537-544
-
-
Cole, S.T.1
Brosch, R.2
Parkhill, J.3
Garnier, T.4
Churcher, C.5
Harris, D.6
Gordon, S.V.7
Eiglmeier, K.8
Gas, S.9
Barry C.E. III10
Tekaia, F.11
Badcock, K.12
Basham, D.13
Brown, D.14
Chillingworth, T.15
Connor, R.16
Davies, R.17
Devlin, K.18
Feltwell, T.19
Gentles, S.20
Hamlin, N.21
Holroyd, S.22
Hornsby, T.23
Jagels, K.24
Barrell, B.G.25
more..
-
20
-
-
0028593502
-
Phylogenic relationships of the amino acid sequences of prosome (proteasome, MCP) subunits
-
Coux, O., H. G. Nothwang, I. S. Pereira, F. R. Targa, F. Bey, and K. Scherrer. 1994. Phylogenic relationships of the amino acid sequences of prosome (proteasome, MCP) subunits. Mol. Gen. Genet. 245:769-780.
-
(1994)
Mol. Gen. Genet.
, vol.245
, pp. 769-780
-
-
Coux, O.1
Nothwang, H.G.2
Pereira, I.S.3
Targa, F.R.4
Bey, F.5
Scherrer, K.6
-
21
-
-
0030016595
-
Structure and functions of the 20S and 26S proteasomes
-
Coux, O., K. Tanaka, and A. L. Goldberg. 1996. Structure and functions of the 20S and 26S proteasomes. Annu. Rev. Biochem. 65:801-847.
-
(1996)
Annu. Rev. Biochem.
, vol.65
, pp. 801-847
-
-
Coux, O.1
Tanaka, K.2
Goldberg, A.L.3
-
22
-
-
0024972956
-
The multicatalytic proteinase (prosome) is ubiquitous from eukaryotes to archaebacteria
-
Dahlmann, B., F. Kopp, L. Kuehn, B. Niedel, G. Pfeifer, R. Hegerl, and W. Baumeister. 1989. The multicatalytic proteinase (prosome) is ubiquitous from eukaryotes to archaebacteria. FEBS Lett. 251:125-131.
-
(1989)
FEBS Lett.
, vol.251
, pp. 125-131
-
-
Dahlmann, B.1
Kopp, F.2
Kuehn, L.3
Niedel, B.4
Pfeifer, G.5
Hegerl, R.6
Baumeister, W.7
-
23
-
-
0026746240
-
Biochemical properties of the proteasome from Thermoplasma acidophilum
-
Dahlmann, B., L. Kuehn, A. Grizwa, P. Zwickl, and W. Baumeister. 1992. Biochemical properties of the proteasome from Thermoplasma acidophilum. Eur. J. Biochem. 208:789-797.
-
(1992)
Eur. J. Biochem.
, vol.208
, pp. 789-797
-
-
Dahlmann, B.1
Kuehn, L.2
Grizwa, A.3
Zwickl, P.4
Baumeister, W.5
-
24
-
-
0029879480
-
Characterization of the distal promoter element of halobacteria in vivo using saturation mutagenesis and selection
-
Danner, S., and J. Soppa. 1996. Characterization of the distal promoter element of halobacteria in vivo using saturation mutagenesis and selection. Mol. Microbiol. 19:1265-1276.
-
(1996)
Mol. Microbiol.
, vol.19
, pp. 1265-1276
-
-
Danner, S.1
Soppa, J.2
-
25
-
-
0028958071
-
Structural features that stabilized halophilic malate dehydrogenase from an archaebacterium
-
Dym, O., M. Mevarech, and J. L. Sussman. 1995. Structural features that stabilized halophilic malate dehydrogenase from an archaebacterium. Science 267:1344-1346.
-
(1995)
Science
, vol.267
, pp. 1344-1346
-
-
Dym, O.1
Mevarech, M.2
Sussman, J.L.3
-
27
-
-
0028933771
-
Life in unusual environments: Progress in understanding the structure and function of enzymes from extreme halophilic bacteria
-
Eisenberg, H. 1995. Life in unusual environments: progress in understanding the structure and function of enzymes from extreme halophilic bacteria. Arch. Biochem. Biophys. 318:1-5.
-
(1995)
Arch. Biochem. Biophys.
, vol.318
, pp. 1-5
-
-
Eisenberg, H.1
-
28
-
-
0032563113
-
Electrostatic contributions to the stability of halophilic proteins
-
Elcock, A. H., and J. A. McCammon. 1998. Electrostatic contributions to the stability of halophilic proteins. J. Mol. Biol. 280:731-748.
-
(1998)
J. Mol. Biol.
, vol.280
, pp. 731-748
-
-
Elcock, A.H.1
McCammon, J.A.2
-
29
-
-
0029033981
-
Inhibition of proteasome activities and subunit-specific amino-terminal threonine modification by lactacystin
-
Fenteany, G., R. F. Standaert, W. S. Lane, S. Choi, E. J. Corey, and S. L. Schreiber. 1995. Inhibition of proteasome activities and subunit-specific amino-terminal threonine modification by lactacystin. Science 268:726-731.
-
(1995)
Science
, vol.268
, pp. 726-731
-
-
Fenteany, G.1
Standaert, R.F.2
Lane, W.S.3
Choi, S.4
Corey, E.J.5
Schreiber, S.L.6
-
30
-
-
0030010138
-
Insights into protein adaptation to a saturated salt environment from the crystal structure of a halophilic 2Fe-2S ferredoxin
-
Frolow, F., M. Harel, J. L. Sussman, M. Mevarech, and M. Shoham. 1996. Insights into protein adaptation to a saturated salt environment from the crystal structure of a halophilic 2Fe-2S ferredoxin. Nat. Struct. Biol. 3:452-458.
-
(1996)
Nat. Struct. Biol.
, vol.3
, pp. 452-458
-
-
Frolow, F.1
Harel, M.2
Sussman, J.L.3
Mevarech, M.4
Shoham, M.5
-
31
-
-
0032498229
-
The human proteasomal subunit HsC8 induces ring formation of other α-type subunits
-
Gerards, W. L., W. W. de Jong, H. Bloemendal, and W. Boelens. 1998. The human proteasomal subunit HsC8 induces ring formation of other α-type subunits. J. Mol. Biol. 275:113-121.
-
(1998)
J. Mol. Biol.
, vol.275
, pp. 113-121
-
-
Gerards, W.L.1
De Jong, W.W.2
Bloemendal, H.3
Boelens, W.4
-
32
-
-
0030950435
-
The human α-type proteasomal subunit HsC8 forms a double ringlike structure, but does not assemble into proteasome-like particles with the β-type subunits HsDelta or HsBPROS26
-
Gerards, W. L., J. Enzlin, M. Haner, I. L. Hendriks, U. Aebi, H. Bloemendal, and W. Boelens. 1997. The human α-type proteasomal subunit HsC8 forms a double ringlike structure, but does not assemble into proteasome-like particles with the β-type subunits HsDelta or HsBPROS26. J. Biol. Chem. 272:10080-10086.
-
(1997)
J. Biol. Chem.
, vol.272
, pp. 10080-10086
-
-
Gerards, W.L.1
Enzlin, J.2
Haner, M.3
Hendriks, I.L.4
Aebi, U.5
Bloemendal, H.6
Boelens, W.7
-
33
-
-
0000875579
-
The effect of salt concentration on the biochemical reactions of some halophilic bacteria
-
Gibbons, N. E. 1957. The effect of salt concentration on the biochemical reactions of some halophilic bacteria. Can. J. Microbiol. 3:249-255.
-
(1957)
Can. J. Microbiol.
, vol.3
, pp. 249-255
-
-
Gibbons, N.E.1
-
34
-
-
0033118230
-
Regulation by proteolysis: Developmental switches
-
Gottesman, S. 1999. Regulation by proteolysis: developmental switches. Curr. Opin. Microbiol. 2:142-147.
-
(1999)
Curr. Opin. Microbiol.
, vol.2
, pp. 142-147
-
-
Gottesman, S.1
-
35
-
-
0015752468
-
Wetting agents for biological electron microscopy. I. General considerations and negative staining
-
Gregory, D. W., and B. J. Pirie. 1973. Wetting agents for biological electron microscopy. I. General considerations and negative staining. J. Microsc. 99:251-255.
-
(1973)
J. Microsc.
, vol.99
, pp. 251-255
-
-
Gregory, D.W.1
Pirie, B.J.2
-
36
-
-
0030897031
-
Structure of 20s proteasome from yeast at 2.4 Å resolution
-
Groll, M., L. Ditzel, J. Löwe, D. Stock, M. Bochtler, H. D. Bartunik, and R. Huber. 1997. Structure of 20S proteasome from yeast at 2.4 Å resolution. Nature 386:463-471.
-
(1997)
Nature
, vol.386
, pp. 463-471
-
-
Groll, M.1
Ditzel, L.2
Löwe, J.3
Stock, D.4
Bochtler, M.5
Bartunik, H.D.6
Huber, R.7
-
37
-
-
0029867623
-
Proteasomes: Destruction as a programme
-
Hilt, W., and D. H. Wolf. 1996. Proteasomes: destruction as a programme. Trends Biochem. Sci. 21:96-102.
-
(1996)
Trends Biochem. Sci.
, vol.21
, pp. 96-102
-
-
Hilt, W.1
Wolf, D.H.2
-
38
-
-
0027968264
-
Improved shuttle vectors for Haloferax volcanii including a dual-resistance plasmid
-
Holmes, M., F. Pfeifer, and M. Dyall-Smith. 1994. Improved shuttle vectors for Haloferax volcanii including a dual-resistance plasmid. Gene 146:117-121.
-
(1994)
Gene
, vol.146
, pp. 117-121
-
-
Holmes, M.1
Pfeifer, F.2
Dyall-Smith, M.3
-
39
-
-
0020808253
-
Purification and properties of serine protease from Halobacterium halobium
-
Izotova, L. S., A. Y. Strongin, L. N. Chekulaeva, V. E. Sterkin, V. I. Ostoslavskaya, L. A. Lyublinskaya, E. A. Timokhina, and V. M. Stepanov. 1983. Purification and properties of serine protease from Halobacterium halobium. J. Bacteriol. 155:826-830.
-
(1983)
J. Bacteriol.
, vol.155
, pp. 826-830
-
-
Izotova, L.S.1
Strongin, A.Y.2
Chekulaeva, L.N.3
Sterkin, V.E.4
Ostoslavskaya, V.I.5
Lyublinskaya, L.A.6
Timokhina, E.A.7
Stepanov, V.M.8
-
40
-
-
0029903278
-
Dihydrolipoamide dehydrogenase from the halophilic archaeon Haloferax volcanii: Homologous overexpression of the cloned gene
-
Jolley, K. A., E. Rapaport, D. W. Hough, M. J. Danson, W. G. Woods, and M. L. Dyall-Smith. 1996. Dihydrolipoamide dehydrogenase from the halophilic archaeon Haloferax volcanii: homologous overexpression of the cloned gene. J. Bacteriol. 178:3044-3048.
-
(1996)
J. Bacteriol.
, vol.178
, pp. 3044-3048
-
-
Jolley, K.A.1
Rapaport, E.2
Hough, D.W.3
Danson, M.J.4
Woods, W.G.5
Dyall-Smith, M.L.6
-
41
-
-
0027446991
-
Characterization of paralogous and orthologous members of the superoxide dismutase gene family from genera of the halophilic archaebacteria
-
Joshi, P., and P. P. Dennis. 1993. Characterization of paralogous and orthologous members of the superoxide dismutase gene family from genera of the halophilic archaebacteria. J. Bacteriol. 175:1561-1571.
-
(1993)
J. Bacteriol.
, vol.175
, pp. 1561-1571
-
-
Joshi, P.1
Dennis, P.P.2
-
42
-
-
0029941607
-
Halolysin R4, a serine proteinase from the halophilic archaeon Haloferax mediterranei: Gene cloning, expression and structural studies
-
Kamekura, M., Y. Seno, and M. Dyall-Smith. 1996. Halolysin R4, a serine proteinase from the halophilic archaeon Haloferax mediterranei: gene cloning, expression and structural studies. Biochim. Biophys. Acta 1294:159-167.
-
(1996)
Biochim. Biophys. Acta
, vol.1294
, pp. 159-167
-
-
Kamekura, M.1
Seno, Y.2
Dyall-Smith, M.3
-
43
-
-
0026541088
-
Molecular cloning and sequencing of the gene for a halophilic alkaline serine protease (halolysin) from an unidentified halophilic archaea strain (172P1) and expression of the gene in Haloferax volcanii
-
Kamekura, M., Y. Seno, M. L. Holmes, and M. L. Dyall-Smith. 1992. Molecular cloning and sequencing of the gene for a halophilic alkaline serine protease (halolysin) from an unidentified halophilic archaea strain (172P1) and expression of the gene in Haloferax volcanii. J. Bacteriol. 174:736-742.
-
(1992)
J. Bacteriol.
, vol.174
, pp. 736-742
-
-
Kamekura, M.1
Seno, Y.2
Holmes, M.L.3
Dyall-Smith, M.L.4
-
44
-
-
0032580064
-
Complete sequence and gene organization of the genome of a hyper-thermophilic archaebacterium, Pyrococcus horikoshii OT3
-
Kawarabayasi, Y., M. Sawada, H. Horikawa, Y. Haidawa, Y. Hino, S. Yamamoto, M. Sekine, S. Baba, H. Kosugi, A. Hosoyama, Y. Nagai, M. Sakai, K. Ogura, R. Otsuka, H. Nakazawa, M. Takamiya, Y. Ohfuku, T. Funahasi, T. Tanaka, Y. Kudoh, J. Yamazaki, N. Kushida, A. Oguchi, K. Aoki, T. Yoshizawa, Y. Nakamura, F. T. Robb, K. Horikoshi, Y. Masuchi, H. Shizuya, and H. Kikuchi. 1998. Complete sequence and gene organization of the genome of a hyper-thermophilic archaebacterium, Pyrococcus horikoshii OT3. DNA Res. 5:147-155.
-
(1998)
DNA Res.
, vol.5
, pp. 147-155
-
-
Kawarabayasi, Y.1
Sawada, M.2
Horikawa, H.3
Haidawa, Y.4
Hino, Y.5
Yamamoto, S.6
Sekine, M.7
Baba, S.8
Kosugi, H.9
Hosoyama, A.10
Nagai, Y.11
Sakai, M.12
Ogura, K.13
Otsuka, R.14
Nakazawa, H.15
Takamiya, M.16
Ohfuku, Y.17
Funahasi, T.18
Tanaka, T.19
Kudoh, Y.20
Yamazaki, J.21
Kushida, N.22
Oguchi, A.23
Aoki, K.24
Yoshizawa, T.25
Nakamura, Y.26
Robb, F.T.27
Horikoshi, K.28
Masuchi, Y.29
Shizuya, H.30
Kikuchi, H.31
more..
-
45
-
-
0017388369
-
Thermophilic character of enzymes from extreme halophilic bacteria
-
Keradjopoulos, D., and A. W. Holldorf. 1977. Thermophilic character of enzymes from extreme halophilic bacteria. FEMS Microbiol. Lett. 1:179.
-
(1977)
FEMS Microbiol. Lett.
, vol.1
, pp. 179
-
-
Keradjopoulos, D.1
Holldorf, A.W.2
-
46
-
-
0031554717
-
Unusual salt and solvent dependence of a protease from an extreme halophile
-
Kim, J., and J. S. Dordick. 1997. Unusual salt and solvent dependence of a protease from an extreme halophile. Biotech. Bioeng. 55:471-479.
-
(1997)
Biotech. Bioeng.
, vol.55
, pp. 471-479
-
-
Kim, J.1
Dordick, J.S.2
-
47
-
-
0031892541
-
Range of sizes of peptide products generated during degradation of different proteins by archaeal proteasomes
-
Kisselev, A. F., T. N. Akopian, and A. L. Goldberg. 1998. Range of sizes of peptide products generated during degradation of different proteins by archaeal proteasomes. J. Biol. Chem. 273:1982-1989.
-
(1998)
J. Biol. Chem.
, vol.273
, pp. 1982-1989
-
-
Kisselev, A.F.1
Akopian, T.N.2
Goldberg, A.L.3
-
48
-
-
0033525086
-
The sizes of peptides generated from protein by mammalian 26 and 20 S proteasomes. Implications for understanding the degradative mechanism and antigen presentation
-
Kisselev, A. F., T. N. Akopian, K. M. Woo, and A. L. Goldberg. 1999. The sizes of peptides generated from protein by mammalian 26 and 20 S proteasomes. Implications for understanding the degradative mechanism and antigen presentation. J. Biol. Chem. 274:3363-3371.
-
(1999)
J. Biol. Chem.
, vol.274
, pp. 3363-3371
-
-
Kisselev, A.F.1
Akopian, T.N.2
Woo, K.M.3
Goldberg, A.L.4
-
49
-
-
0031458333
-
The complete genome sequence of the hyperthermophilic, sulphate-reducing archaeon Archaeoglobus fulgidus
-
Klenk, H. P., R. A. Clayton, J. F. Tomb, O. White, K. E. Nelson, K. A. Ketchum, R. J. Dodson, M. Gwinn, E. K. Hickey, J. D. Peterson, D. L. Richardson, A. R. Kerlavage, D. E. Graham, N. C. Kyrpides, R. D. Fleischmann, J. Quackenbush, N. H. Lee, G. G. Sutton, S. Gill, E. F. Kirkness, B. A. Dougherty, K. McKenney, M. D. Adams, B. Loftus, and J. C. Venter. 1997. The complete genome sequence of the hyperthermophilic, sulphate-reducing archaeon Archaeoglobus fulgidus. Nature 390:364-370.
-
(1997)
Nature
, vol.390
, pp. 364-370
-
-
Klenk, H.P.1
Clayton, R.A.2
Tomb, J.F.3
White, O.4
Nelson, K.E.5
Ketchum, K.A.6
Dodson, R.J.7
Gwinn, M.8
Hickey, E.K.9
Peterson, J.D.10
Richardson, D.L.11
Kerlavage, A.R.12
Graham, D.E.13
Kyrpides, N.C.14
Fleischmann, R.D.15
Quackenbush, J.16
Lee, N.H.17
Sutton, G.G.18
Gill, S.19
Kirkness, E.F.20
Dougherty, B.A.21
McKenney, K.22
Adams, M.D.23
Loftus, B.24
Venter, J.C.25
more..
-
50
-
-
0030877378
-
Inactivation of the 20S proteasome in Mycobacterium smegmatis
-
Knipfer, N., and T. E. Shrader. 1997. Inactivation of the 20S proteasome in Mycobacterium smegmatis. Mol. Microbiol. 25:375-383.
-
(1997)
Mol. Microbiol.
, vol.25
, pp. 375-383
-
-
Knipfer, N.1
Shrader, T.E.2
-
51
-
-
0014949207
-
Cleavage of structural proteins during the assembly of the head of bacteriophage T4
-
Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685.
-
(1970)
Nature
, vol.227
, pp. 680-685
-
-
Laemmli, U.K.1
-
52
-
-
0028987147
-
Genomic stability in the archaeae Haloferax volcanii and Haloferax mediterranei
-
Lopez-Garcia, P., A. St. Jean, R. Amils, and R. L. Charlebois. 1995. Genomic stability in the archaeae Haloferax volcanii and Haloferax mediterranei. J. Bacteriol. 177:1405-1408.
-
(1995)
J. Bacteriol.
, vol.177
, pp. 1405-1408
-
-
Lopez-Garcia, P.1
St Jean, A.2
Amils, R.3
Charlebois, R.L.4
-
53
-
-
0029042511
-
Crystal structure of the 20S proteasome from the archaeon T. acidophilum at 3.4 Å resolution
-
Löwe, J., D. Stock, B. Jap, P. Zwickl, W. Baumeister, and R. Huber. 1995. Crystal structure of the 20S proteasome from the archaeon T. acidophilum at 3.4 Å resolution. Science 268:533-539.
-
(1995)
Science
, vol.268
, pp. 533-539
-
-
Löwe, J.1
Stock, D.2
Jap, B.3
Zwickl, P.4
Baumeister, W.5
Huber, R.6
-
54
-
-
0030867486
-
Self-compartmentalizing proteases
-
Lupas, A., J. M. Flanagan, T. Tamura, and W. Baumeister. 1997. Self-compartmentalizing proteases. Trends. Biochem. Sci. 22:399-404.
-
(1997)
Trends. Biochem. Sci.
, vol.22
, pp. 399-404
-
-
Lupas, A.1
Flanagan, J.M.2
Tamura, T.3
Baumeister, W.4
-
56
-
-
0031918337
-
Biochemical characterization of the 20S proteasome from the methanoarchaeon Methanosarcina thermophila
-
Maupin-Furlow, J. A., H. C. Aldrich, and J. G. Ferry. 1998. Biochemical characterization of the 20S proteasome from the methanoarchaeon Methanosarcina thermophila. J. Bacteriol. 180:1480-1487.
-
(1998)
J. Bacteriol.
, vol.180
, pp. 1480-1487
-
-
Maupin-Furlow, J.A.1
Aldrich, H.C.2
Ferry, J.G.3
-
57
-
-
0028810102
-
A proteasome from the methanogenic archaeon Methanosarcina thermophila
-
Maupin-Furlow, J. A., and J. G. Ferry. 1995. A proteasome from the methanogenic archaeon Methanosarcina thermophila. J. Biol. Chem. 270:28617-28622.
-
(1995)
J. Biol. Chem.
, vol.270
, pp. 28617-28622
-
-
Maupin-Furlow, J.A.1
Ferry, J.G.2
-
58
-
-
34250398425
-
Halobacterium volcanii spec. Nov., a Dead Sea Halobacterium with a moderate salt requirement
-
Mullakhanbhai, M. S., and H. Larsen. 1975. Halobacterium volcanii spec. nov., a Dead Sea Halobacterium with a moderate salt requirement. Arch. Microbiol. 104:207-214.
-
(1975)
Arch. Microbiol.
, vol.104
, pp. 207-214
-
-
Mullakhanbhai, M.S.1
Larsen, H.2
-
59
-
-
0031707009
-
The 20S proteasome of Streptomyces coelicolor
-
Nagy, I., T. Tamura, J. Vanderleyden, W. Baumeister, and R. De Mot. 1998. The 20S proteasome of Streptomyces coelicolor. J. Bacteriol. 180:5448-5453.
-
(1998)
J. Bacteriol.
, vol.180
, pp. 5448-5453
-
-
Nagy, I.1
Tamura, T.2
Vanderleyden, J.3
Baumeister, W.4
De Mot, R.5
-
60
-
-
0029558611
-
Nuclear localization signals of human and Thermoplasma proteasomal a subunits are functional in vitro
-
Nederlof, P. M., H.-R. Wang, and W. Baumeister. 1995. Nuclear localization signals of human and Thermoplasma proteasomal a subunits are functional in vitro. Proc. Natl. Acad. Sci. USA 92:12060-12064.
-
(1995)
Proc. Natl. Acad. Sci. USA
, vol.92
, pp. 12060-12064
-
-
Nederlof, P.M.1
Wang, H.-R.2
Baumeister, W.3
-
61
-
-
0025674423
-
An expression vector for the archaebacterium Haloferax volcanii
-
Nieuwlandt, D. T., and C. J. Daniels. 1990. An expression vector for the archaebacterium Haloferax volcanii. J. Bacteriol. 172:7104-7110.
-
(1990)
J. Bacteriol.
, vol.172
, pp. 7104-7110
-
-
Nieuwlandt, D.T.1
Daniels, C.J.2
-
62
-
-
0014470465
-
Proteolytic enzymes from extremely halophilic bacteria
-
Norberg, P., and B. V. Hofsten. 1969. Proteolytic enzymes from extremely halophilic bacteria. J. Gen. Microbiol. 55:251-256.
-
(1969)
J. Gen. Microbiol.
, vol.55
, pp. 251-256
-
-
Norberg, P.1
Hofsten, B.V.2
-
63
-
-
0027981366
-
A transcriptional reporter for in vivo promoter analysis in the archaeon Haloferax volcanii
-
Palmer, J. R., and C. J. Daniels. 1994. A transcriptional reporter for in vivo promoter analysis in the archaeon Haloferax volcanii. Appl. Environ. Microbiol. 60:3867-3869.
-
(1994)
Appl. Environ. Microbiol.
, vol.60
, pp. 3867-3869
-
-
Palmer, J.R.1
Daniels, C.J.2
-
64
-
-
0028330841
-
Proteasomes: Multisubunit proteinases common to Thermoplasma and eukaryotes
-
Pühler, G., F. Pitzer, P. Zwickl, and W. Baumeister. 1994. Proteasomes: multisubunit proteinases common to Thermoplasma and eukaryotes. Syst. Appl. Microbiol. 16:734-741.
-
(1994)
Syst. Appl. Microbiol.
, vol.16
, pp. 734-741
-
-
Pühler, G.1
Pitzer, F.2
Zwickl, P.3
Baumeister, W.4
-
65
-
-
0032006110
-
Intracellular distribution of proteasomes
-
Rivett, A. J. 1998. Intracellular distribution of proteasomes. Curr. Opin. Immunol. 10:110-114.
-
(1998)
Curr. Opin. Immunol.
, vol.10
, pp. 110-114
-
-
Rivett, A.J.1
-
66
-
-
0028414129
-
Catalytic properties and potential of an extracellular protease from an extreme halophile
-
Ryu, K., and J. S. Dordlck. 1994. Catalytic properties and potential of an extracellular protease from an extreme halophile. Enzyme Microb. Technol. 16:266-275.
-
(1994)
Enzyme Microb. Technol.
, vol.16
, pp. 266-275
-
-
Ryu, K.1
Dordlck, J.S.2
-
68
-
-
0030481129
-
Analysis of mammalian 20S proteasome biogenesis: The maturation of β-subunits is an ordered two-step mechanism involving autocatalysis
-
Schmidtke, G., R. Kraft, S. Kostka, P. Henklein, C. Frommel, J. Löwe, R. Huber, P. M. Kloetzel, and M. Schmidt. 1996. Analysis of mammalian 20S proteasome biogenesis: the maturation of β-subunits is an ordered two-step mechanism involving autocatalysis. EMBO J. 15:6887-6898.
-
(1996)
EMBO J.
, vol.15
, pp. 6887-6898
-
-
Schmidtke, G.1
Kraft, R.2
Kostka, S.3
Henklein, P.4
Frommel, C.5
Löwe, J.6
Huber, R.7
Kloetzel, P.M.8
Schmidt, M.9
-
69
-
-
0025115569
-
Efficient high-performance liquid chromatographic system for the purification of halobacterial serine protease
-
Schmitt, W., U. Rdest, and W. Goebel. 1990. Efficient high-performance liquid chromatographic system for the purification of halobacterial serine protease. J. Chromatogr. 521:211-220.
-
(1990)
J. Chromatogr.
, vol.521
, pp. 211-220
-
-
Schmitt, W.1
Rdest, U.2
Goebel, W.3
-
70
-
-
0029060166
-
Proteasome from Thermoplasma acidophilum: A threonine protease
-
Seemüller, E., A. Lupas, D. Stock, J. Lowe, R. Huber, and W. Baumeister. 1995. Proteasome from Thermoplasma acidophilum: a threonine protease. Science 268:579-582.
-
(1995)
Science
, vol.268
, pp. 579-582
-
-
Seemüller, E.1
Lupas, A.2
Stock, D.3
Lowe, J.4
Huber, R.5
Baumeister, W.6
-
71
-
-
0029789918
-
Autocatalytic processing of the 20S proteasome
-
Seemüller, E., A. Lupas, and W. Baumeister. 1996. Autocatalytic processing of the 20S proteasome. Nature 382:468-470.
-
(1996)
Nature
, vol.382
, pp. 468-470
-
-
Seemüller, E.1
Lupas, A.2
Baumeister, W.3
-
72
-
-
85006102659
-
Cytoplasmic particles and aminoacyl transferase I activity
-
Shelton, E., E. L. Kuff, E. S. Maxwell, and J. T. Harrington. 1970. Cytoplasmic particles and aminoacyl transferase I activity. J. Cell Biol. 45:1-8.
-
(1970)
J. Cell Biol.
, vol.45
, pp. 1-8
-
-
Shelton, E.1
Kuff, E.L.2
Maxwell, E.S.3
Harrington, J.T.4
-
73
-
-
15644383855
-
Complete genome sequence of Methanobacterium thermo-autotrophicum ΔH: Functional analysis and comparative genomics
-
Smith, D. R., L. A. Doucette-Stamm, C. Deloughery, H. Lee, J. Dubois, T. Aldredge, R. Bashirzadeh, D. Blakely, R. Cook, K. Gilbert, D. Harrison, L. Hoang, P. Keagle, W. Lumm, B. Pothier, D. Qiu, R. Spadafora, R. Vicaire, Y. Wang, J. Wierzbowski, R. Gibson, N. Jiwani, A. Caruso, D. Bush, and J. N. Reeve. 1997. Complete genome sequence of Methanobacterium thermo-autotrophicum ΔH: functional analysis and comparative genomics. J. Bacteriol. 179:7135-7155.
-
(1997)
J. Bacteriol.
, vol.179
, pp. 7135-7155
-
-
Smith, D.R.1
Doucette-Stamm, L.A.2
Deloughery, C.3
Lee, H.4
Dubois, J.5
Aldredge, T.6
Bashirzadeh, R.7
Blakely, D.8
Cook, R.9
Gilbert, K.10
Harrison, D.11
Hoang, L.12
Keagle, P.13
Lumm, W.14
Pothier, B.15
Qiu, D.16
Spadafora, R.17
Vicaire, R.18
Wang, Y.19
Wierzbowski, J.20
Gibson, R.21
Jiwani, N.22
Caruso, A.23
Bush, D.24
Reeve, J.N.25
more..
-
74
-
-
0016700864
-
Detection of specific sequences among DNA fragments separated by gel electrophoresis
-
Southern, E. M. 1975. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J. Mol. Biol. 98:503-517.
-
(1975)
J. Mol. Biol.
, vol.98
, pp. 503-517
-
-
Southern, E.M.1
-
75
-
-
0026734617
-
A serine proteinase of an archaebacterium, Halobacterium mediterranei. A homologue of eubacterial subtilisins
-
Stepanov, V. M., G. N. Rudenskaya, L. P. Revina, Y. B. Gryaznova, E. N. Lysogorskaya, I. Y. Filippova, and I. I. Ivanova. 1992. A serine proteinase of an archaebacterium, Halobacterium mediterranei. A homologue of eubacterial subtilisins. Biochem. J. 285:281-286.
-
(1992)
Biochem. J.
, vol.285
, pp. 281-286
-
-
Stepanov, V.M.1
Rudenskaya, G.N.2
Revina, L.P.3
Gryaznova, Y.B.4
Lysogorskaya, E.N.5
Filippova, I.Y.6
Ivanova, I.I.7
-
76
-
-
0029328494
-
The first characterization of a eubacterial proteasome: The 20S complex of Rhodococcus
-
Tamura, T., I. Nagy, A. Lupas, F. Lottspelch, Z. Cejka, G. Schoofs, K. Tanaka, R. DeMot, and W. Baumeister. 1995. The first characterization of a eubacterial proteasome: the 20S complex of Rhodococcus. Curr. Biol. 5:766-774.
-
(1995)
Curr. Biol.
, vol.5
, pp. 766-774
-
-
Tamura, T.1
Nagy, I.2
Lupas, A.3
Lottspelch, F.4
Cejka, Z.5
Schoofs, G.6
Tanaka, K.7
DeMot, R.8
Baumeister, W.9
-
77
-
-
0031819536
-
The MHC class I ligand-generating system: Roles of immunoproteasomes and the interferon-gamma-inducible proteasome activator PA28
-
Tanaka, K., and M. Kasahara. 1998. The MHC class I ligand-generating system: roles of immunoproteasomes and the interferon-gamma-inducible proteasome activator PA28. Immunol. Rev. 163:161-176.
-
(1998)
Immunol. Rev.
, vol.163
, pp. 161-176
-
-
Tanaka, K.1
Kasahara, M.2
-
78
-
-
0027968068
-
CLUSTAL W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice
-
Thompson, J. D., D. G. Higgins, and T. J. Gibson. 1994. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 22:4673-4680.
-
(1994)
Nucleic Acids Res.
, vol.22
, pp. 4673-4680
-
-
Thompson, J.D.1
Higgins, D.G.2
Gibson, T.J.3
-
79
-
-
0028365133
-
Processive degradation of proteins by the ATP-dependent CIp protease from Escherichia coli: Requirement for the multiple array of active sites in CIpB but not ATP hydrolysis
-
Thompson, M. W., S. K. Singh, and M. R. Maurizi. 1994. Processive degradation of proteins by the ATP-dependent CIp protease from Escherichia coli: requirement for the multiple array of active sites in CIpB but not ATP hydrolysis. J. Biol. Chem. 269:18209-18215.
-
(1994)
J. Biol. Chem.
, vol.269
, pp. 18209-18215
-
-
Thompson, M.W.1
Singh, S.K.2
Maurizi, M.R.3
-
80
-
-
0015522277
-
Fluorescamine: A reagent for assay of amino acids, peptides, proteins, and primary amines in the picomole range
-
Udenfriend, S., S. Stein, P. Bohlen, W. Dairman, W. Leimgruber, and M. Weigele. 1972. Fluorescamine: a reagent for assay of amino acids, peptides, proteins, and primary amines in the picomole range. Science 178:871-872.
-
(1972)
Science
, vol.178
, pp. 871-872
-
-
Udenfriend, S.1
Stein, S.2
Bohlen, P.3
Dairman, W.4
Leimgruber, W.5
Weigele, M.6
-
81
-
-
0026587208
-
Codon usage tabulated from the GenBank genetic sequence data
-
Wada, K., Y. Wada, F. Ishibashi, T. Gojobori, and T. Ikelmura. 1992. Codon usage tabulated from the GenBank genetic sequence data. Nucleic Acids Res. 20:2111-2118.
-
(1992)
Nucleic Acids Res.
, vol.20
, pp. 2111-2118
-
-
Wada, K.1
Wada, Y.2
Ishibashi, F.3
Gojobori, T.4
Ikelmura, T.5
-
82
-
-
0030691115
-
The structure of ClpP at 2.3 Å resolution suggests a model for ATP-dependent proteolysis
-
Wang, J., J. A. Hartling, and J. M. Flanagan. 1997. The structure of ClpP at 2.3 Å resolution suggests a model for ATP-dependent proteolysis. Cell 91: 447-456.
-
(1997)
Cell
, vol.91
, pp. 447-456
-
-
Wang, J.1
Hartling, J.A.2
Flanagan, J.M.3
-
83
-
-
0028985861
-
Conformational constraints in protein degradation by the 20S proteasome
-
Wenzel, T., and W. Baumeister. 1995. Conformational constraints in protein degradation by the 20S proteasome. Nat. Struct. Biol. 2:199-204.
-
(1995)
Nat. Struct. Biol.
, vol.2
, pp. 199-204
-
-
Wenzel, T.1
Baumeister, W.2
-
84
-
-
0039660001
-
Dissecting the assembly pathway of the 20S proteasome
-
Zühl, F., E. Seemüller, R. Golbik, and W. Baumeister. 1997. Dissecting the assembly pathway of the 20S proteasome. FEBS Lett. 418:189-194.
-
(1997)
FEBS Lett.
, vol.418
, pp. 189-194
-
-
Zühl, F.1
Seemüller, E.2
Golbik, R.3
Baumeister, W.4
-
85
-
-
0039660006
-
Subunit topology of the Rhodococcus proteasome
-
Zühl, F., T. Tamura, I. Dolenc, Z. Cejka, I. Nagy, R. DeMot, W. Baumeister, and R. De Mot. 1997. Subunit topology of the Rhodococcus proteasome. FEBS Lett. 400:83-90.
-
(1997)
FEBS Lett.
, vol.400
, pp. 83-90
-
-
Zühl, F.1
Tamura, T.2
Dolenc, I.3
Cejka, Z.4
Nagy, I.5
DeMot, R.6
Baumeister, W.7
De Mot, R.8
-
86
-
-
0026600718
-
Primary structure of the Thermoplasma proteasome and its implications for the structure, function, and evolution of the multicatalytic proteinase
-
Zwickl, P., A. Grizwa, G. Puhler, B. Dahlmann, F. Lottspeich, and W. Baumeister. 1992. Primary structure of the Thermoplasma proteasome and its implications for the structure, function, and evolution of the multicatalytic proteinase. Biochemistry 31:964-972.
-
(1992)
Biochemistry
, vol.31
, pp. 964-972
-
-
Zwickl, P.1
Grizwa, A.2
Puhler, G.3
Dahlmann, B.4
Lottspeich, F.5
Baumeister, W.6
-
88
-
-
0025957995
-
Cloning and sequencing of the gene encoding the large (α-) subunit of the proteasome from Thermoplasma acidophilum
-
Zwickl, P., F. Lottspeich, B. Dahlmann, and W. Baumeister. 1991. Cloning and sequencing of the gene encoding the large (α-) subunit of the proteasome from Thermoplasma acidophilum. FEBS Lett. 278:217-221.
-
(1991)
FEBS Lett.
, vol.278
, pp. 217-221
-
-
Zwickl, P.1
Lottspeich, F.2
Dahlmann, B.3
Baumeister, W.4
|