Spectrophotometric measurement of hydroperoxides at increased sensitivity by oxidation of Fe2+ in the presence of xylenol orange

Free Radical Research

Volumn 31, Issue 3, 1999, Pages 237-244

  Deiana, Luca ^a   Carru, Ciriaco ^a   Pes, Gianni ^a   Tadolini, Bruna ^a,b  

^a UNIVERSITY OF SASSARI   (Italy)

^b Sezione di Microbiologia clinica e sperimentale   (Italy)

Author keywords

Hydrogen peroxide; LDL; Lipid hydroperoxide; Spectrophotometric method; Xylenol orange

Indexed keywords

FERROUS ION; HYDROPEROXIDE; LIPOSOME; LOW DENSITY LIPOPROTEIN; PEROXIDE; PHOSPHATIDYLCHOLINE; SUCROSE; UNCLASSIFIED DRUG; XYLENE; XYLENOL ORANGE;

ARTICLE; CHEMICAL REACTION; LIPID PEROXIDATION; MATHEMATICS; MEASUREMENT; OXIDATION; PH; SPECTROPHOTOMETRY;

1,2-DIPALMITOYLPHOSPHATIDYLCHOLINE; BENZENE DERIVATIVES; FLUORESCENT DYES; FREE RADICAL SCAVENGERS; HUMANS; HYDROGEN PEROXIDE; IRON; LIPID PEROXIDES; LIPOPROTEINS, LDL; LIPOSOMES; OXIDATION-REDUCTION; SENSITIVITY AND SPECIFICITY; SPECTROPHOTOMETRY; TERT-BUTYLHYDROPEROXIDE; XYLENES;

EID: 0032832261     PISSN: 10715762     EISSN: None     Source Type: Journal    
DOI: 10.1080/10715769900300801     Document Type: Article

References (24)

1. B. Halliwell and J.M.C. Gutteridge (1989) Free radicals, aging, and disease. In Free Radicals in Biology and Medicine, 2nd edn., Oxford University Press (Clarendon), London/ New York, pp. 416-494.
2. B. Tadolini and G. Hakim (1996) The mechanism of iron (III) stimulation of lipid peroxidation. Free Radical Research 25, 221-227.
3. B. Tadolini, L. Cabrini, C. Menna, G.G. Pinna and G. Hakim (1997) Iron (III) stimulation of lipid hydroperoxide-dependent lipid peroxidation. Free Radical Research 27, 563-576.
4. B. Frei and J.M. Gaziano (1993) Content of antioxidants, preformed lipid hydroperoxides, and cholesterol as predictors of the susceptibility of human LDL to metal ion-dependent and -independent oxidation. Journal of Lipid Research 34, 2135-2145.
5. V.J. O'Leary, V.M. Darley-Usmar, L.J. Russell and D. Stone (1992) Pro-oxidant effects of lipoxygenase-derived peroxides on the copper-initiated oxidation of low-density lipoprotein. Biochemical Journal 282, 631-634.
6. 2+ catalyzed oxidation. Biochemical Journal 314, 577-585.
7. M. Hicks and J.M. Gebicki (1979) A spectrophotometric method for the determination of lipid hydroperoxides. Analytical Biochemistry 99, 249-253.
8. H. Ohkawa, N. Ohishi and K.J. Yagi (1978) Reaction of linoleic acid hydroperoxide with thiobarbituric acid. Journal of Lipid Research 19, 1053-1057.
9. J.A. Beuge and S.D. Aust (1978) Microsomal lipid peroxidation. Methods in Enzymology 52, 302-310.
10. Z.Y. Jiang, A.C.S. Woollard and S.P. Wolff (1990) Hydrogen peroxide production during experimental protein glycation. FEBS Letters 268, 69-71.
11. 2+ in the presence of xylenol orange. Comparison with the TBA assay and an iodometric method. Lipids 26, 853-856.
12. Z.Y. Jiang, J.V. Hunt and S.P. Wolff (1992) Ferrous ion oxidation in the presence of xylenol orange for detection of lipid hydroperoxide in low density lipoprotein. Analytical Biochemistry 202, 384-389.
13. J.V. Hunt, Z.Y. Jiang and S.P. Wolff (1992) Formation of hydrogen peroxide by lens proteins: protein-derived hydrogen peroxide as a potential mechanism of oxidative insult to the lens. Free Radical Biology and Medicine 13, 319-323.
14. S.P. Wolff (1994) Ferrous ion oxidation in presence of ferric ion indicator xylenol orange for measurement of hydroperoxides. Methods in Enzymology 233, 182-189.
15. 2 in irradiated solutions. Microchemical Journal 18, 363-374.
16. L. Cavallini, M. Valenti and A. Bindoli (1983) NADH and NADPH inhibit lipid peroxidation promoted by hydroperoxides in rat liver microsomes. Biochimica and Biophysica Acta 752, 339-345.
17. B. Tadolini, D. Fiorentini, L. Landi and L. Cabrini (1989) Lipid peroxidation. Definition of experimental conditions for selective study of the propagation and termination phases. Free Radical Research Communications 5, 245-252.
18. R.A. Klein (1970) The detection of oxidation in liposome preparations. Biochimica and Biophysica Acta 210, 468-489.
19. M.J. Hope, M.B. Bally, G. Webb and P.R. Cullis (1985) Production of large unilamellar vesicles by a rapid extrusion procedure. Characterization of size distribution, trapped volume and ability to mantain a membrane potential. Biochimica and Biophysica Acta 812, 55-65.
20. G.V. Marinetti (1962) Chromatographic separation, identification and analysis of phosphatides. Journal of Lipid Research 3, 1-20.
21. J. Himber, E. Buhler, D. Moll and U.K. Moser (1995) Low density lipoprotein for oxidation and metabolic studies. Isolation from small volumes of plasma using a Tabletop centrifuge. Journal of Vitamin and Nutrition Research 65, 137-142.
22. E.G. Bligh and W.J. Dyer (1959) A rapid method of total lipid extraction and purification. Canadian Journal of Biochemistry and Biophysiology 37, 911-917.
23. J. Nourooz-Zadeh, J. Tajaddini-Sarmani, K.L.E. Ling and S.P. Wolff (1996) Low-density lipoprotein is the major carrier of lipid hydroperoxides in plasma. Biochemical Journal 313, 781-786.
24. A. Zamburlini, M. Maiorino, P. Barbera, A.M. Pastorino, A. Roveri, L. Cominacini and F. Ursini (1995) Measurement of lipid hydroperoxides in plasma lipoproteins by a new highly-sensitive 'single photon counting' luminometer. Biochimica and Biophysica Acta 1256, 233-240.