Giardia and Cryptosporidium methods for water testing - Current status and future needs

Water Supply

Volumn 17, Issue 2, 1999, Pages 49-55

  Clancy, J L ^a   Fricker, C R ^b  

^a Clancy Environmental Consultants ^*  (United Kingdom)

^b THAMES WATER UTILITIES LTD   (United Kingdom)

Author keywords

Concentration; Cryptosporidium; Detection; Methods; Separation; Viability; Water

Indexed keywords

PROTOZOA; WATER ANALYSIS; WATER QUALITY;

WATER QUALITY MONITORING;

WATER SUPPLY;

BACTERIUM; DETECTION METHOD; WATER QUALITY;

CONFERENCE PAPER; CRYPTOSPORIDIUM; GIARDIA; NONHUMAN; OOCYST; TECHNIQUE; UNITED KINGDOM; WATER ANALYSIS; WATER QUALITY;

EID: 0032827148     PISSN: 07351917     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Conference Paper

References (11)

1. Shepherd, K.M. and Wyn-Jones, A.P. (1996). An evaluation of methods for the simultaneous detection of Cryptosporidium oocysts and Giardia cysts from water. Applied and Environmental Microbiology 62, 1317-1322.
2. Fricker, C.R., Turner N.B., Rolchigo P.M., Margolin A.B. and Crabb J.H. (1995). Improved recovery of Cryptosporidium oocysts from drinking water using vortex-flow filtration combined with immunomagnetic separation. The American Journal of Tropical Medicine and Hygiene. 53, (suppl.) 285.
3. Fricker, C.R. (1995). Detection of Cryptosporidium and Giardia in water. In: Protozoan parasites in water (W.B. Betts, D. Casemore, C.R. Fricker, R. V. Smith and J. Watkins eds). London, The Royal Society of Chemistry, pp.91-96
4. Vesey, G., Slade, J.S. and Fricker, C.R. (1991). Taking the eye strain out of environmental Cryptosporidium analysis. Letters in Applied Microbiology 13, 62-65.
5. Vesey, G., Slade, J.S., Byrne, M., Shepherd, K., Dennis, P.J. and Fricker, C.R. (1993b). Routine monitoring of Cryptosporidium oocysts in water using flow cytometry. Journal of Applied Bacteriology 75, 87-90.
6. Graczyk, T.K., Cranfield, M.R., Fayer, R. (1996a). Evaluation of commercial enzyme immunoassay (EIA) and immunofluorescent antibody (IFA) test kits for detection of Cryptosporidium oocysts of other species other than Cryptosporidium parvum. The American Journal of Tropical Medicine and Hygiene 54, 27-279
7. Johnson, D.W. Pieniazek, N.J., Griffin, D.W., Misener, L. and Rose, J.B. (1995). Development of a PCR protocol for sensitive detection of Cryptosporidium oocysts in water samples. Applied and Environmental Microbiology 61, 3849-3855.
8. Stinear, T., Matusan, A., Hines, K. and Sandery, M. (1996). Detection of a single viable Cryptosporidium parvum oocyst in environmental concentrates by reverse transcription-PCR. Applied and Environmental Microbiology 62, 3385-3390.
9. Campbell, A.T., Robertson, L.J. and Smith, H.V. (1992). Viability of Cryptosporidium parvum oocysts: correlation of in vitro excystation with inclusion or exclusion of fluorogenic vital dyes. Applied and Environmental Microbiology 58, 348-3493.
10. Upton, S.J., Tilley, M. and Brillhart, D.B. 1994 Comparative development of Cryptosporidium parvum (Apicomplexa) in eleven continuous host lines. FEMS Microbiology Letters, 118, 233-236.
11. Rochelle, P.A., Ferguson, D.M., Handojo, T.J., De Leon, R., Stewart, M.H. and Wolfe, R.L. (1996). Development of a rapid detection procedure for Cryptosporidium, using in vitro cell culture combined with PCR. Journal of Eukaryotic Microbiology 43, S72.