Functional analysis of human cardiac troponin by the in vitro motility assay: comparison of adult, foetal and failing hearts

Cardiovascular Research

Volumn 43, Issue 4, 1999, Pages 884-891

  Purcell, Ian F ^a   Bing, Wu ^a   Marston, Steven B ^a  

^a IMPERIAL COLLEGE SCHOOL OF MEDICINE   (United Kingdom)

Author keywords

Cardiomyopathy; Contractile apparatus; Contractile function; Developmental biology; Heart failure

Indexed keywords

TROPOMYOSIN; TROPONIN;

ADULT; ARTICLE; ASSAY; CARDIOMYOPATHY; CONTROLLED STUDY; FETUS; HEART DEVELOPMENT; HEART FAILURE; HUMAN; HUMAN TISSUE; IN VITRO STUDY; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PHOSPHORYLATION;

ACTINS; ADULT; ANIMALS; BIOLOGICAL ASSAY; BLOTTING, WESTERN; CALCIUM; ELECTROPHORESIS, POLYACRYLAMIDE GEL; FETAL HEART; HEART FAILURE, CONGESTIVE; HUMANS; IMMUNOBLOTTING; MUSCLE CONTRACTION; MYOCARDIAL CONTRACTION; RABBITS; TROPOMYOSIN; TROPONIN; TROPONIN C; TROPONIN I; TROPONIN T;

EID: 0032830884     PISSN: 00086363     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0008-6363(99)00123-6     Document Type: Article

References (34)

1. Schiaffino S., Reggiani C. Molecular diversity of myofibrillar proteins: gene regulation and functional significance. Physiol Rev. 76:1996;371-423.
2. Anderson P.A.W., Malouf N.N., Oakeley A.E., Pagani E.D., Allen P.D. Troponin T isoform expression in humans. A comparison among normal and failing heart, fetal heart and adult and fetal skeletal muscle. Circ Res. 69:1991;1226-1233.
3. Hunkeler N.M., Kullman J., Murphy A.M. Troponin I isoform expression in the human heart. Circ Res. 69:1991;1409-1414.
4. Sasse S., Brand N., Kyprianou P., et al. Troponin I gene expression during human cardiac development and in end-stage heart failure. Circ Res. 72:1993;932-938.
5. Anderson P., Greig A., Mark T., et al. Molecular basis of human cardiac troponin T isoforms expressed in the developing, adult, and failing heart. Circ Res. 76:1995;681-686.
6. Mesnard L., Logeart D., Taviaux S., et al. Human cardiac troponin T: cloning and expression of new isoforms in the normal and failing heart. Circ Res. 76:1995;687-692.
7. Townsend P.J., Barton P.J.R., Yacoub M.H., Farza H. Molecular cloning of human cardiac troponin T isoforms: expression in developing and failing heart. J Mol Cell Cardiol. 27:1995;2223-2236.
8. Saba Z., Nassar R., Ungerleider R., Oakeley A., Anderson P. Cardiac troponin T isoform expression correlates with pathophysiological descriptors in patients who underwent corrective surgery for congenital heart disease. Circulation. 94:1996;472-476.
9. Mesnard-Rouiller L., Mercadier J.-J., Butler-Brown G., et al. Troponin T mRNA and protein isoforms in the human left ventricle: pattern of expression in failing and control hearts. J Mol Cell Cardiol. 29:1997;3043-3055.
10. Bodor G.S., Oakeley A.E., Allen P.D., et al. Troponin I phosphorylation in normal and failing adult human heart. Circulation. 96:1997;1495-1500.
11. Wolff M., Buck S., Stoker S., Greaser M., Mentzer R. Myofibrillar calcium sensitivity of isometric tension is increased in human dilated cardiomyopathies. J Clin Invest. 98:1996;167-176.
12. Tobacman L.S., Lee R.S. Isolation and functional comparison of bovine cardiac troponin T isoforms. J Biol Chem. 262:1987;4059-4064.
13. Tobacman L.S. Structure-function studies of the amino-terminal region of bovine cardiac troponin T. J Biol Chem. 263:1988;2668-2672.
14. Malhotra A. Regulatory proteins in hamster cardiomyopathy. Circ Res. 66:1990;1302-1309.
15. Malhotra A., Scheuer J. Troponin-tropomyosin abnormalities in hamster cardiomyopathy. J Clin Invest. 86:1990;286-292.
16. 2+ binding associated with troponin T isoform switching in developing rabbit heart. Circ Res. 66:1990;1204-1216.
17. Nassar R., Malouf N., Kelly M., Oakeley A., Anderson P. Force-pCa relation and troponin T isoforms of rabbit myocardium. Circ Res. 69:1991;1470-1475.
18. Schiaffino S., Gorza L., Ausini S. Troponin isoform switching in the developing heart and its functional properties. Trends Cardiovasc Med. 3:1993;12-17.
19. 2+ sensitivity of skinned cardiac muscle contractility coincident with troponin-T band shifts in the diabetic rat. Circ Res. 76:1995;600-606.
20. 2+: the thin filament is switched as a single cooperative unit. J Biol Chem. 270:1995;7836-7841.
21. 2+ regulation of thin filament motility. Biochem Biophys Res Commun. 236:1997;760-764.
22. Margossian S.S., Lowey S. Preparation of myosin and its subfragments from rabbit skeletal muscle. Methods Enzymol. 85:1982;55-71.
23. Potter J.D. Preparation of troponin and its subunits. Methods Enzymol. 85:1982;241-263.
24. 2+-dependant effects on actin-tropomyosin filament motility. Biochem J. 327:1997;335-340.
25. Kron S.J., Toyoshima Y.Y., Uyeda T.Q.P., Spudich J.A. Assays for actin sliding movement over myosin coated surfaces. Methods Enzymol. 196:1991;399-416.
26. Marston S.B., Fraser I.D.C., Wu B., Roper G. A simple method for automatic tracking of actin filaments in the motility assay. J Muscle Res Cell Motil. 17:1996;497-506.
27. Wolff M.R., McDonald K.S., Moss R.L. Rate of tension development in cardiac muscle varies with level of activator calcium. Circ Res. 76:1995;154-160.
28. 2+ regulation of actin-tropomyosin-troponin [abstract]. J Mol Cell Cardiol. 30:1998;A172.
29. Pagani E., Alousi A., Grant A., et al. Changes in myofibrillar content and Mg-ATPase activity in ventricular tissues from patients with heart failure caused by coronary artery disease, cardiomyopathy, or mitral valve insufficiency. Circ Res. 63:1988;380-385.
30. Solaro J., Powers F., Gao L., Gwathmey J. Control of myofilament activation in heart failure. Circulation. 87:(suppl VII):1993;VII-38-VII-43.
31. Harding S., Brown L., Wynne D., Davies C., Poole-Wilson P. Mechanisns of β-adrenoceptor desensitisation in the failing human heart. Cardiovasc Res. 28:1994;1451-1460.
32. Hasenfuss G., Meyer M., Schillinger W., et al. Calcium handling proteins in the failing human heart. Basic Res Cardiol. 92:(suppl 1):1997;87-93.
33. Lin D., Bobkova A., Homsher E., Tobacman L.S. Altered cardiac troponin T in vitro function in the presence of a mutation implicated in famial hypertrophic cardiomyopathy. J Clin Invest. 97:1996;2842-2848.
34. Watkins H., McKenna W., Thierfelder L., et al. Mutations in the genes for cardiac troponin T and α-tropomyosin in hypertrophic cardiomyopathy. N Engl J Med. 332:1995;1058-1064.