Analysis of viral glycoproteins by glycosidic digestion inside a polyacrylamide gel

Journal of Virological Methods

Volumn 80, Issue 2, 1999, Pages 217-221

  Grutadauria, Sergio ^a   Castilla, Viviana ^b   Zapata, Marta ^a   Mersich, Susana ^b  

^a FACULTAD DE CIENCIAS MÉDICAS   (Argentina)

^b Fac Cie E   (Argentina)

Author keywords

Glycosidic residues; Junin virus; Rubella virus; Viral glycoproteins

Indexed keywords

GLYCOSIDASE; POLYACRYLAMIDE GEL; VIRUS GLYCOPROTEIN;

HYDROLYSIS; JUNIN VIRUS; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN ANALYSIS; RUBELLA VIRUS; SHORT SURVEY;

JUNIN VIRUS; RUBELLA VIRUS;

EID: 0032808242     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0166-0934(99)00005-1     Document Type: Article

References (16)

1. Arthur L.O., Bess J.W., Sowder R.C. II, Benveniste R.E., Mann D.L., Chermann J.C., Henderson L.E. Cellular proteins bound to immunodeficiency viruses: implications for pathogenesis and vaccines. Science. 258:1992;1935-1938.
2. Baldick C.J., Shenk T. Proteins associated with purified human cytomegalovirus particles. J. Virol. 70:1996;6097-6105.
3. Beeley J.G. Structural Analysis. Glycoproteins and Proteoglycan Techniques. 1985;Elsevier, Amsterdam.
4. Castilla, V., 1995. Junin virus. Mechanism of entry to the host cell. Characterization and biological function of the major glycoprotein. Ph.D. Thesis. University of Buenos Aires, Argentina.
5. Cleveland D. W., Fischer S.G., Kirschner M.W., Laemmli U.K. Peptide mapping by limited proteolysis in sodium dodecyl sulphate and analysis by gel electrophoresis. J. Biol. Chem. 252:1977;1102-1106.
6. Contigiani M.S, Sabattini M.S. Virulencia diferencial de cepas de virus Junín por marcadores biológicos en ratones y cobayos. Medicina (Buenos Aires). 37:1977;244-251.
7. Cordoba P., Grutadauria S., Cuffini C., Zapata M. Presence of a neutralizing domain in several strains and isolates of rubella virus. Clin. Diagn. Lab. Immunol. 4:1997;493-495.
8. Chantler J.K., Lund K.D., Miki N.P.H., Berkowitz C.A., Tai G. Characterization of RV strain differences associated with attenuation. Intervirology. 36:1993;225-236.
9. Gretch D.R., Gehrz R.C., Stinski M.F. Characterization of a human cytomegalovirus glycoprotein complex (gCI). J. Gen. Virol. 69:1988;1205-1215.
10. Grutadauria, S., 1998. Comparative analysis among strains of rubella virus: biological and structural characteristics. Ph.D. Thesis. Universidad Nacional de Cordoba, Argentina.
11. Hobman C.H., Qiu Z., Chaye H., Gillam S. Analysis of rubella virus E1 glycosylation mutants. Virology. 181:1991;768-772.
12. Kühn J.E., Eing B.R., Brossmer R., Munk K., Brown R.W. Removal of N-linked carbohydrates decreases the infectivity of herpes simplex virus type 1. J. Gen. Virol. 69:1988;2847-2858.
13. Lundström M.L., Mauracher C.A., Tingle A.J. Characterization of carbohydrates linked to rubella virus glycoprotein E2. J. Gen. Virol. 72:1991;843-850.
14. Mersich S.E., Castilla V., Damonte E.B. Lectin affinity of Junin virus glycoproteins. Ann. Inst. Pasteur/Virol. 139:1988;277-284.
15. Pinter A., Honnen W.J. O-linked glycosylation of retroviral envelope gene products. J. Virol. 62:1988;1016-1021.
16. Sanchez A., Frey T.K. Vaccinia-vectored expression of the rubella virus structural proteins and characterization of the E1 and E2 glycosidic linkages. Virology. 183:1991;636-646.