A submersion method to induce hypoxic damage in organotypic hippocampal cultures

Journal of Neuroscience Methods

Volumn 89, Issue 1, 1999, Pages 25-31

  Frantseva, Marina V ^a   Carlen, Peter L ^a,b   El Beheiry, Hossam ^a,b  

^a UNIVERSITY OF TORONTO   (Canada)

^b TORONTO WESTERN HOSPITAL   (Canada)

Author keywords

Glucose deprivation; Hypothermia; Hypoxia; Neuronal cell death; Organotypic hippocampal cultures; Phenytoin; Propidium iodide

Indexed keywords

PHENYTOIN; PROPIDIUM IODIDE;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; BIOLOGICAL MODEL; BRAIN INJURY; BRAIN SLICE; CELL DEATH; CONTROLLED STUDY; GLYCOLYSIS; HIPPOCAMPUS; HYPOTHERMIA; HYPOXIA; ISCHEMIA; NEWBORN; NONHUMAN; PRIORITY JOURNAL; RAT; TECHNIQUE;

ANIMALS; BRAIN ISCHEMIA; CELL DEATH; CELL HYPOXIA; CULTURE MEDIA; ELECTRIC STIMULATION; HIPPOCAMPUS; MODELS, NEUROLOGICAL; NEURONS; ORGAN CULTURE TECHNIQUES; PATCH-CLAMP TECHNIQUES; PHENYTOIN; PYRAMIDAL CELLS; RATS; RATS, WISTAR;

EID: 0032806939     PISSN: 01650270     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0165-0270(99)00030-8     Document Type: Article

References (23)

1. Abdel-Hamid K.M., Tymianski M. Mechanisms and effects of intracellular calcium buffering on neuronal survival in organotypic hippocampal cultures exposed to anoxia/aglycemia or to excitotoxins. J. Neurosci. 17(10):1997;3538-3553.
2. Auer R.N., Siesjo B.K. Biological differences between ischemia, hypoglycemia, and epilepsy. Ann. Neurol. 24:1988;699-707.
3. Auer R.N., Wieloch T., Olsson Y., Siesjo B.K. The distribution of hypoglycemic brain damage. Acta. Neuropathol. (Berl). 67:1984;25-36.
4. Barone F.C., Feuerstein G.Z., White R.F. Brain cooling during transient focal ischemia provides complete neuroprotection. Neurosci. Biobehav. Rev. 21(1):1997;31-44.
5. Boehm F.H., Liem L.K., Stanton P.K., Potter P.E., Moskal J.R. Phenytoin protects against hypoxia-induced death of cultured hippocampal neurons. Neurosci. Lett. 175(1-2):1994;171-174.
6. Boxer PA, Cordon JJ, Mann ME, Rodolsi LC, Vartanian MG, Rock DM, Taylor CP, Marcoux FW. Comparison of phenytoin with noncompetitive NMDA antagonists in a model of focal brain ischemia in rat. Stroke 1990;21(11 Suppl):11147-51.
7. Bruno V.M., Goldberg M.P., Dugan L.L., Giffard R.G., Choi D.W. Neuroprotective effect of hypothermia in cortical cultures exposed to oxygen-glucose deprivation or excitatory amino acids. J. Neurochem. 63(4):1994;1398-1406.
8. Frantseva M.V., Jahromi S.S., Carlen P.L., El-Beheiry H. A static perfusion model of ischemia in organotypic hippocampal cultures in vitro. Soc. Neurosci. Abst. 24(1):1998;982.
9. Fried E., Amorin P., Chambers G., Cottrell J.E., Kass I.S. The importance of sodium for anoxic transmission damage in rat hippocampal slices: mechanisms of protection by lidocaine. J. Physiol. 489(2):1995;557-567.
10. Hayakawa T., Hamada Y., Maihara T., Hattori H., Mikawa H. Phenytoin reduces neonatal hypoxic-ischemic brain damage in rats. Life Sci. 54(6):1994;387-392.
11. Monyer H., Goldberg M.P., Choi D.W. Glucose deprivation neuronal injury in cortical culture. Brain Res. 483:1989;347-354.
12. Newman G.C., Qi H., Hospod F.E., Grundhmann K. Preservation of hippocampal brain slices with in vivo or in vitro hypothermia. Brain Res. 575(1):1992;159-163.
13. Newell D.W., Malouf A.T., Franck J.E. Glutamate mediated selective vulnerability to ischemia is present in organotypic cultures of hippocampus. Neurosci. Lett. 116:1990;325-330.
14. Sirimanne ES, Blumberg RM, Bossano D, Gunning M, Edwards AD, Gluckman PD, Williams CE. The effect of prolonged modification of cerebral temperature on outcome after hypoxic-ischemic brain injury in the infant rat. Ped Res 1996;39(4 Pt 1):591-7.
15. Stoppini L., Buchs L.-A., Muller D. A simple method for organotypic cultures of nervous tissue. J. Neurosci. Meth. 37:1991;173-182.
16. Strasser U., Fisher G. Protection from neuronal damage induced by combined oxygen and glucose deprivation in organotypic hippocampal cultures by glutamate receptor antagonists. Brain Res. 687:1995;167-174.
17. Taft W.C., Clifton G.L., Blair R.E., DeLorenzo R.J. Phenytoin protects against ischemia-produced neuronal cell death. Brain Res. 483:1989;143-148.
18. Tasker R.C., Coyle J.T., Vornov J.J. The regional vulnerability to hypoglycemia-induced neurotoxicity in organotypic hippocampal culture: protection by early tetrodotoxin or delayed MK-801. J. Neurosci. 12:1992;4298-4308.
19. Thoresen M., Wyatt J. Keeping a cool head, post-hypoxic hypothermia-an old idea revisited. Comment in: Acta Paediatr. 86(10):1997;1040-1041.
20. Vornov J.J., Tasker R.C., Coyle J.T. Direct observation of the agonist-specific regional vulnerability to glutamate, NMDA, and kainate neurotoxicity in organotypic hippocampal cultures. Exp. Neurol. 114:1991;11-22.
21. Vartanian M.G., Cordon J.J., Kupina N.C., Schielke G.P., Posner A., Raser K.G., Wang K.K., Taylor C.P. Phenytoin pretreatment prevents hypoxic-ischemic brain damage in neonatal rats. Brain Res. 95(2):1996;169-175.
22. Weber M.L., Taylor C.P. Damage from oxygen and glucose deprivation in hippocampal slices is prevented by tetrodotoxin, lidocaine and phenytoin without blockade of action potentials. Brain Res. 664(1-2):1994;167-177.
23. Yager J.Y., Asselin J. Effect of mild hypothermia on cerebral energy metabolism during the evolution of hypoxic-ischemic brain damage in the immature rat. Stroke. 27(5):1996;919-925.