Identifying mutations in drosophila genes by direct sequencing of PCR products

BioTechniques

Volumn 27, Issue 2, 1999, Pages 262-264

  Schlag, Erin M ^a   Wassarman, David A ^a  

^a EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH AND HUMAN DEVELOPMENT   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA; OLIGONUCLEOTIDE; PRIMER DNA;

ARTICLE; DNA SEQUENCE; DROSOPHILA; GENE MUTATION; GENETIC POLYMORPHISM; NONHUMAN; POLYMERASE CHAIN REACTION; TECHNIQUE;

EID: 0032815417     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/99272bm09     Document Type: Article

References (5)

1. Ashburner, M. 1989. Preparation of DNA from single flies, p. 108-109. In M. Ashburner (Ed.), Drosophila, A Laboratory Manual. CSH Laboratory Press, Cold Spring Harbor, NY.
2. Brunner, D., N. Oellers, J. Szabad, W.H. Biggs, S.L. Zipursky and E. Hafen. 1994. A gain-of-function mutation in Drosophila MAP kinase activates multiple receptor tyrosine kinase signaling pathways. Cell 76:875-888.
3. Chang, H.C., N.M. Solomon, D.A. Wassarman, F.D. Karim, M. Therrien, G.M. Rubin and T. Wolff. 1995. Phyllopod functions in the fate determination of a subset of photoreceptors in Drosophila. Cell 80:463-472.
4. Mori, A., A. Takagi, Y. Ikeda and A. Yamamoto. 1997. Development of a direct DNA sequencing method for detecting heterozygous mutations of the human lipoprotein lipase gene. Clin. Biochem. 30:315-324.
5. Therrien, M., H.C. Chang, N.M. Solomon, F.D. Karim, D.A. Wassarman and G.M. Rubin, 1995. KSR, a novel protein kinase required for Ras signal transduction. Cell 83:879-888.