A simple procedure for isolation of Bufo arenarum C3 complement fraction and preparation of antiserum

Comparative Biochemistry and Physiology - C Pharmacology Toxicology and Endocrinology

Volumn 124, Issue 1, 1999, Pages 1-5

  Llanos, Ricardo J ^a   Miceli, Dora C ^a   Valz Gianinet, Jorge N ^a   Whitacre, Cecilia M ^b  

^a UNIVERSIDAD NACIONAL DE TUCUMÁN   (Argentina)

^b CASE WESTERN RESERVE UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Amphibian; Antiserum; Bufo arenarum; C3; Zymosan

Indexed keywords

ANTISERUM; COMPLEMENT COMPONENT C3; ZYMOSAN;

ANIMAL EXPERIMENT; ANIMAL TISSUE; ANTIBODY SPECIFICITY; ARTICLE; CHROMATOGRAPHY; IMMUNOBLOTTING; IMMUNOCHEMISTRY; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN ISOLATION; PROTEIN PROTEIN INTERACTION; PROTEIN PURIFICATION; RABBIT; TECHNIQUE; TOAD;

ANIMALS; ANTIBODY SPECIFICITY; BUFONIDAE; CHEMICAL FRACTIONATION; COMPLEMENT C3; COMPLEMENT C3B; CROSS REACTIONS; HUMANS; IMMUNE SERA; IMMUNOBLOTTING; IMMUNODOMINANT EPITOPES; RABBITS; ZYMOSAN;

EID: 0032832177     PISSN: 07428413     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0742-8413(99)00037-7     Document Type: Article

References (30)

1. 3 from different species. Dev Comp Immunol. 16:1992;63-76.
2. Daha M.R., Stuffer-Heiman M., Kijlstra A., Van-Es L.A. Isolation and characterization of the third component of rat complement. Immunology. 36:1979;63-70.
3. Davis A., Harrison R. Structural characterization of factor I mediated cleavage of the third component of complement. Biochemistry. 21:1982;5745-5749.
4. Ey P.L., Prowse S.J., Jenkin C.R. Isolation of pure IgG2a and IgG2b immunoglobulin from mouse serum using protein A-Sepharose. Immunochemistry. 15:1978;429-436.
5. Fujii T., Sekizawa A., Katagiri C. Characterization of the fourth component of complement in the serum of the clawed frog Xenopus laevis. Immunology. 56:1985;743-750.
6. Fujii T., Nakamura T., Sekizawa A., Tomonaga S. Isolation and characterization of a protein from hagfish serum that is homologous to the third component of the mammalian complement system. J Immunol. 148:1992;117-120.
7. 3 of Xenopus and mammals. Proc Natl Acad Sci USA. 86:1989;1323-1327.
8. Gupta A.K., Rao K.M. A simple method of raising antiserum to the third fraction of mouse serum complement. J Immunol Methods. 35:1980;173-175.
9. Gyongyossy M.I.C., Assimeh S.N. Isolation of the third component of mouse complement. J Immunol. 119:1977;1032-1037.
10. Harrison R., Lachmann P.J. The physiological breakdown of the third component of human complement. Mol Immunol. 17:1980;9-20.
11. Kunnath-Muglia L.M., Chang G.H., Sim R.B., Day A.J., Ezekowitz R.A.B. Characterization of Xenopus laevis complement factor I structure- conservation of modular structure except for an unusual insert not present in human factor I. Mol Immunol. 30:1993;1249-1256.
12. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 27:1970;680-685.
13. 3, the third component of complement. Immunol Today. 9:1988;387-392.
14. 3-ligand interactions. Ann New York Acad Sci. 712:1994;354-357.
15. 3 transcript. Eur J Immunol. 25:1995;572-578.
16. 3b. J Immunol. 122:1979;759-765.
17. Llanos R.J., Whitacre C.M., Medina M., Fernandez S.N., Giunta S., Miceli D.C. Involvement of blood serum in amphibian fertilization. Biocell. 22:1998;67-72.
18. Malaviya A.N., Batra B., Vaishnava S. A simple method for producing monospecific antiserum to beta 1-C fraction (third component of complement) of human serum. Indian J Med Res. 60:1972;416-418.
19. Mavroidis M., Sunyer J.O., Lambris J.D. Isolation, primary structure and evolution of the third component of chicken complement and evidence for a new member of the alpha2-macroglobulin family. J Immunol. 154:1995;2164-2174.
20. Nilsson U.R., Muller-Eberhard H.J. Isolation of β1F-globulin from human serum and its characterization as the fifth component of complement. J Exp Med. 122:1965;277-298.
21. Nonaka M., Fujii T., Kaidoh T., Natsuume-Sakai S., Nohaka M., Yamaguchi N. et al. Purification of a lamprey complement protein homologous to the third component of the mammalian complement system. J Immunol. 133:1984;3242-3249.
22. 3. Immunology. 33:1977;491-499.
23. 3: isolation and characterization of reactions in vitro and during in vivo antigen-antibody interaction. Immunology. 17:1969;695-707.
24. 3 with purified factor I or serum. I. Requirements for factor H versus CR1 cofactor activity. J Immunol. 129:1982;2051-2060.
25. Sekizawa A., Fujii T., Katagiri C. Isolation and characterization of the third component of complement in the serum of the clawed frog, Xenopus laevis. J Immunol. 133:1984;1436-1443.
26. 3). J Immunol. 133:1984;1431-1435.
27. Shin H.S., Mayer M.M. The third component of the guinea pig complement system. I. Purification and characterization. Biochemistry. 7:1968;2991-2996.
28. 3 in the diploid fish Sparus aurata. J Immunol. 158:1997;2813-2821.
29. Whitacre C.M., Perdigon G., Fernandez S.N., Miceli D.C. Role of oviductal pars recta extract on interspecific fertilization between anurans. Dev Growth Differ. 38:1996;203-207.
30. Williams C.A., Chase M.W. Williams C.A., Chase M.W. Methods in immunology and immunochemistry. 4:1977;272 Academic Press, New York.