Purification of ecdysone oxidase and 3-dehydroecdysone 3α-reductase from the cotton leafworm, Spodoptera littoralis

Insect Biochemistry and Molecular Biology

Volumn 29, Issue 10, 1999, Pages 899-908

  Chen, Jian Hua ^a   Powls, Roy ^a   Rees, Huw H ^a   Wilkinson, Mark C ^a  

^a UNIVERSITY OF LIVERPOOL   (United Kingdom)

Author keywords

3DE 3 reductase; Ecdysone oxidase; Spodoptera littoralis

Indexed keywords

ECDYSONE; ECDYSTEROID; MONOMER; OXIDOREDUCTASE; STEROID REDUCTASE;

ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; CYTOSOL; ENZYME LOCALIZATION; ENZYME PURIFICATION; ENZYME SPECIFICITY; GEL CHROMATOGRAPHY; GEL FILTRATION; HYDROPHOBICITY; LEPIDOPTERA; MIDGUT; MOLECULAR WEIGHT; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; TISSUE DISTRIBUTION;

SPODOPTERA LITTORALIS;

EID: 0032829497     PISSN: 09651748     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0965-1748(99)00065-X     Document Type: Article

References (32)

1. Blackford M.J.P., Clarke B.S., Dinan L. Distribution and metabolism of exogenous ecdysteroids in the Egyptian cotton leafworm Spodoptera littoralis (Lepidoptera: Noctuidae). Arch. Insect Biochem. Physiol. 34:1997;329-346.
2. Blais C., Lafont R. Ecdysteroid metabolism by soluble enzymes from an insect - metabolic relationships between 3-beta-hydroxyecdysteroids, 3-alpha-hydroxyecdysteroids and 3-oxoecdysteroids. Hoppe-Seylers Z. Physiol. Chem. 365:1984;809-817.
3. Bradford M.M. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:1976;248-254.
4. Chen J.-H., Turner P.C., Rees H.H. Molecular cloning and characterisation of haemolymph 3-dehydroecdysone 3β-reductase from the cotton leafworm, Spodoptera littoralis. A new member of the third superfamily of oxidoreductases. J. Biol. Chem. 274:1999;10551-10556.
5. Chen J.-H., Kabbouh M., Fisher M.J., Rees H.H. Induction of an inactivation pathway for ecdysteroids in larvae of the cotton leafworm, Spodoptera littoralis. Biochem. J. 301:1994;89-95.
6. Chen J.-H., Webb T.J., Powls R., Rees H.H. Purification and characterisation of haemolymph 3-dehydroecdysone 3β-reductase in relation to ecdysteroid biosynthesis in the cotton leafworm Spodoptera littoralis. Eur. J. Biochem. 242:1996;394-401.
7. Chen J.-H., Powls R., Rees H.H. Purification of the enzymes of ecdysteroid 3-epimerization from the midgut of the cotton leafworm, Spodoptera littoralis. Biochem. Soc. Trans. 24:1996;436S.
8. Gilbert L.I. The endocrine control of molting: the tobacco hornworm, Manduca sexta, as a model system. Koolman J. Ecdysone: From Chemistry to Mode of Action. 1989;448-471 Georg Thieme-Verlag, Stuttgart.
9. Heukeshoven J., Dernick R. Simplified method for silver staining of proteins in polyacrylamide gels and mechanism of silver staining. Electrophoresis. 6:1985;103-112.
10. Kiriishi S., Rountree D.B., Sakurai S., Gilbert L.I. Prothoracic gland synthesis of 3-dehydroecdysone and its hemolymph 3β-reductase mediated conversion to ecdysone in representative insects. Experientia. 46:1990;716-722.
11. Kubo K. Elimination of protein band "edge tailing" in Weber-Osborn-type slab gel electrophoresis by glycerol. Anal. Biochem. 213:1993;200-205.
12. Koolman J. Ecdysone oxidase in insects. Hoppe-Seylers Z. Physiol. Chem. 359:1978;1315-1321.
13. Koolman J. Ecdysone oxidase. Law J.H., Rilling H.C. Methods in Enzymology, vol. 111. 1985;419-429 Academic Press, Inc.
14. Koolman J. Ecdysone: From Chemistry to Mode of Action. 1989;Georg Thieme-Verlag, Stuttgart.
15. Koolman J., Karlson P. Ecdysone oxidase, an enzyme from the blowfly Calliphora erythrocephala (Meigen). Hoppe-Seylers Z. Physiol. Chem. 356:1975;1131-1138.
16. Koolman J., Karlson P. Ecdysone oxidase: reaction and specificity. Eur. J. Biochem. 89:1978;453-460.
17. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 227:1970;680-685.
18. Lafont R., Connat J.-L. Pathways of ecdysone metabolism. Koolman J. Ecdysone: From Chemistry to Mode of Action. 1989;167-173 Georg Thieme-Verlag, Stuttgart.
19. Lafont R., Koolman J. Ecdysone metabolism. Hoffmann, Porchet P. Biosynthesis, Metabolism and Mode of Action of Invertebrate Hormones. 1984;196-226 Springer, Berlin.
20. Lehmann M., Koolman J. Regulation of ecdysone metabolism. Koolman J. Ecdysone: From Chemistry to Mode of Action. 1989;217-220 Georg Thieme-Verlag, Stuttgart.
21. Mayer R.T., Durrant J.L., Holman G.M., Weirich G.F., Svoboda J.A. Ecdysone 3-epimerase from the midgut of Manduca sexta (L). Steroids. 34:1979;555-562.
22. Milner N.P., Rees H.H. Involvement of 3-dehydroecdysone in the 3-epimerization of ecdysone. Biochem. J. 231:1985;369-374.
23. Rees H.H. Ecdysteroid biosynthesis and inactivation in relation to function. Eur. J. Entomol. 92:1995;9-39.
24. Rees H.H., Isaac R.E. Biosynthesis of ovarian ecdysteroid phosphates and their metabolic fate during embryogenesis in Schistocerca gragaria. Hoffmann J., Porchet P. Biosynthesis, Metabolism and Mode of Action of Invertebrate Hormones. 1984;181-195 Springer, Berlin.
25. Sakurai S., Warren J.T., Gilbert L.I. Mediation of ecdysone synthesis in Manduca sexta by a hemolymph enzyme. Arch. Insect Biochem. Physiol. 10:1989;179-197.
26. Warren J.T., Sakurai S., Rountree K.B., Gilbert L.I., Lee S.S., Hakanishi K. Regulation of the ecdysteroid titer of Manduca sexta: Reappraisal of the role of the prothoracic glands. Proc. Natl. Acad. Sci. USA. 85:1988;958-962.
27. Webb T.J., Powls R., Rees H.H. Enzymes of ecdysteroid transformation and inactivation in the midgut of the cotton leafworm, Spodoptera littoralis: properties and developmental profiles. Biochem. J. 312:1995;561-568.
28. Webb T.J., Powls R., Rees H.H. Characterization, fractionation and kinetic properties of the enzymes of ecdysteroid 3-epimerization and phosphorylation isolated from the midgut cytosol of the cotton leafworm, Spodoptera littoralis. Insect Biochem. Mol. Biol. 26:1996;809-816.
29. Weirich G.F. Enzymes involved in ecdysone metabolism. Koolman J. Ecdysone: From Chemistry to Mode of Action. 1989;174-180 Georg Thieme-Verlag, Stuttgart.
30. Weirich G.F., Svoboda J.A. 3-Oxoecdysteroid reductases in Manduca sexta midgut. Arch. Insect Biochem. Physiol. 21:1992;91-102.
31. Williams D.R., Chen J.-H., Fisher M.J., Rees H.H. Induction of enzymes involved in molting hormone (ecdysteroid) inactivation by ecdysteroids and an agonist, 1,2-dibenzoyl-1-tert-butylhydrazine (RH-5849). J. Biol. Chem. 272:1997;8427-8432.
32. Zhang M.L., Kubo I. Metabolic fate of ecdysteroids in larval Bombyx mori and Heliothis virescens. Insect Biochem. Mol. Biol. 23:1993;831-843.