Expression of a mammalian Na+/H+ antiporter in Saccharomyces cerevisiae

Biochemistry and Cell Biology

Volumn 77, Issue 1, 1999, Pages 25-31

  Montero Lomelí, Mónica ^a   Okorokova Façanha, Anna L ^a  

^a FEDERAL UNIVERSITY OF RIO DE JANEIRO   (Brazil)

Author keywords

Expression; Na+ H+ exchanger; NHE1; Yeast

Indexed keywords

5 (N ETHYL N ISOPROPYL)AMILORIDE; ANTIPORTER; GALACTOSE; HEAT SHOCK PROTEIN; PHOSPHATIDYLCHOLINE;

AMINO TERMINAL SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; ENDOPLASMIC RETICULUM; GENE MUTATION; NONHUMAN; PROTEIN EXPRESSION; PROTEIN INDUCTION; PROTEIN LOCALIZATION; SACCHAROMYCES CEREVISIAE;

AMINO ACID SEQUENCE; BLOTTING, WESTERN; CENTRIFUGATION, DENSITY GRADIENT; DOSE-RESPONSE RELATIONSHIP, DRUG; GENE EXPRESSION; MUTAGENESIS, SITE-DIRECTED; PLASMIDS; RECOMBINANT FUSION PROTEINS; RNA; SACCHAROMYCES CEREVISIAE; SODIUM-HYDROGEN ANTIPORTER; TEMPERATURE; TRANSFORMATION, GENETIC;

MAMMALIA; SACCHAROMYCES CEREVISIAE;

EID: 0032812193     PISSN: 08298211     EISSN: None     Source Type: Journal    
DOI: 10.1139/bcb-77-1-25     Document Type: Article

References (29)

1. 2+ ATPase homologue, PMR1, is required for normal Golgi funcion and localizes in a novel Golgi-like distribution. Mol. Biol. Cell, 3: 633-654.
2. + secretion in the proximal tubule..Am. J. Physiol 245: F647-F659.
3. +/H antiporter (NHE1) in baculovirus-infected cells. Biochemisty, 30: 9510-9515.
4. Franzusoff, A., Rothblatt, J., and Schekman, R. 1991. Analysis of polypeptide transit through yeast secretory pathway In Methods in enzymology. Edited by C.H. Guthrie and G.R. Fink. Academic Press, San Diego, Calif. pp. 662-674.
5. + exchanger: an update on structure, regulation and cardiac physiology. Biochem. J. 296: 273-285.
6. Garciadeblas, B., Rubio, F., Quintero, F.J., Bañuelos, M.A., Haro, R., and Rodriguez-Navarro, A. 1993. Differential expression of two genes encoding isoforms of the ATPase involved in sodium efflux in saccharomyces cerevisiae. Mol. Gen. Genet. 236: 363-368.
7. + exchanger. J. Membr. Biol. 90: 1-12.
8. Haggerty, J.G., Agarwal, N., Reilly, R.F., Adelberg, E.A., and Slayman, C.W. 1988. Pharmacologically different Na/H antiporters on the apical and basolateral surfaces of cultured porcine kidney cells (LLC-PK1). Proc. Natl. Acad. Sci. U.S.A. 85: 6797-6801.
9. + exchanger. Biochem. J. 289: 637-640.
10. Ito, H., Fukuda, Y., Murata, K., and Kimura, A. 1983. Transformation of intact yeast cells treated with alkali cations. J. Bacteriol. 153: 163-168.
11. + exchanger (NHE5/SLC9a5) to chromosome 16q22.1. Genomics, 25: 615-22.
12. Lowry, O.H., Rosenbrough, N.J., Farra, A.L., and Randall, R.J. 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193: 265-275.
13. +]-ATPase in secretory vesicles. J. Biol. Chem. 266: 7940-7949.
14. + exchanger. J. Biol. Chem. 273: 6951-6959.
15. Orlowski, J., Kandasamy, R.A., and Shull, G.E. 1992. Molecular cloning of putative members of the Na/H exchanger gene family. J. Biol. Chem. 267: 9331-9339.
16. +-ATPase a subunit: Site-directed mutagenesis of glutamine-111 to arginine and asparagine-122 to aspartic acid generates a ouabain-resistant enzyme. Biochemistry, 27: 8400-8408.
17. +-antiporter of the yeast Saccharomyces cerevisiae. FEBS Lett. 387: 89-93.
18. +/K exchange in reconstituted yeast plasma membrane vesicles. Biochem. Biophys. Acta 1285: 175-182.
19. + exchanger in LLC-PK1 renal epithelial cells. Am. J. Physiol. 261: F1088-F1094.
20. Ruetz, S., Raymond, M., and Gros, P. 1993. Functional expression of a P-glycoprotein encoded by the mouse mdr3 gene in yeast cells. Proc. Natl. Acad. Sci. U.S.A., 90: 11 588-11 592.
21. + antiporter. Cell, 56: 271-280.
22. 2+-ATPases. Nature (London), 319: 689-693.
23. Tse, C.M., Ma, A.I., Yang, V.W., Watson, A.J.M., Levine, S., Montrose, M.H., Potter, J., Sardet, C., Pouysségur, J., and Donowitz, M. 1991. Molecular cloning and expression of a cDNA encoding the rabbit ileal villus cell basolateral membrane Na/H exchanger. EMBO J. 10: 1957-1967.
24. Tse, C.M., Brant, S.R., Walker, S., Pouyssegur, J., and Donowitz, M. 1992. Cloning and sequencing of a rabbit cDNA encoding an intestinal and kidney-specific Na/H exchanger isoform (NHE3). J. Biol. Chem. 267: 9340-9346.
25. + exchanger gene family-initial structure/function studies. J. Am. Soc. Nephrol. 4: 969-975.
26. + exchanger isoform (NHE2). J. Biol. Chem. 268: 11 917-11 924.
27. + antiporter. Biochim. Biophys. Acta, 1144: 367-373.
28. + pump in the yeast plasma membrane. EMBO J. 14: 3870-3882.
29. + exchanger. Annu. Rev. Physiol. 55: 289-304.