Molecular design, characterization and pharmacological activity of conjugates of protein proteinase inhibitors

S.T.P. Pharma Sciences

Volumn 9, Issue 1, 1999, Pages 67-82

  Larionova, N I ^a   Moroz, N A ^a   Tyurina, O P ^a  

^a MOSCOW STATE UNIVERSITY   (Russian Federation)

Author keywords

Aprotinin; Birksoybean inhibitor; Bowman; Carrier mediated transport; Conjugate; Peptide delivery; Proteinase inhibitor; Receptor mediated transport

Indexed keywords

DRUG CARRIER; PEPTIDE DERIVATIVE; PROTEIN DERIVATIVE; PROTEINASE INHIBITOR; SERINE PROTEINASE;

CONJUGATE; DRUG ACTIVITY; DRUG BIOAVAILABILITY; DRUG CONJUGATION; DRUG DELIVERY SYSTEM; DRUG DESIGN; DRUG DISTRIBUTION; DRUG SYNTHESIS; DRUG UPTAKE; ENZYME ACTIVATION; REVIEW;

EID: 0032831721     PISSN: 11571489     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Review

References (94)

1. HORL H. and HEIDLAND A. (Eds.) - Proteases: Potential Role in Health and Disease, Advances in Experimental Medicine and Biology, vol. 167. - Plenum Press, New York and London, 1982.
2. SCHNEBLI H.P. and BRAUN N.J. - Protease inhibitors as drugs - In: Proteinase Inhibitors, A.J. Barrett and G. Salvesen Eds., Elsevier Science, Amsterdam, 1986, pp. 613-627.
3. FRITZ H. and WUNDERER G. - Biochemistry and applications of aprotinin, the kallikrein inhibitor from bovine organs. -Arzneim. -Forsch., 33, 479-494, 1983.
4. VERSTRAETE M. - Clinical application of inhibitors of fibrinolysis. - Drugs, 29, 236-261, 1985.
5. LIU J.N., SUN Z., JIANG Y., TANG W. and GUREWICH V. -Inhibition of plasminogen activation by Kunitz-type inhibitors. -Fibrinolysis, 10, suppl. 3, 37, 1996.
6. KOZA M., WALENGA J.M., ROSELLI E., FAREED J. and PIFARRE R. - Aprotinin modulation of tissue factor mediated platelet activation in normal and aspirinized individuals. -Fibrinolysis, 10, suppl. 3, 40, 1996.
7. PINTINGNY D. and DACHARY-PRIGENT J. - Aprotinin can inhibit proteolytic activity of thrombin. A fluorescence and an enzymatic study. - Eur J. Biochem., 207, 89-95, 1992.
8. TRAPNELL J.E., RIGBY C.C., TALBOT C.H. and DUNCAN E.H.L. - A controlled trial of Trasylol in the treatment of acute pancreatitis. - Brit. J. Surg., 61, 177-182, 1974.
9. LARVIN M., MAYER A.D., McMAHON M.J., MULLER-ESTERL W. and FRITZ H. - Intraperitoneal aprotinin therapy for acute pancreatitis. - Biol. Chem. Hoppe-Seyler, 369, suppl., 149-152, 1988.
10. SCHUMANN F. - Aprotinin in the control of medical and surgical bleeding. - Satellite Symposium of the XIII International Congress on Fibrinolysis and Thrombolysis. Aprotinin and Lysine Analogues in the Control of Medical and Surgical Bleeding, Barcelona, 24 June 1996.
11. ROBERT S., WAGNER B.K., BOULANGER M. and RICHER M. - Aprotinin. - Ann. Pharmacother., 30, 372-380, 1996.
12. DAVIS R. and WHITTINGTON R. - Aprotinin. A review of its pharmacology and therapeutic efficacy in reducing blood loss associated with cardiac surgery. - Drugs, 49, 954-983, 1995.
13. LEVY J.H., PIFARRE R., SCHAFF H.V., HORROW J.C., ALBUS R., SPIESS B., ROSENGART T.K., MURRAY J., CLARK R.E., SMITH P., NADEL A., BONNEY S.L. and KLEINFIELD R. - A multicenter double-blind, placebo-controlled trial of aprotinin for reducing blood loss and the requirement for donor blood transfusion in patients undergoing repeat coronary artery bypass grafting. - Circulation, 92, 2236-2244, 1995.
14. ROYSTON D. - Preventing the inflammatory response to open-heart surgery: the role of aprotinin and other protease inhibitors. -Int. J. Cardiology, 53, 11-37, 1996.
15. SOEPARWATA R., HARTMAN A.R., FRERICHMANN U., STEFANO G.B., SCHELD H.H. and BILFINGER T.V. - Aprotinin diminishes inflammatory processes. - Int. J. Cardiology, 26, 1 53, 55-63, 1996.
16. BOROKHOV A.I., RAEVSKAYA T.N. and KUPAVIKH O.S. -Role of aprotinin in prophylaxis of miocardium rupture in patients with acute transmural miocardial infarction. - In: Myocardial Ischaemic Disease, A.I. Borokhov Ed., Smolensk, 1993, pp. 49-52.
17. BIRK Y. - The Bowman-Birk inhibitor. Trypsin and chymotrypsin inhibitor from soybeans. - Int. J. Peptide Protein Res., 25, 113-131, 1985.
18. LARIONOVA N.I. and GLADYSHEVA I.P. - Bowman-Birk family of proteinase inhibitors. - Novosti Nauki i Tekhniki, ser. Biotekhnologiya 8, 20-80, 1990 (Russian).
19. LASKOWSKI M., Jr. and SEALOCK R.W. - Protein proteinase inhibitors, molecular aspects. - In: The Enzymes, P.D. Boyer Ed., 3rd ed., Academic Press, New York, 1971, pp. 375-473.
20. EKLUND K.K. and STEVENS R.L. - Mast cell proteases. - In: Innovations on Proteases and Their Inhibitors, F.X. Aviles Ed., Walterde Gruyter, Berlin, 1993, pp.241-257
21. WARE J.H., WAN X.S., RUBIN H., SCHECHTER N.M. and KENNEDY A.R. - Soybean Bowman-Birk protease inhibitor is a highly effective inhibitor of human mast cell chymase. - Arch. Biochem. Biophys., 344, 133-138, 1997.
22. KATUNUMA N. and KIDO H. - Biological functions of serine proteases in mast cells in allergic inflammation. - J. Cel. Biochem., 38, 291-301, 1988
23. LARIONOVA N.I., GLADYSHEVA I.P., TIKHONOVA T.V. and KAZANSKAYA N.F. - Inhibition of cathepsin G and human granulocyte elastase by multiple forms of a soybean inhibitor of the Bowman-Birk type. - Biochemistry (Moscow), 58, 1046-1052, 1993.
24. GLADYSHEVA I.P., LARIONOVA N.I., GLADYSHEV D.P., TIKHONOVA T.V. and KAZANSKAYA N.F. - A classical Bowman-Birk soybean inhibitor acts as an effective inhibitor of alpha-chymotrypsin and human granulocyte cathepsin G. -Biochemistry (Moscow), 59, 371-374, 1994.
25. LARIONOVA N.I., GLADYSHEVA I.P. and GLADYSHEV D.P. -Human leukocyte elastase inhibition by Bowman-Birk soybean inhibitor. Discrimination of the inhibition mechanism. - FEBS Lett., 404, 245-248, 1997.
26. BIETH J.G. - Elastase: catalytic and biological properties. - In: Regulation of Matrix Accumulation, R.P. Mecham Ed., Academic Press, New York, 1986, pp. 217-320.
27. TIKHONOVA T.V., LARIONOVA N.I., GLADYSHEVA I.P. and KAZANSKAYA N.F. - Bowman-Birk soybean inhibitor as an affinity ligand for isolation of leukocyte elastase: Inhibition of leukocyte elastase-mediated elastin hydrolysis. - Biochemistry (Moscow), 58, 1227-1233, 1993.
28. TIKHONOVA T.V., GLADYSHEVA I.P., KAZANSKAYA N.F. and LARIONOVA N.I. - Hydrolysis of elastin by human leukocyte elastase and cathepsin G: Inhibition by Bowman-Birk-type soybean inhibitor. - Biochemistry (Moscow), 59, 1295-1299, 1994.
29. TIKHONOVA T.V., GLADYSHEVA I.P. and LARIONOVA N.I. -Retardation by the soybean Bowman-Birk Inhibitor of elastin hydrolysis catalyzed by leukocyte proteinases. - FEBS Lett., 362, 225-228, 1995.
30. LARIONOVA N.I., BALABUSHEVITCH N.G. and ZHATIKOV P.A. - Interaction of human leukocyte elastase with plasma fibronectin and its inhibition by soybean Bowman-Birk protease inhibitor. - Biochemistry (Moscow), 63, 1 9, 1263-1268, 1998.
31. FRITZ H. - Proteinase inhibitors in severe inflammatory processes (septic shock and experimental endotoxaemia): biochemical, pathophysiological and therapeutic aspects. - In: Protein Degradation in Health and Disease (Ciba Foundation Symposium 75), Excerpta Medica, Amsterdam, Oxford, New York, 1980, pp.351-379.
32. BERNKOP-SCHNÜRCH A. - The use of inhibitory agents to overcome the enzymatic barrier to perorally administered therapeutic peptides and proteins. - J.Control. Release, 52, 1-16, 1998.
33. YAMAMOTO A., TANIGUCHI T., RIKYUU K.,TSUJI T., FUJITA T., MURAKAMI M. and MURANISHI S. - Effects of various protease inhibitors on the intestinal absorption and degradation of insulin in rats. - Pharm. Res., 11, 1496-1500, 1994.
34. USHIROGAWA Y., NAKAHIGASHI Y., KIRIYAMA A. and TAKADA K. - Effect of organic acids, trypsin inhibitors and dietary protein on the pharmacological activity of recombinant human granulocyte colony-stimulating factor (rhG-CSF) in rats. -Int. J. Pharm., 81, 133-141, 1992.
35. KENNEDY A.R. - In vitro studies of anticarcinogenic protease inhibitors. - In: Protease Inhibitors as Cancer Chemopreventive Agents, W. Troll and A.R. Kennedy Eds., Plenum Press, New York and London, 1993, pp.65-91.
36. KENNEDY A.R. - Overview: anticarcinogenic activity of proteinase inhibitors. - In: Protease Inhibitors as Cancer Chemopreventive Agents, W. Troll and A.R. Kennedy Eds., Plenum Press, New York and London, 1993, pp. 9-64.
37. YAVELOW J., FINLAY T.H., KENNEDY A.R. and TROLL W. -Bowman-Birk soybean protease inhibitor as an anticarcinogen. -Cancer Res., 43, 2454-2459, 1983.
38. FRITZ H., OPPITZ K.H., MECKL D., KEMKES B., HAENDLE H., SCHULT H. and WERLE E. - Verteilung und Ausscheidung von naturlich vorkommenden und chemisch modifizierten Proteaseinhibitoren nach intravenoser Injektion bei Ratte, Hund (und Mensch). - Hoppe-Seyler's Z. Physiol. Chem., 350, 1541-1550, 1969.
39. PERSIANI S., YEUNG A., SHEN W.C. and KENNEDY A.R. -Polylysine conjugates of Bowman-Birk protease inhibitor as targeted anticarcinogenic agents. - Carcinogenesis, 12, 1149-1152, 1991.
40. BILLINGS P.S., ST. CLAIR W.H., MAKI P.A. and KENNEDY A.R. - Distribution of the Bowman-Birk protease inhibitor in mice following oral administration. - Cancer Lett., 62, 191-197, 1992.
41. LARIONOVA N.I. and TORCHILIN V.P. - Pharmaceutical applications of immobilized proteins. - In: Introduction to Applied Enzymology, Moscow State University Press, Moscow, 1982, pp.284-305.
42. DUNCAN R. - Selective endocytosis of macromolecular drug carriers. - In: Controlled Drug Delivery, J.R. Robinson and V.H.L. Lee Eds., Marcel Dekker, Inc., New York, 1987, pp. 581-621.
43. DEISENHOFER J. and STEIGEMANN W. - The model of the basic pancreatic trypsin inhibitor refined at 1.5 è resolution. In: Proteinase Inhibitors - Bayer Symposium V, H. Fritz, H. Tschesche, L.J. Greene and E. Truscheit Eds., Springer-Verlag, Berlin, 1974, pp. 484-496.
44. SAKHAROV I.YU., LARIONOVA N.I., KAZANSKAYA N.F. and BEREZIN I.V. - Stabilization of proteins by modification with water-soluble polysaccharides. - Enzyme Microb. Technol., 6, 27-30, 1984.
45. LARIONOVA N.I., BALABUSHEVITCH N.G., GLADYSHEVA I.P., MOROZ N.A., KAZANSKAYA N.F, POLEKHINA O.V. and DONETSKY I.A. - Natural proteinase inhibitors as a source of new drugs. - Voprosi Med. Chem., 13, 25-31, 1994 (in Russian).
46. LARIONOVA N.I., KAZANSKAYA N.F. and SAKHAROV I.Y. -Soluble high-molecular weight derivatives of pancreatic inhibitor. Kinetic and thermodynamic study of inhibitor bound to matrices with different charges. - Biochemistry (Moscow), 44, 279-285, 1979.
47. LARIONOVA N.I., KAZANSKAYA N.F., SAKHAROV I.Yu. and MITYUSHINA G.V. - Soluble high-molecular weight derivatives of pancreatic inhibitor. Modification of pancreatic trypsin inhibitor by soluble polysaccharides activated by titanium tetrachloride. -Biochemistry (Moscow), 45, 527-530, 1980.
48. LARIONOVA N.I., KAZANSKAYA N.F. and SAKHAROV I.Y. -Soluble high-molecular weight derivatives of pancreatic inhibitor. Preparation and properties of the pancreatic inhibitor bound to dextran. - Biochemistry (Moscow), 42, 963-968, 1977.
49. LARIONOVA N.I., KAZANSKAYA N.F. and SAKHAROV I.Y. -Soluble high-molecular weight derivatives of pancreatic inhibitor. Preparation and properties of the pancreatic inhibitor bound to carboxymethylcellulose and diethylaminoethyldextran. -Biochemistry (Moscow), 43, 699-703, 1978.
50. LARIONOVA N.I., UNKSOVA L.Y., MIRONOV V.A., SAKHAROV I.Y., KAZANSKAYA N.F. and BEREZIN I.V. -Study of the complex formation between soluble carboxymethyl ethers of polysaccharides and proteins. - Visokomolekulyarnye soedineniya, 23A, 1823-1829, 1981 (in Russian).
51. LARIONOVA N.I., KAZANSKAYA N.F., SAKHAROV I.Y. and BEREZIN I.V. - Soluble high-molecular weight derivatives of pancreatic inhibitor. Influence of electrostatic interaction of the protein with the carrier on the process of their covalent bonding and certain physicochemical characteristics of modified preparations of the pancreatic inhibitor. - Biochemistry (Moscow), 44, 1610-1614, 1979.
52. LARIONOVA N.I., KAZANSKAYA N.F. and MITYUSHINA G.V. -Modified and immobilized preparations of basic polyvalent inhibitor of proteases designed for potential medical use. -Voprosi Med. Khimii, 4, 25-30, 1985 (in Russian).
53. LARIONOVA N.I., MOROZ N.A., BALABUSHEVITCH N.G., KIL'DEEVA N.R. and KHROMOV G.L. - Enzyme engineering approaches to the designing of polymeric forms of aprotinin. -Moscow University Chemistry Bulletin, 50, 25-29, 1995.
54. MOROZ N.A. - Polymeric prolonged aprotinin dosage forms. -PhD Thesis., Moscow State University, 1998.
55. SAKHAROV I.Y., LARIONOVA N.I., MITYUSHINA G.V., GAVRILOVA E.M., KAZANSKAYA N.F. and BEREZIN I.V. - A new technique for preparation of protein-water soluble polymer-antibody conjugates. - Bioorg.Chem., 7, 460-462, 1981.
56. LARIONOVA N.I., MITYUSHINA G.V., KAZANSKAYA N.F., BLIDCHENKO Y.A. and BEREZIN I.V. - Carbohydrate-containing derivatives of the trypsin-kallikrein inhibitor aprotinin from bovine organs. II. Inhibitor coupled to the (carboxymethyl) dextran derivatives of D-galactose. - Biol. Chem. Hoppe-Seyler. 366, 743-748, 1985.
57. LARIONOVA N.I., MITYUSHINA G.V., KAZANSKAYA N.F., BLIDCHENKO Y.A. and BEREZIN I.V. - Carbohydrate-containing derivatives of the trypsin-kallikrein inhibitor aprotinin from bovine organs. I. Modification with lactose, characterization and behaviour of the preparation in vivo. - Hoppe-Seyler's Z. Physiol. Chem., 365, 791-797, 1984.
58. ZABORSKY O.R. - Immobilized Enzymes. - CRC, Cleveland, Ohio, 1973.
59. HUBER R., KUKLA D., BODE W., SCHWAGER P., BARTELS K., DEISENHOFER J. and STEIGEMANN W. - Structure of the complex formed by bovine trypsin and bovine pancreatic trypsin inhibitor. II. Crystallographic refinement at 1.9x resolution. - J. Mol. Biol., 89, 73-101, 1974.
60. ASHWELL. G. and MORELL A.G. - The role of surface carbohydrates in the hepatic recognition and transport of circulating glycoproteins. - Adv. Enzymol. Relat. Areas Mol. Biol., 41, 99-128, 1974.
61. TORCHILIN V.P., MAZAEV A.V., ILINA E.V., GOLDMACHER V.S., SMIRNOV V.N. and CHAZOV E.I. - Chemical aspects of enzyme stabilization and modification for use in therapy. - In: Enzyme Engineering: Future Directions, L.B. Wingard Jr., I. Berezin and A.A. Klyosov Eds., Plenum Press, New York and London, 1980, pp. 219-240.
62. DELGADO C., FRANCIS G.E. and FISHER D. - The uses and properties of PEG-linked proteins. - Crit. Rev. Ther. Drug Carrier Sys., 9, 249-304, 1992.
63. HAVRANOVA M., CECHOVA D., SAUDEK V., METALOVA M. and DROBNIK J. - A high-molecular mass derivative of trypsin-kallikrein inhibitor for potential medical use. II. Study of inhibitory activity. - Hoppe-Seyler's Z. Physiol. Chem., 363, 295-303, 1982.
64. LARIONOVA N.I., MAKEVNINA L.G., NARTIKOVA V.F. and PASKHINA T.S. - Influence of covalent binding of aprotinin to a polysaccharide carrier on its inhibition of human plasma kallikrein, porcine pancreatic kallikrein and trypsin. - Biochemistry (Moscow), 52, 709-715, 1987.
65. CHEN Z. and BODE W. - Refined 2.5x X-ray crystal structure of the complex formed by porcine kallikrein A and the bovine pancreatic trypsin inhibitor. Crystallization, Patterson search, structure determination, refinement, structure and comparison with its components and with the bovine trypsin-pancreatic trypsin inhibitor complex. - J.Mol. Biol., 164, 283-311, 1983.
66. FRITZ H. - Glandular and plant kallikrein inhibitors. - In: Chemistry and Biology of the Kallikrein-Kinin-System in Health and Disease, J.J. Pisano, K.F. Austen Eds., U.S.Government Printing Office, Washington, D.C., 1976, pp. 181-193.
67. LARIONOVA N.I. - Native and immobilized protein proteinase inhibitors for biomedical applications. - PhD Thesis, Moscow State University, 1991.
68. LARIONOVA N.I., MOROZ N.A., BALABUSHEVITCH N.G. and POLEKHINA O.V. - Carbohydrate-containing derivatives of aprotinin for receptor-mediated targeting and longevity. -23rd Intern. Symp. Control. Rel. Bioact. Mater., Kyoto, 7/10 July 1996, pp. 879-880.
69. NISHIKAWA M., YAMASHITA F., TAKAKURA Y. and HASHIDA M. - Molecular design of protein derivatives targeted to the liver via the asialoglycoprotein receptor. - 22nd Int. Symp. Control. Rel. Bioact. Mater., Seattle, 30 July/2 August 1995, pp. 502-503.
70. LARIONOVA N.I., VARTANOV S.S., SOROKINA N.V., GLADYSHEVA I.P. and VARFOLOMEYEV S.D. - Conjugation of the Bowman-Birk soybean proteinase inhibitor with hydroxyethylstarch. - Appl. Biochem. Biotechnol., 62, 175-182, 1997.
71. LARIONOVA N.I., GLADYSHEVA I.P., TOPCHIEVA I.N. and KAZANSKAYA N.F. - Conjugation of the classical Bowman-Birk soybean inhibitor with a copolymer of ethylene oxide and propylene oxide. - Biochemistry (Moscow), 58, 1658-1664, 1993.
72. LARIONOVA N.I., TOPCHIEVA I.N., GLADYSHEVA I.P. and KENNEDY A.R. - The Bowman-Birk inhibitor-polyester conjugate: synthesis, inhibition of serine proteinases, and suppression of radiation transformation in vitro. - Cancer J., 7, 158-163, 1994.
73. TOPCHIEVA I.N., EROKHIN V.N., OSIPOVA S.V., KHTUTSKAYA M.M., KUPRIYANOVA T.A. and BYKOVSKAYA S.N. - Block copolymers of ethylene oxide and propylene oxide (pluronic) as immunomodulators and antitumor agents. - Biomed. Sci., 2, 38-44, 1991.
74. YAVELOW J., COLLINS M., BIRK Y., TROLL W. and KENNEDY A. R. - Nanomolar concentrations of Bowman-Birk soybean protease inhibitor suppres X-ray induced transformation in vitro. - Proc. Natl. Acad. Sci. USA, 82, 5395-5399, 1985.
75. VOSS R.H., ERMLER U., ESSEN L.O., WENZL G., KIM Y.M and FLECKER P. - Crystal structure of the bifunctional soybean Bowman-Birk inhibitor at 0.28-nm resolution: structural peculiarities in a folded protein conformation. - Eur. J. Biochem., 242, 122-131, 1996.
76. GLADYSHEVA I.P., POLEKHINA O.V., SHEN W.C., SHEVCHENKO A.A., KAZANSKAYA N.F. and LARIONOVA N.I. - Structure and biological properties of Bowman-Birk soybean proteinase inhibitor conjugate with block copolymer of ethylene oxide and propylene oxide. - Biochemistry (Moscow), 60, 385-391, 1995.
77. BERNKOP-SCHNÜRCH A. and GOCKEL N.C. - Development and analysis of a polymer protecting from luminal enzymatic degradation caused by α-chymotrypsin. - Drug Dev. Ind. Pharm., 23, 733-740, 1997.
78. BERNKOP-SCHNÜRCH A., KRAULAND A. and VALENTA C. - Development of drug delivery systems with protective effect towards Gl-proteinases. - 2nd World Meeting on Pharmaceutics, Biopharmaceutics, Pharmaceutical Technology, Paris, 25/28 May 1998, APGI/APV Ed., pp. 989-990.
79. EKRAMI H., KENNEDY A.R., WITSCHI H.W. and SHEN W.C. -Cationized Bowman-Birk protease inhibitor as a targeted cancer chemopreventive agent. - J. Drug Targeting, 1, 41 -49, 1993.
80. LIN J.Y., LI J.S. and TUNG T.C. - Lectin derivatives of methotrexate and chlorambucil as chemotherapeutic agents. -J. Natl. Cancer Inst., 66, 523-528, 1981.
81. LIN J.Y. and HU M.N. - The Con A conjugate of Bowman-Birk soybean trypsin inhibitor as an anticarcinogen. - In: Lectins: Biology, Biochemistry, Clinical Biochemistry, T.C. Bog-Hansen, E. van Driessche Eds., Walter de Gruyter, Berlin, New York, 1986, pp. 409-416.
82. LIN J. Y., LIN L.L. - Antitumor lectin-trypsin inhibitor conjugate. -J. Natl. Cancer Inst., 74, 1031-1036, 1985.
83. LIN J.Y., HSIEH Y.S. and CHU S.C. - Chimeric protein: abrin B chain-trypsin inhibitor conjugate as a new antitumor agent. -Biochem. Internal., 19, 313-323, 1989.
84. YAVELOW J., CAGGANA M. and BECK K.A. - Proteases occurring in the cell membrane: a possible cell receptor for the Bowman-Birk type of protease inhibitors. - Cancer Res., 47, 1598-1601, 1987.
85. YAVELOW J., SCOTT C.B. and MAYER T.C. - Fluorescent visualization of binding and internalization of the anticarcinogenic Bowman-Birk type protease inhibitors in transformed fibroblasts. - Cancer Res., 47, 1602-1607, 1987.
86. LIN J.Y., KAO W.Y., TSERNG K.Y., CHEN C.C. and TUNG T.C. - Effect of crystalline abrin on the biosynthesis of protein, RNA and DNA in experimental tumors. - Cancer Res., 30, 2431-2433, 1970.
87. LARIONOVA N.I., GLADYSHEVA I.P., POLEKHINA O.V., KUROCHKINA L.P. and GORBATOVA E.N. - Synthesis and biodistribution of Bowman-Birk soybean protease inhibitor conjugate with amphiphilic polyester. - Appl. Biochem. Biotech., 61, 139-148, 1996.
88. TYURINA O.P., MALYKH E.V., BALABUSHEVICH N.G. and LARIONOVA N.I. - The modification of basic pancreatic trypsin inhibitor by fatty acid derivatives. - Bioorg. Chem. (Moscow), 24, 301-304, 1998.
89. LARIONOVA N.I., MALYKH E.V., TIOURINA O.P. and SHEN W.C. - Fatty acid derivatives of protein protease inhibitors. - 24th Intern. Symp. Control. Rel. Bioact. Mater., Las Vegas, 21/26 June 1998, pp. 483-484.
90. MALYKH E.V., TYURINA O.P., BALABUSHEVITCH N.G. and LARIONOVA N.I. - The influence of bovine pancreatic trypsin inhibitor hydrophobization on ability to inhibit bovine trypsin and human leukocyte elastase. - Biochemistry (Moscow), 63, 1317-1319, 1998.
91. EKRAMI H.M., KENNEDY A.R. and SHEN W.C. - Water-soluble fatty and derivatives as acylating agents for reversible lipidization of polypeptides. - FEBS Lett., 371, 283-286, 1995.
92. LARIONOVA N.I., SAKHAROV I.Yu., KAZANSKAYA N.F., ZHURAVLYOV A.G., VLADIMIROV V.G. and TOLSTICH P.I. -Modification of trypsin pancreatic inhibitor by polysaccharides for prolongation of therapeutic effect. - In: Enzyme Engineering: Future Directions, L.B. Wingard, Jr., I. Berezin and A.A. Klyosov Eds., Plenum Press, New York and London, 1980, pp. 241-255.
93. GOSTISCHEV V.K., TOLSTYKH P.I., VASILKOVA Z.F., VLASOV L.G., VIRNIK A.D., LARIONOVA N.I. and RYLTSEV V.V. - Experimental and clinical use of various forms of immobilized proteinases and their inhibitors. - Voprosi Med. Khimii, 14, 21-24, 1985.
94. WITSCHI H. and KENNEDY A.R. - Modulation of lung tumour development in mice by dietary addition of the Bowman-Birk protease inhibitor. - Carcinogenesis, 10, 2275-2277, 1989.