Engineering endoglucanase-secreting strains of ethanologenic Klebsiella oxytoca P2

Journal of Industrial Microbiology and Biotechnology

Volumn 22, Issue 6, 1999, Pages 600-607

  Zhou, S ^a   Ingram, L O ^a  

^a UNIVERSITY OF FLORIDA   (United States)

Author keywords

Biomass; Endoglucanase; Erwinia; Ethanol; Klebsiella; Lignocellulose

Indexed keywords

ALCOHOL; CELLOBIOSE; GLUCAN SYNTHASE;

ARTICLE; BACTERIAL GENE; BACTERIAL METABOLISM; ENZYME RELEASE; ERWINIA; GENETIC RECOMBINATION; KLEBSIELLA OXYTOCA; NONHUMAN; PROMOTER REGION; ZYMOMONAS MOBILIS;

BACTERIA (MICROORGANISMS); ERWINIA; ERWINIA CHRYSANTHEMI; KLEBSIELLA; KLEBSIELLA OXYTOCA; NEGIBACTERIA; ZYMOMONAS MOBILIS;

EID: 0032809418     PISSN: 13675435     EISSN: None     Source Type: Journal    
DOI: 10.1038/sj.jim.2900666     Document Type: Article

References (38)

1. 1 Ausubel FM, R Brent, RE Kingston, DD Moore, JG Seigman, JA Smith and K Struhl. 1998. Current Protocols in Molecular Biology. John Wiley & Sons, New York, NY.
2. 2 Barras F, F Gijsegem and AK Chatterjee. 1994. Extracellular enzymes and pathogenesis of soft-rot Erwinia. Annu Rev Phytopathol 32: 201-234.
3. 3 Beall DS and LO Ingram. 1993. Genetic engineering of soft-rot bacteria for ethanol production from lignocellulose. J Ind Microbiol 11: 151-155.
4. 4 Christakopoulos P, BJ Macris and D Kekos. 1990. On the mechanism of direct conversion of cellulose to ethanol by Fusarium oxysporum: effect of cellulase and β-glucosidase. Appl Microbiol Biotechnol 33: 18-20.
5. 2 fixation in Klebsiella pneumoniae. Biotechnol Lett 20: 489-493.
6. 6 Cutting SM and PBV Horn. 1990. Genetic analysis. In: Molecular Methods for Bacillus (Harwood CR and SM Cutting, eds), pp 61-74. John Wiley & Sons, New York, NY.
7. 7 Dombek KM and LO Ingram. 1986. Magnesium limitation and its role in the apparent toxicity of ethanol during yeast fermentation. Appl Environ Microbiol 52: 975-981.
8. 8 Doran JB, HC Aldrich and LO Ingram. 1994. Saccharification and fermentation of sugar cane bagasse by Klebsiella oxytoca P2 containing chromosomally integrated genes encoding the Zymomonas mobilis ethanol pathway. Biotechnol Bioeng 44: 240-247.
9. 9 Gauss WF, S Suzuki and M Takagi. 1976. Manufacture of alcohol from cellulosic material using plural ferments. US Patent No. 3 990 944.
10. 10 Guiseppi A, JL Aymeric, B Cami, F Barras and N Creuzet. 1991. Sequence analysis of the cellulase-encoding celY gene of Erwinia chrysanthemi: a possible case of interspecies gene transfer. Gene 106: 109-114.
11. 11 Hamilton CM, M Aldea, BK Washburn, P Babitzke and SR Kushner. 1989. New methods for generating deletions and gene replacements in Escherichia coli. J Bacteriol 171: 4617-4622.
12. 12 He SY, M Lindeberg, AK Chatterjee and A Collmer. 1991. Cloned Erwinia chrysanthemi out genes enables Escherichia coli to selectively secret a diverse family of heterologous proteins to its milieu. Proc Natl Acad Sci USA 88: 1079-1083.
13. 13 Himmel ME, WS Adney, JO Baker, R Elander, JD McMillan, RA Nieves, JJ Sheehan, SR Thomas, TB Vinzant and M Zhang. 1997. Advanced bioethanol production technologies: a perspective. In: Fuels and Chemicals from Biomass (Saha BC and J Woodward, eds), pp 2-45, American Chemical Society, Washington, DC.
14. 14 Hogsett DA, HJ Ahn, TD Bernardez, CR South and LR Lynd. 1992. Direct microbial conversion: prospects, progress, and obstacles. Appl Biochem Biotechnol 34-35: 527-542.
15. 15 Ingram LO and JB Doran. 1995. Conversion of cellulosic materials to ethanol. FEMS Microbiol Rev 16: 235-241.
16. 16 Ingram LO, PF Gomez, X Lai, M Moniruzzaman, BE Wood, LP Yomano and SW York. 1998. Metabolic engineering of bacteria for ethanol production. Biotechnol Bioeng 58: 204-214.
17. 17 Ingram LO and T Conway. 1988. Expression of different levels of ethanologenic enzymes from Zymomonas mobilis in recombinant strains of Escherichia coli. Appl Environ Microbiol 54: 397-404.
18. 18 Ingram LO, T Conway, DP Clark, GW Sewell and JF Preston. 1987. Genetic engineering of ethanol production in Escherichia coli. Appl Environ Microbiol 53: 2420-2425.
19. 19 Minamigochi K, T Ooi, T Kawagochi, K Okada, S Murao and M Arai. 1995. Secretive expression of the Aspergillus aculeatus cellulase (FI-CMCase) by Saccharomyces cerevisiae. J Ferment Bioeng 79: 363-366.
20. 20 Murata H, M Fons, A Chatterjee, A Collmer and AK Chatterjee. 1990. Characterization of transposon insertion out" mutants of Erwinia carotovora subsp carotovora defective in enzyme export and a DNA segment that complements out mutants in E. carotovora subsp carotovora, E. carotovora subsp atroseptica, and Erwinia chrysanthemi. J Bacteriol 172: 2970-2978.
21. 21 Ohta K, DS Beall, JP Mejia, KT Shanmugam and LO Ingram. 1991. Genetic improvement of Escherichia coli for ethanol production: chromosomal integration of Zymomonas mobilis genes encoding pyruvate decarboxylase and alcohol dehydrogenase II. Appl Environ Microbiol 57: 893-900.
22. 22 Okamoto T, S Yamano, H Ikeaga and K Nakamura. 1994. Cloning of the Acetobacter xylinum cellulase gene and its expression in Escherichia coli and Zymomonas. Appl Microbiol Biotechnol 42: 563-568.
23. 23 Philippidis GP. 1994. Cellulase production technology: evaluation of current status. In: Enzymatic Conversion of Biomass for Fuel Production (Himmel ME, JO Baker and RP Overend, eds), pp 188-217, American Chemical Society, Washington, DC.
24. 24 Posfai G, MD Koob, HA Kirkpatrick and FR Blattner. 1997. Versatile insertion plasmids for targeted genome manipulations in bacteria: isolation, deletion, and rescue of the pathogenicity island LEE of the Escherichia coli 0157:H7 genome. J Bacteriol 179: 4426-4428.
25. 25 Pugsley AP. 1993. The complete general secretory pathway in Gram-negative bacteria. Microbiol Rev 57: 50-108.
26. 26 Py B, GPC Salmond, M Chippaux and F Barras. 1991. Secretion of cellulase in Erwinia chrysanthemi and E. carotovora is species specific. FEMS Microbiol Lett 79: 315-322.
27. 27 Py B, I Bortoli-German, J Haiech, M Chuppaux and F Barras. 1991. Cellulase EGZ of Erwinia chrysanthemi: structural organization and importance of His98 and Glu133 residues of catalysis. Protein Engin 4: 325-333.
28. 28 Sambrook J, EF Fritsch and T Maniatis. 1989. Molecular Cloning: A Laboratory Manual, 2nd edn. pp 1-478, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
29. 29 Saito T, T Suzuki, A Hayashi, H Honda, M Taya, S Iijima and T Kobayashi. 1990. Expression of a thermostable cellulase gene from a thermophilic anaerobe in Saccharomyces cerevisiae. J Ferment Bioeng 69: 282-286.
30. 30 Sauvonnet N and AP Pugsley. 1996. Identification of two regions of Klebsiella oxytoca pullulanase that together are capable of promoting β-lactamase secretion by the general secretory pathway. Mol Microbiol 22: 1-7.
31. 31 Sheehan JJ. 1994. Bioconversion for production of renewable transportation fuels in the United States: a strategic perspective. In: Enzymatic Conversion of Biomass for Fuel Production (Himmel ME, JO Baker and RP Overend, eds), pp 1-52, American Chemical Society, Washington, DC.
32. 32 Su P, CQ Liu, RJ Lucas, SF Delaney and NW Dunn. 1993. Simultaneous expression of genes encoding endoglucanase and β-glucosidase in Zymomonas mobilis. Biotechnol Lett 15: 979-984.
33. 33 Vroemen S, J Heldens, C Boyd, B Henrissat and NT Keen. 1995. Cloning and characterization of the bgxΛ gene from Erwinia chrysanthemi D1 which encodes a β-glucosidase/xylosidase enzyme. Mol Gen Genet 246: 465-477.
34. 34 Wood BE, DS Beall and LO Ingram. 1997. Production of recombinant bacterial endoglucanase as a co-product with ethanol during fermentation using derivatives of Escherichia coli KO11. Biotech Bioeng 55: 547-555.
35. 35 Wood BE and LO Ingram. 1992. Ethanol production from cellobiose, amorphous cellulose and crystalline cellulose by recombinant Klebsiella oxytoca containing chromosomally integrated Zymomonas mobilis genes for ethanol production and plasmids expressing thermostable cellulase genes from Clostridium thermocellum. Appl Environ Microbiol 58: 2103-2110.
36. 36 Wood TM and KM Bhat. 1988. Methods for measuring cellulase activities. In: Methods in Enzymology, vol 160 (Wood WA and ST Kellogg, eds), pp 87-112, Academic Press, San Diego, CA.
37. 37 Wyman CE. 1995. Economic fundamentals of ethanol production from lignocellulosic biomass. In: Enzymatic Degradation of Insoluble Carbohydrates (Saddler JN and MH Penner, eds), pp 272-290, American Chemical Society, Washington, DC.
38. 38 Yomano LP, RK Scopes and LO Ingram. 1993. Cloning, sequencing and expression of the Zymomonas mobilis phosphoglycerate mutase gene (pgm) in Escherichia coli. J Bacteriol 175: 3926-3933.