Improved assay sensitivity of an engineered secreted Renilla luciferase

Gene

Volumn 237, Issue 1, 1999, Pages 153-159

  Liu, Jingxue ^a   Escher, Alan ^a  

^a LOMA LINDA UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Bioluminescence; Disulfide bond; Eukaryotic gene expression; Reporter gene

Indexed keywords

LUCIFERASE;

AMINO ACID SEQUENCE; ANIMAL CELL; ARTICLE; BIOLUMINESCENCE; CELL CULTURE; CELL STRAIN COS1; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME RELEASE; GENE CONSTRUCT; GENE EXPRESSION; GENETIC TRANSFECTION; IN VITRO STUDY; MUTANT; NONHUMAN; PRIORITY JOURNAL;

ANIMALS; BIOLOGICAL ASSAY; CNIDARIA; COS CELLS; CULTURE MEDIA; ENZYME STABILITY; LUCIFERASES; LUMINESCENT MEASUREMENTS; MUTAGENESIS, SITE-DIRECTED; MUTATION; RECOMBINANT PROTEINS; SENSITIVITY AND SPECIFICITY; TRANSFECTION;

EID: 0032818467     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1119(99)00314-5     Document Type: Article

References (17)

1. Berger J., Hauber J., Hauber R., Geiger R., Cullen B.R. Secreted placental alkaline phosphatase: a powerful new quantitative indicator of gene expression in eukaryotic cells. Gene. 66:1988;1-10.
2. Cunningham B.C., Wells J.A. High-resolution epitope mapping of hGH-receptor interactions by alanine-scanning mutagenesis. Science. 244:1989;1081-1085.
3. Inouye S., Shimomura O. The use of Renilla luciferase, Oplophorus luciferase and apoaequorin as bioluminescent reporter protein in the presence of coelenterazine analogues as substrate. Biochem. Biophys. Res. Commun. 233:1997;349-353.
4. Jubin R., Murray M.G. Activity screening of bacteria containing Renilla luciferase plasmids. Biotechniques. 24:1998;185-188.
5. Liu J., O'Kane D.J., Escher A. Secretion of functional Renilla reniformis luciferase by mammalian cells. Gene. 203:1997;141-148.
6. Liu J., Filippova M., O'Kane D.J., Escher A. Secreted Renilla luciferase: a new marker of gene expression. Roda A., Pazzagli M., Kricka L.J., Stanley P.E. Bioluminescence and Chemiluminescence: Perspectives for the 21st Century. 1999;471-474 John Wiley, Chichester.
7. Lorenz W.W., McCann R.O., Longiaru M., Cormier M.J. Isolation and expression of a cDNA encoding Renilla reniformis luciferase. Proc. Natl. Acad. Sci. USA. 88:1991;4438-4442.
8. Lorenz W.W., Cormier M.J., O'Kane D.J., Hua D., Escher A., Szalay A.A. Expression of the Renilla reniformis luciferase gene in mammmalian cells. J. Biolumin. Chemilumin. 11:1996;31-37.
9. Matsumura M., Matthews B.W. Stabilization of functional proteins by introduction of multiple disulfide bonds. Methods Enzymol. 202:1991;336-357.
10. Matthews J.C., Hori K., Cormier M.J. Purification and properties of Renilla reniformis luciferase. Biochemistry. 16:1977;85-91.
11. Selden R.F., Howie K.B., Rowe M.E., Goodman H.M., Moore D.D. Human growth hormone as a reporter gene in regulation studies employing transient gene expression. Mol. Cell. Biol. 6:1986;3173-3179.
12. Sherf B.A., Navarro S.L., Hannah R.R., Wood K.V. Dual luciferase reporter assay: an advanced co-reporter technology integrating firefly and Renilla luciferase assays. Promega Notes. 57:1996;2-9.
13. Yang T.T., Sinai P., Kitts P.A., Kain S.R. Quantification of gene expression with a secreted alkaline phosphatase reporter system. Biotechniques. 23:1997;1110-1114.
14. Mayerhofer R., Langridge W.H.R., Cormier M.J., Szalay A.A. Expression of recombinant Renilla luciferase in transgenic plants results in high levels of light emission. Plant J. 7:1995;1031-1038.
15. Shimomura O., Masugi T., Johnson F.H., Haneda Y. Properties and reaction mechanism of the bioluminescence system of the deep-sea shrimp Oplophorus gracilorostris. Biochemistry. 17:1978;994-998.
16. Srikantha T., Klapach A., Lorenz W.W., Tsai L.K., Laughlin L.A., Gorman J.A., Soll D.R. The sea pansy Renilla reniformis luciferase serves as a sensitive bioluminescent reporter for differential gene expression in Candida albicans. J. Bacteriol. 178:1996;121-129.
17. Thompson E.M., Adenot P., Tsuji F.I., Renard J.P. Real time imaging of transcriptional activity in live mouse preimplantation embryos using a secreted luciferase. Proc. Natl. Acad. Sci. USA. 92:1995;1317-1321.