Interaction of Bacillus subtilis fur (ferric uptake repressor) with the dhb operator in vitro and in vivo

Journal of Bacteriology

Volumn 181, Issue 14, 1999, Pages 4299-4307

  Bsat, Nada ^a   Helmann, John D ^a  

^a Department of Obstetrics Gynecology   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ALANINE; BACTERIAL DNA; EDETIC ACID; FERRIC ION; FERROUS ION; HISTIDINE; IRON; METALLOPROTEIN; REGULATOR PROTEIN; RNA POLYMERASE; SIDEROPHORE;

ARTICLE; BACILLUS SUBTILIS; BACTERIAL GENETICS; CONTROLLED STUDY; DIALYSIS; GENE EXPRESSION; GENETIC TRANSCRIPTION; IN VITRO STUDY; IN VIVO STUDY; MUTAGENESIS; NONHUMAN; OPERATOR GENE; OPERON; PRIORITY JOURNAL; REPRESSOR GENE;

BACILLUS SUBTILIS; BACTERIA (MICROORGANISMS); POSIBACTERIA; PROKARYOTA;

EID: 0032811011     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.181.14.4299-4307.1999     Document Type: Article

References (42)

1. Althaus, E. W., C. E. Outten, K. E. Ohlsen, H. Cao, and T. V. O'Halloran. 1999. The ferric uptake regulation (Fur) repressor is a zinc metalloprotein. Biochemistry 38:6559-6569.
2. Arnow, L. E. 1937. Colorimetric determination of the components of 3,4 dihydroxyphenylalanine-tyrosine mixtures. J. Biol. Chem. 228:531-537.
3. Bagg, A., and J. B. Neilands. 1987. Ferric uptake regulation protein acts as a repressor, employing iron (II) as a cofactor to bind the operator of an iron transport operon in Escherichia coli. Biochemistry 26:5471-5477.
4. Bagg, N., and J. B. Neilands. 1987. Molecular mechanism of regulation of siderophore-mediated iron assimilation. Microbiol. Rev. 51:509-518.
5. Bearden, S. W., T. M. Staggs, and R. D. Perry. 1998. An ABC transporter system of Yersinia pestis allows utilization of chelated iron by Escherichia coli SAB11. J. Bacteriol. 180:1135-1147.
6. Braun, V. 1997. Avoidance of iron toxicity through regulation of bacterial iron transport. Biol. Chem. 378:779-786.
7. Bsat, N., L. Chen, and J. D. Helmann. 1996. Mutation of the Bacillus subtilis alkyl hydroperoxide reductase (ahpCF) operon reveals compensatory interactions among hydrogen peroxide stress genes. J. Bacteriol. 178:6579-6586.
8. Bsat, N., A. Herbig, L. Casillas-Martinez, P. Setlow, and J. D. Helmann. 1998. Bacillus subtilis contains multiple Fur homologs: identification of the iron uptake (Fur) and peroxide regulon (PerR) repressors. Mol. Microbiol. 29:189-198.
9. Chen, L., L. P. James, and J. D. Helmann. 1993. Metalloregulation in Bacillus subtilis: isolation and characterization of two genes differentially repressed by metal ions. J. Bacteriol. 175:5428-5437.
10. Chen, L., L. Keramati, and J. D. Helmann. 1995. Coordinate regulation of Bacillus subtilis peroxide stress genes by hydrogen peroxide and metal ions. Proc. Natl. Acad. Sci. USA 92:8190-8194.
11. Coy, M., C. Doyle, J. Besser, and J. B. Neilands. 1994. Site-directed mutagenesis of the ferric uptake regulation gene of Escherichia coli. BioMetals 7:292-298.
12. Coy, M., and J. B. Neilands. 1991. Structural dynamics and functional domains of the Fur protein. Biochemistry 30:8201-8210.
13. Cutting, S. M., and P. B. VanderHorn. 1990. Genetic analysis, p. 27-74. In C. R. Harwood and S. M. Cutting (ed.), Molecular biological methods for Bacillus. John Wiley & Sons, Ltd., Chichester, England.
14. de Lorenzo, V., S. Wee, M. Herrero, and J. B. Neilands. 1987. Operator sequences of the aerobactin operon of plasmid CoIV-K30 binding the ferric uptake regulation (fur) repressor. J. Bacteriol. 169:2624-2630.
15. Escolar, L., V. de Lorenzo, and J. Perez-Martín. 1997. Metalloregulation in vitro of the aerobactin promoter of Escherichia coli by the Fur (ferric uptake regulation) protein. Mol. Microbiol. 26:799-808.
16. Escolar, L., J. Perez-Martin, and V. de Lorenzo. 1998. Coordinated repression in vitro of the divergent fepA-fes promoters of Escherichia coli by the iron uptake regulation (Fur) protein. J. Bacteriol. 180:2579-2582.
17. Gaballa, A., and J. D. Helmann. 1998. Identification of a zinc-specific metalloregulatory protein, Zur, controlling zinc transport operons in Bacillus subtilis. J. Bacteriol. 180:5815-5821.
18. Guerinot, M. L. 1994. Microbial iron transport. Annu. Rev. Microbiol. 48: 743-772.
19. Guérout-Fleury, A.-M., N. Frandsen, and P. Stragier. 1996. Plasmids for ectopic integration in Bacillus subtilis. Gene 180:57-61.
20. Hall, H. K., and J. W. Foster. 1996. The role of Fur in the acid tolerance response of Salmonella typhimurium is physiologically and genetically separable from its role in iron acquisition. J. Bacteriol. 178:5683-5691.
21. Hantke, K. 1987. Selection procedure for deregulated iron transport mutants (fur) in Escherichia coli K.-12: fur not only affects iron metabolism. Mol. Gen. Genet. 210:135-139.
22. Hantke, K., and V. Braun. 1997. Control of bacterial iron transport by regulatory proteins, p. 11-44. In S. Silver and W. Walden (ed.), Metal ions in gene regulation. Chapman & Hall, New York, N.Y.
23. Helmann, J. D. 1997. Metal cation regulation in Gram-positive bacteria, p. 45-76. In S. Silver and W. Walden (ed.), Metal ions in gene regulation. Chapman & Hall, New York, N.Y.
24. Jacquamet, L., D. Aberdam, A. Adrait, J.-L. Hazemann, J.-M. Latour, and I. Michaud-Soret. 1998. X-ray absorption spectroscopy of a new zinc site in the Fur protein from Escherichia coli. Biochemistry 37:2564-2571.
25. Juang, Y. L., and J. D. Helmann. 1994. The δ subunit of Bacillus subtilis RNA polymerase: an allosteric effector of the initiation and core-recycling phases of transcription. J. Mol. Biol. 239:1-14.
26. Juang, Y. L., and J. D. Helmann. 1994. A promoter melting region in the primary σ factor of Bacillus subtilis: identification of functionally important aromatic amino acids. J. Mol. Biol. 235:1470-1488.
27. Keyer, K., and J. A. Imlay. 1996. Superoxide accelerates DNA damage by elevating free-iron levels. Proc. Natl. Acad. Sci. USA 93:13635-13640.
28. Neilands, J. B. 1993. Siderophores. Arch. Biochem. Biophys. 302:1-3.
29. Neilands, J. B. 1995. Siderophores: structure and function of microbial iron transport compounds. J. Biol. Chem. 270:26723-26726.
30. Ochsner, U. A., A. I. Vasil, and M. L. Vasil. 1995. Role of the ferric uptake regulator of Pseudomonas aeruginosa in the regulation of siderophores and exotoxin A expression: purification and activity on iron-regulated promoters. J. Bacteriol. 177:7194-7201.
31. Perego, M. 1993. Integrational vectors for genetic manipulation in Bacillus subtilis, p. 615-624. In A. L. Sonenshein (ed.), Bacillus subtilis and other gram-positive bacteria: biochemistry, physiology, and molecular genetics. American Society for Microbiology, Washington, D.C.
32. Privalle, C. T., and I. Fridovich. 1993. Iron-specificity of the Fur-dependent regulation of the biosynthesis of the manganese-containing superoxide dismutase in Escherichia coli. J. Biol. Chem. 268:5178-5181.
33. Rowland, B. M., and H. Taber. 1996. Duplicate isochorismate synthase genes of Bacillus subtilis: regulation and involvement in the biosyntheses of menaquinone and 2,3-dihydroxybenzoate. J. Bacteriol. 178:854-861.
34. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
35. Séraphin, B., and S. Kandels-Lewis. 1996. An efficient PCR mutagenesis strategy without gel purification that is amenable to automation. Nucleic Acids Res. 24:3276-3277.
36. Slack, F. J., J. P. Mueller, and A. L. Sonenshein. 1993. Mutations that relieve nutritional repression of the Bacillus subtilis dipeptide permease operon. J. Bacteriol. 175:4605-4614.
37. Stojilkovic, I., and K. Hantke. 1995. Functional domains of the Escherichia coli ferric uptake regulator protein (Fur). Mol. Gen. Genet. 247:199-205.
38. Studier, F. W. 1991. Use of bacteriophage lysozyme to improve an inducible T7 expression System. J. Mol. Biol. 219:37-44.
39. Studier, F. W., A. H. Rosenberg, J. J. Dunn, and J. W. Dubendorff. 1990. Use of T7 RNA polymerase to direct expression of cloned genes. Methods Enzymol. 185:60-89.
40. Wee, S., J. B. Neilands, M. L. Bittner, B. C. Hemming, B. L. Haymore, and R. Seetharam. 1988. Expression, isolation and properties of Fur (ferric uptake regulation) protein of Escherichia coli K12. Biol. Metals 1:62-68.
41. Youngman, P. 1990. Use of transposons and integrational vectors for mutagenesis and construction of gene fusions in Bacillus species, p. 221-266. In C. R. Harwood and S. M. Cutting (ed.), Molecular biological methods for Bacillus. John Wiley & Sons, Ltd., Chichester, England.
42. Zuber, P., and R. Losick. 1987. Role of AbrB in Spo0A- and Spo0B-dependent utilization of a sporulation promoter in Bacillus subtilis. J. Bacteriol. 169:2223-2230.