Identification of false-positive CBFβ/MYH11 RT-PCR results

Leukemia

Volumn 13, Issue 10, 1999, Pages 1617-1619

  Hackwell, S M ^a   Robinson, D O ^a   Harvey, J F ^a   Ross, F M ^a  

^a SALISBURY DISTRICT HOSPITAL   (United Kingdom)

Author keywords

Acute myeloid leukaemia; CBF MYH11 transcripts; RT PCR

Indexed keywords

DNA;

ACUTE GRANULOCYTIC LEUKEMIA; ADOLESCENT; ADULT; AGED; ARTICLE; CANCER GENETICS; CHILD; CHROMOSOME 16; CHROMOSOME INVERSION; CLINICAL ARTICLE; CONTROLLED STUDY; CYTOGENETICS; HUMAN; HUMAN TISSUE; INTRON; LABORATORY DIAGNOSIS; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA SPLICING; RNA TRANSCRIPTION; SEQUENCE HOMOLOGY;

EID: 0032826948     PISSN: 08876924     EISSN: None     Source Type: Journal    
DOI: 10.1038/sj.leu.2401545     Document Type: Article

References (8)

1. Le Beau MM, Larson RA, Bitter MA, Vardiman JW, Golomb HM, Rowley JD. Association of an inversion of chromosome 16 with abnormal marrow eosinophils in acute myelomonocytic leukemia: a unique cytogenetic-clinicopathological association. New Engl J Med 1983; 309: 630-639.
2. Liu P, Tarlé SA, Hajara A, Claxton DF, Marlton P, Freedman M, Siciliano MJ, Collins FS. Fusion between transcription factor CBFβ/PEB2β and a myosin heavy chain in acute myeloid leukemia. Science 1993; 261: 1041-1044.
3. Claxton DF, Liu P, Hsu HB, Marlton P, Hester J, Collins F, Deisseroth AB, Rowley JD, Siciliano MJ. Detection of fusion transcripts generated by the inversion 16 chromosome in acute myelogeneous leukemia. Blood 1994; 83: 1750-1756.
4. Burnett AK, Goldstone AH, Stevens R, Hann I, Rees JK, Wheatley K. Biological characteristics of disease determine the outcome of allogenic or autologous BMT in AML CR1. Blood 1995; 86: (Suppl. 1): 2452a.
5. Langabeer SE, Walker H, Gale RE, Wheatley K, Burnett AK, Goldstone AH, Linch DC. Frequency of CBFβ/MYH11 fusion transcripts in patients entered into the UK MRC AML trials. Br J Haematol 1997; 96: 726-739.
6. Lion T. Control genes in reverse transcriptase-polymerase chain reaction assays. Leukemia 1996; 10: 1527-1528.
7. Van der Reijden BA, Lombardo M, Dauwerse HG et al. RT-PCR diagnosis of patients with acute nonlymphocytic leukemia and inv(16)(p13q22) and identification of new alternative splicing in CBFβ-MYH11 transcripts. Blood 1995; 86: 277-282.
8. Van der Reijden BA, Hagemeijer A, Breuning MH. Novel CEFβ-MYH11 fusion transcripts or reverse transcription-polymerase chain reaction artifacts? Blood 1996; 87: 2605-2606.