Localization of lead in Allium cepa L. cells by electron microscopy

Journal of Microscopy

Volumn 195, Issue 2, 1999, Pages 139-146

  Antosiewicz, D ^a   Wierzbicka, M ^a  

^a UNIVERSITY OF WARSAW   (Poland)

Author keywords

Allium cepa; Fixing and dehydrating procedure; Lead; Localization of lead; Root tip; X ray microanalysis

Indexed keywords

ACETONE; CELLS; CHEMICAL INDUSTRY; CYTOLOGY; DEHYDRATION; ELECTRON MICROSCOPES; GALLIUM COMPOUNDS; HISTOLOGY; LEAD COMPOUNDS; TISSUE;

ALLIUM CEPA; CHEMICALS PROCESSING; CONVENTIONAL TECHNIQUES; FIXING AND DEHYDRATING PROCEDURE; L-CELLS; LEAD; LOCALISATION; LOCALIZATION OF LEAD; ROOT TIP; X-RAY MICROANALYSIS;

ELECTRONS;

LEAD; OSMIUM TETRAOXIDE;

ARTICLE; DEHYDRATION; ELECTRON MICROSCOPY; ONION; PLANT ROOT; PRIORITY JOURNAL; X RAY MICROANALYSIS;

ALLIUM CEPA;

EID: 0032811042     PISSN: 00222720     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2818.1999.00492.x     Document Type: Article

References (39)

1. Baker, A.J.M. & Walker, P.L. (1990) Ecophysiology of metal uptake by tolerant plants. Heavy Metal Tolerance in Plants: Evolutionary Aspects (ed. by J. Shaw). pp. 155-177. CRC Press, Inc., Boca Raton, Florida.
2. Davies, K.L., Davies, M.S. & Francis, D. (1991) Zinc-induced vacuolation in root meristematic cells of Festuca rubra L. Plant Cell Environ. 14, 399-406.
3. Ernst, W.H.O. (1990) Mine vegetation in Europe. Heavy Metal Tolerance in Plants: Evolutionary Aspect (ed. by J. Shaw). pp. 21-37. CRC Press, Inc., Boca Raton, Florida.
4. Ernst, W.H.O. (1998) Effects of heavy metals in plants at the cellular and organismic level. Ecotoxicology (ed. by G. Schü̈rmann and B. Markert), pp. 587-619. John Wiley & Sons, Inc., & Spektrum Akademischer-Verlag.
5. Fritz, E. (1980) Microautoradiographic localization of assimilates in phloem: problems and new method. Ber. Deutsch. Bot. Ges. 93, 109-121.
6. Harvey, D.M.R. (1980) The preparation of botanical samples for ion localization studies at the subcellular level. Scan. Electron Microsc. 2, 409.
7. Harvey, D.M.R., Hall, J.L. & Flowers, T.J. (1976) The use of freeze-substitution in the preparation of plant tissue for ion localization studies. J. Microsc. 107, 189-198.
8. Hodgson, M.J. & Sangster, A.G. (1989) Subcellular localizalion of mineral deposits in the roots of wheat (Triticum aestivum L.). Protoplasma, 151, 19-32.
9. Hübner, R., Depta, H. & Robinson, D.G. (1985) Endocytosis in maize root cap cells, evidence obtained using heavy metal salt solutions. Protoplasma, 129, 214-222.
10. 2 on poplar tissue culture and the ultrastructural localization of lead in culture cells. For. Ecol. Manage. 8, 95-105.
11. Lane, S.D. & Martin, E.S. (1982) An ultrastructural examination of lead localization in germinating seeds of Raphanus sativus. Z. Pflanzenphysiol. 107, 33-40.
12. Malone, C., Koeppe, D.E. & Miller, R.J. (1974) Localization of lead accumulated by corn plants. Plant Physiol. 53, 388-394.
13. Mengel, K. & Kirkby, E.A. (1980) Principles of Plant Nutrition, 3rd edn. International Potash Institute, Worblaufen-Bern/Switzerland.
14. Michalak, E. & Wierzbicka, M. (1998) Differences in lead tolerance between Allium cepa plants developing from seeds and bulbs. Plant Soil, 199, 251-260.
15. Morgan, A.J. (1985) Thin-specimen preparation. X-Ray Microanalysis in Electron Microscopy for Biologists, pp. 52-65. Oxford University Press, Oxford.
16. Mullins, M., Hardwick, K. & Thurman, D.A. (1985) Heavy metal location by analytical electron microscopy in conventionally fixed and freeze-substituted roots of metal tolerant and non tolerant ecotypes. Heavy Metals in the Environment Vol. 2 (ed. by T. D. Lekkas). pp. 43-46. C.E.P. Consultants Ltd, Edinburgh.
17. Qureshi, J.A., Hardwick, K. & Collin, H.A. (1986) Intracellular localization of lead in lead tolerant and sensitive clones of Anthoxanthum odoratum. J. Plant Physiol. 122, 357-364.
18. Richter, G.W., Kress, M.D.Y. & Cornwall, C.C. (1968) Another look at lead inclusion bodies. Am. J. Pathol. 53, 189-218.
19. Rusiccki, W. & Kubikowski, P. ( 1969) Contemporary Toxicology. PZWL, Warsaw. (in Polish).
20. Sharpe, V. & Denny, P. (1976) Electron microscope studies on the absorption and localization of lead in the tissue of Potamogeton pectinatus L. J. Exp. Bot. 27, 1155.
21. Tomaszewska, B., Tukendorf, A. & Baralkiewicz, D. (1996) The synthesis of phytochelatins in lupin roots treated with lead ions. Sci. Legum. 3, 206-217.
22. Vandeputte, D.E., Jacob, W.A. & Van Gricken, R.E. (1990) Influence of fixation procedures on the microanalysis of lead-induced intranuclear inclusions in rat kidney. J. Histochem. Cytohem. 38, 331.
23. Vian, B. (1981) La technique des coupes en froid appliquée en matériel végétal. Bull. Soc. Bot. Fr. 128, 89-112.
24. Wierzbicka, M. (1987a) Lead accumulation and its translocation barriers in roots of Allium cepa L. - autoradiographic and ultrastructural studies. Plant Cell Environ. 10, 17-26.
25. Wierzbicka, M. (1987b) Lead translocation and localization in Allium cepa L. root. Can. J. Bot. 65, 1857-1860.
26. Wierzbicka, M. (1987c) An improved method of preparing onion bulbs for the Allium test. Acta Soc. Bot. Pol. 56, 43-53.
27. Wierzbicka, M (1988) Mitotic disturbances induced by low doses of inorganic lead. Caryologia, 41, 143-160.
28. Wierzbicka, M. (1989) Disturbances in cytokinesis caused by inorganic lead. Environ. Exp. Bot. 29, 123-133.
29. Wierzbicka, M. (1994) The resumption of mitotic activity in Allium cepa L. root tips during treatment with lead salts. Environ. Exp. Bot. 34, 173-180.
30. Wierzbicka, M. (1995) How lead loses its toxitity to plants. Acta Soc. Bot. Pol. 64, 81-90.
31. Wierzbicka, M. (1998) Lead in the apoplast of Allium cepa L. root tips - ultrastructural studies. Plant Sci. 133, 105-119.
32. Wierzbicka, M. (1999a) Comparison of lead tolerance in Allium cepa with other plant species. Environ. Pollut. 104, 41-52.
33. Wierzbicka, M. (1999b) The effect of lead on the cell cycle in the root meristem of Allium cepa L. Protoplasma, in press.
34. Wierzbicka, M. & Antosiewicz, D. (1988) Allium test - some questions. Acta Soc. Bot. Pol. 57, 201-215.
35. Wierzbicka, M. & Antosiewicz, D. (1995) How lead can easily enter the food chain - a study of plant roots. Sci. Total Environ. (Suppl. 1). 575-589.
36. Wierzbicka, M. & Obidzińska, J. (1998) The effect of lead on seed imbibition and germination in different plant species. Plant Sci. 137, 155-171.
37. Wierzbicka, M. & Panufnik, D. (1998) The adaptation of Silene. vulgaris to growth on a calamine waste heap (S. Poland). Environ. Pollut. 101, 415-426.
38. Woźny, A. & Krzeslowska, M. (1993) Plant cell responses to lead. Acta Soc. Bot. Pol. 62, 101-105.
39. Zenk, M.H. (1996) Heavy metal detoxification in higher plants - a review. Gene, 179, 21-30.