Enzyme concentration as an important factor in the in vitro testing of the stereospecificity of the enzymatic hydrolysis of organophosphorus compounds

Toxicology in Vitro

Volumn 13, Issue 4-5, 1999, Pages 689-692

  Monroy Noyola, A ^b   Sogorb, M A ^a   Vilanova, E ^a  

^a MIGUEL HERNÁNDEZ UNIVERSITY   (Spain)

^b NONE

Author keywords

Organophosphorus compounds; Phosphotriesterases; Stereoisomers

Indexed keywords

ORGANOPHOSPHATE;

ANIMAL TISSUE; CHICKEN; CHIRAL CHROMATOGRAPHY; CONFERENCE PAPER; CONTROLLED STUDY; ENZYME ANALYSIS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HYDROLYSIS; IN VITRO STUDY; LIVER MICROSOME; NONHUMAN; RACEMIC MIXTURE; STEREOISOMERISM;

EID: 0032816929     PISSN: 08872333     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0887-2333(99)00066-1     Document Type: Conference Paper

References (8)

1. Díaz-Alejo N., Monroy A., Vilanova E., Vicedo J.L., Sogorb M.A. A stereospecific phosphotriesterase in hen liver and brain. Chemico-Biological Interactions. 108:1988;187-196.
2. Díaz-Alejo N., Vilanova E. Chiral high performance liquid chromatography and gas chromatography of the stereoisomers of hexyl 2,5-dichlorophenyl phosphoramidate. Journal of Chromatography. 622:1993;179-186.
3. Johnson M.K., Read D.J. The influence of chirality on the delayed neurophatic potential of some organophosphorus esters: neuropatic and protective effects of stereoisomeric esteres of ethylphenylphosphonic acid (EPN oxon and EPN) correlate with quantities of aged and unaged neuropathy target esterase in vivo. Toxicology and Applied Pharmacology. 90:1987;103-108.
4. Johnson M.K., Vilanova E., Read D.J. Biochemical and clinical test of delayed neuropathic potential of some O-alkyl O-dichlorphenyl phosphoramide analogues of metamidophos (O,S-dimethyl phosphorothioamidate). Toxicology. 54:1989;89-100.
5. Monroy-Noyola A., Sogorb M. A., Díaz-Alejo N., Ñíguez N., Barril J., Vicedo J. L. and Vilanova E. (1999) Dichlorophenyl phosphoramidates as substrates for avian and mammalian liver phosphotriesterases: activity levels, calcium dependence and stereospecificity. Chemico-Biological Interactions. 119 - 120, 257 - 262.
6. Sogorb M.A., Díaz-Alejo N., Escudero M.A., Vilanova E. Phosphotriesterase activity identified in purified serum albumins. Archives of Toxicology. 72:1998;219-226.
7. Sogorb M.A., Díaz-Alejo N., Pellín M.C., Vilanova E. Inhibition and aging of neuropathy target esterase by the stereoisomers of a phosphoramidate related to methamidophos. Toxicology Letters. 93:1997;95-102.
8. Vilanova E., Johnson M. K. and Vicedo J. L. (1987) Interaction of some unsubstituted phosphoramidate analogs of methamidophos (O,S-dimethyl phosphoro-thioamidate) with acetylcholinesterase and neuropathy target esterase of the hen brain. Pesticide Biochemistry and Physiology 28, 224-238.