Effects of pH on a fluorogenic vital dyes assay (4',6'-diamidino-2-phenyl-indole and propidium iodide) for Cryptosporidium sp. oocysts

Water Research

Volumn 33, Issue 13, 1999, Pages 3037-3039

  Bukhari, Z ^a   Glen, W G ^a   Clancy, J L ^a  

^a Clancy Environmental Consultants Inc   (United States)

Author keywords

Cryptosporidium; Crystals; DAPI PI; Fluorogenic vital dye assay; Oocysts; pH

Indexed keywords

AROMATIC COMPOUNDS; ASSAYS; CRYSTALLIZATION; DYES; MICROORGANISMS; MICROSCOPIC EXAMINATION; MONOCLONAL ANTIBODIES; SALTS;

CRYPTOSPORIDIUM; DIAMIDINO PHENYL INDOLE; FLUOROGENIC VITAL DYES ASSAY; HANK BALANCE SALT SOLUTION; OOCYSTS; PROPIDIUM IODIDE;

PH EFFECTS;

4',6 DIAMIDINO 2 PHENYLINDOLE; DYE; PROPIDIUM IODIDE;

CELL VIABILITY; CRYPTOSPORIDIUM; CRYSTALLIZATION; ENVIRONMENTAL MONITORING; NONHUMAN; OOCYST; PH; PRIORITY JOURNAL; SHORT SURVEY; STAINING;

CRYPTOSPORIDIUM; CRYPTOSPORIDIUM PARVUM; CRYPTOSPORIDIUM SP.;

CHEMICAL COMPOSITION; COLOR; DYE; FLUORESCENCE;

EID: 0032821546     PISSN: 00431354     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0043-1354(99)00013-5     Document Type: Article

References (14)

1. Black E.K., Finch G.R., Taghi-Kilani R., Belosevic M. Comparison of assays for Cryptosporidium parvum oocysts viability after chemical disinfection. FEMS Microbiol. Lett. 135:1996;187-189.
2. Bukhari Z., Smith H.V. Effect of three concentration techniques on viability of Cryptosporidium parvum oocysts recovered from bovine faeces. J. Clin. Microbiol. 33:1995;2592-2595.
3. Bukhari Z., Smith H.V. Cryptosporidium parvum: oocysts excretion and viability patterns in experimentally infected lambs. Epidemiol. Infect. 119:1997;105-108.
4. Campbell A.T., Robertson L.J., Smith H.V. Viability of Cryptosporidium parvum oocysts: correlation of in vitro excystation with inclusion or exclusion of fluorogenic vital dyes. Appl. Environ. Microbiol. 58:1992;3488-3493.
5. Chappell C.L., Okhuysen P.C., Sterling C.R., Dupont H.L. Cryptosporidium parvum: intensity and infection and oocyst excretion patterns in healthy volunteers. J. Infect. Dis. 173:1996;232-236.
6. Clancy J. L. and Fricker C. R. (1998) Control of Cryptosporidium: how effective is drinking water treatment? J. Int. Assoc. Water Quality July/August, 37-41.
7. Clancy J.L., Gollnitz W.D., Tabib Z. Commercial labs: how accurate are they? J. AWWA. 86:1994;89-97.
8. Craun G.F., Frost F., Calderon R.L., Via S.H. Waterborne outbreaks of cryptosporidiosis. J. AWWA. 90:1998;81-91.
9. DuPont H.L., Chappell C.L., Sterling C.R., Okhuysen P.C., Rose J.B., Jakubowski W. The infectivity of Cryptosporidium parvum in healthy volunteers. N. Engl. J. Med. 332:1995;855-859.
10. Finch G.R., Daniels C.W., Black E.K., Schaefer F.W. III, Belosovic M. Dose response of Cryptosporidium parvum in outbred neonatal CD-1 mice. Appl. Environ. Microbiol. 59:1993;3661-3665.
11. Fricker C.R., Turner N.B., Rolchigo P.M., Margolin A.D., Crabb J.H. Improved recovery of Cryptosporidium oocysts from drinking water using vortex flow filtration combined with immunomagnetic separation. J. Am. Soc. Trop. Med. Hyg. 53:1995;285.
12. Kramer M.H., Herwaldt B.L., Craun G.F., Calderon R.L., Juranek D.D. Waterborne disease: 1993 and 1994. J. AWWA. 88:1996;66-80.
13. Robertson L.J., Campbell A.T., Smith H.V. In vitro excystation of Cryptosporidium parvum. Parasitology. 106:1993;13-29.
14. Vesey G., Slade J.S., Byrne M., Shepherd K., Fricker C.R. A new method for the concentration of Cryptosporidium oocysts from water. J. Appl. Bacteriol. 75:1993;82-86.