Signal-dependent phosphorylation of the membrane-bound NarX two- component sensor-transmitter protein of Escherichia coli: Nitrate elicits a superior anion ligand response compared to nitrite

Journal of Bacteriology

Volumn 181, Issue 17, 1999, Pages 5309-5316

  Lee, Angela I ^a   Delgado, Asunción ^a   Gunsalus, Robert P ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANION; BACTERIAL PROTEIN; NITRITE; PROTEIN NARX; UNCLASSIFIED DRUG;

ANAEROBIC METABOLISM; ARTICLE; AUTOPHOSPHORYLATION; BACTERIAL MEMBRANE; BIOSENSOR; CONCENTRATION RESPONSE; ENZYME ACTIVITY; ESCHERICHIA COLI; FERMENTATION; LIGAND BINDING; NONHUMAN; PRIORITY JOURNAL; PROTEIN PHOSPHORYLATION; SIGNAL TRANSDUCTION; TIME;

ESCHERICHIA COLI;

EID: 0032835113     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.181.17.5309-5316.1999     Document Type: Article

References (24)

1. Bock, A., and G. Sawers. 1996. Fermentation, p. 262-282. In F. C. Neidhardt, R. Curtiss III, J. L. Ingraham, E. C. C. Lin, K. B. Low, B. Magasanik, W. S. Riley, M. Schaechter, and H. E. Umbarger (ed.), Escherichia coli and Salmonella: cellular and molecular biology, vol. 1. American Society for Microbiology, Washington, D.C.
2. Cavicchioli, R., R. C. Chiang, L. V. Kalman, and R. P. Gunsalus. 1996. Role of the periplasmic domain of the Escherichia coli NarX sensor-transmitter protein in nitrate-dependent signal transduction and gene regulation. Mol. Microbiol. 21:901-911.
3. Cavicchioli, R., I. Schroder, M. Constanti, and R. P. Gunsalus. 1995. The NarX and NarQ sensor-transmitter proteins of Escherichia coli each require two conserved histidines for nitrate-dependent signal transduction to NarL. J. Bacteriol. 177:2416-2424.
4. Chiang, R. C., R. Cavicchioli, and R. P. Gunsalus. 1992. Identification and characterization of narQ, a second nitrate sensor for nitrate-dependent gene regulation in Escherichia coli. Mol. Microbiol. 6:1913-1923.
5. Chiang, R. C., R. Cavicchioli, and R. P. Gunsalus. 1997. 'Locked-on' and 'locked-off' signal transduction mutations in the periplasmic domain of the Escherichia coli NarQ and NarX sensors affect nitrate-and nitrite-dependent regulation by NarL and NarP. Mol. Microbiol. 24:1049-1060.
6. Darwin, A. J., and V. Stewart. 1996. The Nar modulon systems: nitrate and nitrite regulation of anaerobic gene expression, p. 333-359. In E. C. C. Lin and A. S. Lynch (ed.), Regulation of gene expression in E. coli. R. G. Landes Company, Austin, Tex.
7. Gennis, R., and V. Stewart. 1996. Respiration, p. 217-261. In F. C. Neidhardt, R. Curtiss III, J. L. Ingraham, E. C. C. Lin, K. B. Low, B. Magasanik, W. S. Riley, M. Schaechter, and M. E. Umbarger (ed.), Escherichia coli and Salmonella: cellular and molecular biology, vol. 1. American Society for Microbiology, Washington, D.C.
8. Gunsalus, R. P. 1992. Control of electron flow in Escherichia coli: coordinated transcription of respiratory pathway genes. J. Bacteriol. 174:7069-7074.
9. Habeeb, A. F. S. A. 1972. Reaction of protein sulfhydryl groups with Ellman's reagent. Methods Enzymol. 25:457-464.
10. 1 nitrite reductase) in Pseudomonas stutzeri during the transition from aerobic respiration to denitrification: evidence for a denitrification-specific nitrate-and nitrite-responsive regulatory system. J. Bacteriol. 181:161-166.
11. Hess, J. F., R. B. Bourret, and M. I. Simon. 1991. Phosphorylation assays for proteins of the two-component regulatory system controlling chemotaxis in Escherichia coli. Methods Enzymol. 200:188-204.
12. Kalman, L. V., and R. P. Gunsalus. 1990. Nitrate-and molybdenum-independent signal transduction mutations in narX that alter regulation of anaerobic respiratory genes in Escherichia coli. J. Bacteriol. 172:7049-7056.
13. Miller, J. H. 1972. Experiments in molecular genetics. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
14. Olami, Y., A. Rimon, Y. Gerchman, A. Rothman, and E. Padan. 1997. Histidine 225, a residue of the NhaA-Na+/H+ antiporter of Escherichia coli, is exposed and faces the cell exterior. J. Biol. Chem. 272:1761-1768.
15. Parkinson, J. S., and E. C. Kofoid. 1992. Communication modules in bacterial signaling proteins. Annu. Rev. Genet. 26:71-112.
16. Riordan, J. F., and B. L. Vallee. 1972. Reactions with N-ethylmaleimide and p-mercuribenzoate. Methods Enzymol. 25:449-455.
17. Sanger, F., S. Nicklen, and A. R. Coulson. 1977. DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74:5463-5467.
18. Schroeder, I., S. Darie, and R. P. Gunsalus. 1993. Activation of the Escherichia coli nitrate reductase (narGHJI) operon by NarL and Fnr requires integration host factor. J. Biol. Chem. 268:771-774.
19. Schroeder, I., C. D. Wolin, R. Cavicchioli, and R. P. Gunsalus. 1994. Phosphorylation and dephosphorylation of the NarQ, NarX, and NarL proteins of the nitrate-dependent two-component regulatory system of Escherichia coli. J. Bacteriol. 176:4985-4992.
20. Stewart, V. 1993. Nitrate regulation of anaerobic respiratory gene expression in Escherichia coli. Mol. Microbiol. 9:425-434.
21. Towbin, H., T. Staehelin, and J. Gordon. 1979. Electrophoretic transfer of proteins from polyacrylamide genes to nitrocellulose sheets: procedure and some applications. Proc. Natl. Acad. Sci. USA 76:4350-4354.
22. Walker, M. S., and J. A. DeMoss. 1993. Phosphorylation and dephosphorylation catalyzed in vitro by purified components of the nitrate sensing system, NarX and NarL. J. Biol. Chem. 268:8391-8393.
23. Wang, H., C.-P. Tseng, and R. P. Gunsalus. 1999. The napF and narG nitrate reductase operons in Escherichia coli are differentially expressed in response to submicromolar concentrations of nitrate but not nitrite. J. Bacteriol. 181:5303-5308.
24. Williams, S. B., and V. Stewart. 1997. Discrimination between structurally related ligands nitrate and nitrite controls autokinase activity of the NarX transmembrane signal transducer of Escherichia coli K-12. Mol. Microbiol. 26:911-925.