Somatic and germinal inheritance of an FLP-mediated deletion in transgenic tobacco

Journal of Experimental Botany

Volumn 50, Issue 338, 1999, Pages 1447-1456

  Davies, Gareth J ^b   Kilby, Nigel J ^a,b   Riou Khamlichi, Catherine ^b   Murray, James A M ^b  

^a Lab Plant Physiol Molec Biol B   (Finland)

^b UNIVERSITY OF CAMBRIDGE   (United Kingdom)

Author keywords

FLP recombinase; Gene activation; Inducible gene expression; Site specific recombination; Tobacco

Indexed keywords

GENETIC ENGINEERING; INHERITANCE; PLANT GENETICS; RECOMBINASE; TRANSGENIC PLANT;

EMBRYOPHYTA; NICOTIANA TABACUM; TABACUM;

EID: 0032828099     PISSN: 00220957     EISSN: None     Source Type: Journal    
DOI: 10.1093/jxb/50.338.1447     Document Type: Article

References (27)

1. Araki H, Jearnpipatkul A, Tatsumi H, Sakurai T, Ushio K, Muta T, Oshima Y. 1985. Molecular and functional organisation of the yeast plasmid pSR1. Journal of Molecular Biology 182, 191-203.
2. Austin S, Ziese M, Sternberg N. 1981. A novel role for site-specific recombination in maintenance of bacterial replicons. Cell 25, 729-736.
3. Bar M, Lesham B, Gilboa N, Gidoni D. 1996. Visual characterisation of recombination at FRT-gusA loci in transgenic tobacco mediated by constitutive expression of the native FLP recombinase. Theoretical and Applied Genetics 93, 407-413.
4. Bayley CC, Morgan M, Dale EC, Ow DW. 1992. Exchange of gene activity in transgenic plants catalyzed by the Cre-lox site-specific recombination system. Plant Moleculcar Biology 18, 353-361.
5. Broach JR, Hicks JB. 1980. Replication and recombination functions associated with the yeast plasmid 2μ circle. Cell 21, 501-508.
6. Chinaut AC, Blakesley VA, Roessler E, Willis DG, Smith CA, Cook RG, Fenwick Jr RG. 1986. Characterisation of transferable plasmids from Shigella flexneri 2a that confer resistance to trimethoprim, streptomycin and sulfonomides. Plasmid 15, 119-131.
7. Dale EC, Ow DW. 1991. Gene transfer with subsequent removal of the selection gene from the host genome. Proceedings of the National Academy of Sciences, USA 88, 10558-10562.
8. Eady C, Lindsey K, Twell D. 1994. Differential activation and conserved vegetative cell-specific activity of a late pollen promoter in species with bicellular and tricellular pollen. The Plant Journal 5, 543-550.
9. Gleave AP. 1992. A versatile binary vector system with a T-DNA organisational structure conducive to efficient integration of cloned DNA into the plant genome. Plant Molecular Biology 20, 1203-1207.
10. Horsch RB, Fry JE, Hoffman NL, Eichholtz D, Rogers SG, Fraley RT. 1985. A simple and general method of transferring genes into plants. Science 277, 1229-1231.
11. Jefferson RA. 1987. Assaying chimeric genes in plants: the GUS gene fusion system. Plant Molecular Biology Report 5, 387-405.
12. Kilby NJ, Davies GJ, Snaith MR, Murray JAH. 1995. FLP recombinase in transgenic plants: constitutive activity in stably transformed tobacco and generation of marked cell clones in Arabidopsis. The Plant Journal 8, 637-652.
13. Kühn R, Schwenk F, Aguet M, Rajewsky K. 1995. Inducible gene targeting in mice. Science 269, 1427-1249.
14. Lloyd AM, Davis RW. 1994. Functional expression of the yeast FLP/FRT site-specific recombination system in Nicotiana tabacum. Molecular and General Genetics 242, 653-657.
15. Lyznik LA, Mitchell JC, Hirayama L, Hodges TK. 1993. Activity of yeast FLP recombinase in maize and rice protoplasts. Nucleic Acids Research 21, 969-975.
16. Lyznikt LA, Rao KV, Hodges TK. 1996. FLP-mediated recombination of FRT sites in the maize genome. Nucleic Acids Research 24, 3784-3789.
17. Medberry SL, Dale E, Qin M, Ow DW. 1995. Intrachromosomal rearrangements generated by cre-lox site specific recombination. Nucleic Acids Research 23, 485-490.
18. Murashige T, Skoog F. 1962. A revised medium for rapid plant growth and bioassays with tobacco tissue cultures. Physiologia Plantarum 15, 473-497.
19. Odell JT, Caimi P, Sauer P, Russell S. 1990. Site-directed recombination in the genome of transgenic tobacco. Molecular and General Genetics 223, 369-378.
20. Onouchi H, Nishihama R, Kudo M, Machida Y, Machida C. 1995. Visualization of site-specific recombination catalysed by a recombinase from Zygosaccharomyces rouxii in Arabidopsis thaliana. Molecular and General Genetics 247, 653-660.
21. Qin M, Bayley C, Stockton T, Ow DW. 1994. Cre recombinase-mediated site-specific recombination between plant chromosomes Proceedings of the National Academy of Sciences, USA 91, 1706-1710.
22. Rohlmann A, Gotthardt M, Willnow TE, Hammer RE, Herz, J. 1996. Sustained somatic gene inactivation by viral transfer of Cre recombinase. Nature Biotechnology 14, 1562-1565.
23. Snaith MR, Murray JAH, Boulter CA. 1995. Multiple cloning sites containing loxP and FRT recognition sites for the Cre and Flp site-specific recombinases. Gene 166, 173-174.
24. Severin K, Schöffl F. 1990. Heat-inducible hygromycin resistance in transgenic tobacco. Plant and Molecular Biology 15, 827-833.
25. Sonti RV, Tissier AF, Wong D, Viret J-F, Signer ER. 1995. Activity of the yeast FLP recombinase in Arabidopsis. Plant and Molecular Biology 28, 1127-1132.
26. Stuurman J, Nijkamp HJ, van Haaren MJJ. 1998. Molecular insertion-site selectivity of Ds in tomato. The Plant Journal 14, 215-223.
27. Szymkowiak EJ. 1996. What chimeras can tell us about plant development. Annual Review of Plant Physiology and Plant and Molecular Biology 47, 351-376.