Diluent buffer ions and pH: Their influence on the performance of monoclonal antibodies in immunohistochemistry

Applied Immunohistochemistry

Volumn 7, Issue 4, 1999, Pages 300-306

  Boenisch, Thomas ^a  

^a AGILENT TECHNOLOGIES   (United States)

Author keywords

Diluent buffers; Immunohistochemistry; Monoclonal antibodies

Indexed keywords

MONOCLONAL ANTIBODY;

ANTIGEN ANTIBODY REACTION; ARTICLE; HUMAN; HUMAN TISSUE; IMMUNOHISTOCHEMISTRY; ION TRANSPORT; MOLECULAR INTERACTION; PH MEASUREMENT; PRIORITY JOURNAL;

EID: 0032806443     PISSN: 10623345     EISSN: None     Source Type: Journal    
DOI: 10.1097/00022744-199912000-00009     Document Type: Article

References (16)

1. Larsson L-I. Section pretreatment, epitope demasking, and methods for dealing with unwanted staining. In: Larsson L-L, ed. Immunocytochemistry: theory and practice. Boca Raton: CRC Press, 1988:147-70.
2. Taylor CR, Shi S-R, Cote RJ. Antigen retrieval for immunohistochemistry: status and need for greater standardization. Appl Immunohistochem 1996;4:144-66.
3. Leong S-Y, Milios J, Leong FJ. Epitope retrieval with microwaves: a comparison of citrate buffer and EDTA with three commercial retrieval solutions. Appl Immunohistochem 1996;4:201-7.
4. Milstein C. Overview: monoclonal antibodies. In: Weir DM, ed. Handbook of experimental immunology: applications of immunological methods in biomedical sciences. Oxford: Blackwell Scientific Publications, 1986:107.1-107.12.
5. Seppaelae I, Sarvas H, Peterfy F, Maekelae O. The four subclasses of IgG can be isolated from mouse serum by using protein A-Sepharose. Scand J Immunol 1981;14:335-42.
6. Howard AN, Virella G. The separation of pooled human IgG into fractions by isoelectric focusing, and their electrophoretic and immunological properties. In: Peeters H, ed. Protides of the biological fluids. Oxford: Pergamon Press, 1979:449-61.
7. Roit I. 4. The recognition of antigen. In: Essential immunology. London: Blackwell Scientific Publications, 1991:64-83.
8. van Oss CJ. Methods of immunoglobulin isolation and characterization. In: Atassi MZ, van Oss CJ, Absalom DR, eds. Molecular immunology. New York: Marcel Decker, 1984:281-304.
9. van Oss CJ, Absalom DR. Nature and thermodynamics of antigen-antibody interactions. In: Atassi MZ, van Oss CJ, Absalom DR, eds. Molecular immunology. New York: Marcel Decker, 1984: 337-60.
10. van Oss CJ. Agglutination and precipitation. In: Atassi MZ, van Oss CJ, Absalom DR, eds. Molecular immunology. New York: Marcel Decker, 1984:361-79.
11. Calvanico NJ. Structure and function of immunoglobulins. In: Atassi MZ, van Oss CJ, Absalom DR, eds. Molecular immunology. New York: Marcel Decker, 1984:141-74.
12. Bankert RB, Jouy H, Mayers GL. Monoclonal antibodies/myelomas and hybridomas. In: Atassi MZ, van Oss CJ, Absalom DR, eds. Molecular immunology. New York: Marcel Decker, 1984: 175-200.
13. Reimer HM, Wick MR. Theoretical and technical considerations for the use of monoclonal antibodies in diagnostic immunohistochemistry. In: Wick MR, Siegal GP, eds. Monoclonal antibodies in diagnostic immunohistochemistry. New York: Marcel Decker, 1988:1-30.
14. Boenisch T. Background. In: Naish SJ, ed. Handbook: immunochemical staining methods. Carpinteria: DAKO, 1989:21-3.
15. Mosmann TR, Gallatin G, Longenecker BM. Alteration of apparent specificity of monoclonal (hybridoma) antibodies recognizing polymorphic histocompatibility and blood group determinants. J Immunol 1980;125:1152-6.
16. Zola H. Speaking personally: monoclonal antibodies as diagnostic reagents. Pathology 1985;17:53-6.