Micropurification and two-dimensional polyacrylamide gel electrophoresis of immunoglobulins for studying the clonal diversity of antigen-specific antibodies

Journal of Immunological Methods

Volumn 227, Issue 1-2, 1999, Pages 137-148

  Layer, Andréas ^a   Tissot, Jean Daniel ^b   Schneider, Philippe ^b   Duchosal, Michel A ^a  

^a LAUSANNE UNIVERSITY HOSPITAL   (Switzerland)

^b Service Regional Vaudois de Transfusion Sanguine   (Switzerland)

Author keywords

Affinity purification; Immunoglobulin; Monoclonal; Polyacrylamide gel electrophoresis; Polyclonal; Two dimensional

Indexed keywords

ANTIBODY; ANTIGEN; DITHIOERYTHRITOL; DODECYL SULFATE SODIUM; IMMUNOGLOBULIN G; RESIN; TETANUS TOXOID;

ANTIBODY SPECIFICITY; ARTICLE; CONTROLLED STUDY; HUMAN; HUMAN EXPERIMENT; HUMAN TISSUE; NORMAL HUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN PURIFICATION;

ANIMALS; ANTIBODY DIVERSITY; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; HUMANS; IMMUNOGLOBULIN G; IMMUNOGLOBULINS; MICE; MICE, SCID; TETANUS TOXOID;

EID: 0032817982     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-1759(99)00075-7     Document Type: Article

References (16)

1. Carlsson R., Martensson C., Kalliomäki S., Ohlin M., Borrebaeck C.A.K. Human peripheral blood lymphocytes transplanted into SCID mice constitute an in vivo culture system exhibiting several parameters found in a normal humoral immune response and are a source of immunocytes for the production of human monoclonal antibodies. J. Immunol. 148:1992;1065.
2. Celis J.E., Ostergaard M., Jensen N.A., Gromova I., Rasmussen H.H., Gromov P. Human and mouse proteomic databases: novel resources in the protein universe. FEBS Lett. 430(1-2):1998;64.
3. Duchosal M.A., Eming S.A., McConahey P.J., Dixon F.J. The hu-PBL-SCID mouse model. Long-term human serologic evolution associated with xenogeneic transfer of human peripheral blood leukocytes into SCID mice. Cell. Immunol. 139:1992;468.
4. Duchosal M.A., Eming S.A., McConahey P.J., Dixon F.J. Characterization of hu-PBL-SCID mice with high human immunoglobulin serum levels and graft-versus-host disease. Am. J. Pathol. 141:1992;1097.
5. Duchosal M.A., Eming S.A., Fischer P., Leturcq D., Barbas C.F., McConahey P.J., Caothien R.H., Thornton G.B., Dixon F.J., Burton D.R. Immunization of hu-PBL-SCID mice and rescue of human monoclonal Fab fragments through combinatorial libraries. Nature. 355:1992;258.
6. Gravel P., Sanchez J.C., Walzer C., Golaz O., Hochstrasser D.F., Balant L.P., Hughes G.J., Garcia-Sevilla J., Guimon J. Human blood platelet protein map established by two-dimensional polyacrylamide gel electrophoresis. Electrophoresis. 16:1995;115.
7. Hughes G.J., Frutiger S., Paquet N., Ravier F., Pasquali C., Sanchez J.C., James R., Tissot J.D., Bjellqvist B., Hochstrasser D.F. Plasma protein map: an update by microsequencing. Electrophoresis. 13:1992;707.
8. Hughes G.J., Frutiger S., Paquet N., Pasquali C., Sanchez J.C., Tissot J.D., Bairoch A., Appel R.D., Hochstrasser J.D. Human liver protein map: update 1993. Electrophoresis. 14:1993;1216.
9. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 227(259):1970;680.
10. Mosier D.E., Gulizia R.J., Baird S.M., Wilson D.B. Transfer of functional human immune system to mice with severe combined immunodeficiency. Nature. 335:1988;256.
11. O'Farrel P.H. High resolution two-dimensional electrophoresis of proteins. J. Biol. Chem. 250:1975;4007.
12. Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch A., Schaller D., Sanchez J.C., Hochstrasser D.F. Two-dimensional gel electrophoresis of Escherichia coli homogenates: the Escherichia coli SWISS-2DPAGE database. Electrophoresis. 17:1996;547.
13. Rabilloud T., Vuillard L., Gilly C., Lawrence J.J. Silver staining of proteins in polyacrylamide gels: a general overview. Cell. Mol. Biol. 40:1994;57.
14. Tissot J.D., Spertini F. Analysis of immunoglobulins by two-dimensional electrophoresis. J. Chromatogr. 698:1995;225.
15. Tissot J.D., Hochstrasser D.F., Spertini F., Schifferli J.A., Schneider P. Pattern variations of polyclonal and monoclonal immunoglobulins of different isotypes analyzed by high-resolution two-dimensional electrophoresis. Electrophoresis. 14:1993;227.
16. Tissot J.D., Schneider P., Schapira M., Duchosal M.A. Human immunoglobulins produced in hu-PBL-SCID mice are polyclonal early after xenotransplantation. Cell. Immunol. 167:1996;241.