High level expression of soybean trypsin inhibitor gene in transgenic tobacco plants failed to confer resistance against damage caused by Helicoverpa armigera

Journal of Biosciences

Volumn 24, Issue 4, 1999, Pages 445-452

  Nandi, Ashis Kumar ^a,c   Basu, Debabrata ^a   Das, Sampa ^a,b   Sen, Soumitra K ^a  

^a INDIAN INSTITUTE OF TECHNOLOGY   (India)

^b BOSE INSTITUTE   (India)

^c INDIAN INSTITUTE OF SCIENCE   (India)

Author keywords

Heterologous gene expression; Insect resistance; Protease inhibitors; Transgenic plant

Indexed keywords

PLASMID DNA; SOYBEAN TRYPSIN INHIBITOR; TRYPSIN; TRYPSIN INHIBITOR;

GENETICALLY MODIFIED ORGANISM; PERFORMANCE ASSESSMENT; PEST CONTROL; PEST RESISTANCE;

ANIMAL; ANIMAL EXPERIMENT; ARTICLE; EXTRACTION; GENE EXPRESSION; HELICOVERPA ARMIGERA; INSECT; INSECT CONTROL; INSECTICIDE RESISTANCE; NONHUMAN; PROTEIN DEGRADATION; SOYBEAN; TOBACCO; TRANSGENIC PLANT;

GLYCINE MAX; HELICOVERPA ARMIGERA; LEPIDOPTERA; NOCTUIDAE;

EID: 0032806319     PISSN: 02505991     EISSN: None     Source Type: Journal    
DOI: 10.1007/BF02942655     Document Type: Article

References (40)

1. Birk Y 1961 Purification and some properties of a highly active inhibitor of trypsin and α-chymotrypsin from soybean; Biochem. Biophys. Acta 54 378-380
2. Bradford M M 1976 A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of dye binding; Anal. Biochem. 72 248-254
3. Broadway R M and Duffey S 1986 Plant protease inhibitors: Mechanism of action and effect on the growth and digestive physiology of larval Helicoverpa zea and Spodoptera exiqua; J. Insect Physiol. 32 827-833
4. Duan X, Li X, Xue Q, Abo-el-Saad M, Xu D and Wu R 1996 Transgenic rice plants harboring an introduced potato proteinase inhibitor II gene are insect resistant; Nature Biotechnol. 14 494-498
5. Gatehouse A M R, Gatehouse J A and Boulter D 1980 Isolation and characterization of trypsin inhibitor from cowpea (Vigna unguiculata); Phytochemistry 19 751-756
6. Gennis L S and Cantor C R 1976 Double headed protease inhibitors from black eyed peas III: subunit interaction of the native and half site chemically modified proteins; J. Biol. Chem. 251 747-753
7. Green T and Ryan C A 1972 Wound induced proteinase inhibitor in plant leaves: a possible defense mechanism against insects; Science 175 776-777
8. Hilder V A, Gatehouse A M R, Sheerman S E, Barker R F and Boulter D 1986 A novel mechanism of insect resistance engineered into tobacco; Nature (London) 330 160-163
9. Hofgen R and Willmitzer L 1988 Storage of competent cell for Agrobacterium transformation. Nucleic Acids Res. 16 9877
10. Horsch R B, Fry J, Hoffman N L, Wallroth M, Echholtz D, Rogers S G and Fraley R T 1985 A simple and general method for transferring genes into plants; Science 221 1229-1231
11. Irie K, Hosoyama H, Takeuchi T, Iwabuchi K, Watanabe H, Abe M, Abe K and Arai S 1996 Transgenic rice established to express corn cystatin exhibits strong inhibitory against insect gut protease: Plant Mol. Biol. 30 149-157
12. Jefferson R A, Kavanagh T A and Bevan M W 1987 Gus fusion: β-glucuronidase as a sensitive and versatile gene fusion marker in higher plants; EMBO J. 6 3901-3907
13. Jofuku K D and Goldberg R B 1989 Kunitz trypsin inhibitor gene are differentially expressed during the soybean life cycle and in transformed tobacco plants; Plant Cell 1 1079-1093
14. Jofuku K D, Schipper R D and Goldberg R B 1989 A frameshift mutation prevents Kunitz trypsin inhibitor mRNA accumulation in soybean embryos; Plant Cell 1 427-435
15. Johnson R, Narvaez J, An G and Ryan C 1989 Expression of proteinase inhibitors I and II in transgenic tobacco plant: effects on natural defense against Manduca sexta larvae; Proc. Natl. Acad. Sci. USA 86 9871-9875
16. Johnston K A, Gatehouse J A and Anstee J H 1991 In vitro and in vivo studies of the effects of plant proteinase inhibitors on Helicoverpa armigera larvae; J. Exp. Bot. 42 238
17. Jongsma M A, Bakker P L, Peters J, Bosch D and Stiekema W 1995 Adaptation of Spodoptera exiqua larvae to plant proteinase inhibitors by induction of gut proteinase activity insensitive to inhibition; Proc. Natl. Acad. Sci. USA 92 8041-8045
18. Jotwani M G and Butani D K 1984 Insect pest of crops; in Handbook of agriculture (eds) P L Jaiswal and A M Wadhwani (New Delhi: ICAR) pp 417-550
19. Kay E 1976 Origin of circular dichroism hands in Bowman-Birk soybean trypsin inhibitor; J. Biol. Chem. 251 3411-3416
20. c; J. Biochem. 98 435-448
21. Kunitz M 1947 Isolation of a crystalline protein compound of trypsin and soybean trypsin inhibitor; J. Gen. Physiol. 30 311-320
22. Laemmli U K 1970 Cleavage of structural proteins during the assembly of the head of bacteriophage T4; Nature (London) 227 680-685
23. Laskowski M and Sealock R W 1978 The Enzymes vol 3 (New York: Academic Press)
24. Lee S L, Lee S-Ho, Koo J C, Chun H J, Lim C O, Mun H J and Song Y H 1999 Soybean kunitz trypsin inhibitor confers resistance to the brown plant hopper in transgenic rice; Mol. Breeding 5 1-9
25. Malehorn D E, Borgmeyer J R, Smith C E and Shah D M 1994 Characterization and expression of an anti fungal zeamatin like protein from Zea mays; Plant Physiol. 106 1471-1481
26. Murashige T and Skoog F 1962 A revised medium for rapid growth and bio-assays with tobacco tissue cultures; Physiol. Plant 15 473-497
27. Purcell J P, Greenplate J T and Sammons R D 1992 Examination of midgut luminal proteinase activities in six economically important insects; Insect Biochem. Mol. Biol. 22 41-47
28. Pusztai A, Grant G, Brown D J, Stewart J C, Bardocz S, Ewen S W, Gatehouse A M and Hilder V 1992 Nutritional evaluation of the trypsin (EC 3.4.21.4) inhibitor from cowpea (Vigna unguiculata Walp.); Br. J. Nutr. 68 783-791
29. Rogers S O and Benedich A J 1988 Extraction of DNA from plant tissues; in Plant molecular biology (eds) S B Gelvin, R A Schilperoort and D P S Verma (Netherlands: Kluwer Academic Publishers) pp A6: 1-10
30. Sambrook J, Fritsch E F and Maniatis T 1989 Molecular cloning; A laboratory manual 2nd edition (New York: Cold Spring Harbor Laboratory)
31. Sanger F, Nicklen S and Coulson A R 1977 DNA sequencing with chain terminator inhibitors; Proc. Natl. Acad. Sci. USA 74 5463-5466
32. Schuler T H, Poppy G M, Kerry B R and Denholm I 1998 Insect resistant transgenic plants; Trend. Biotech. 16 168-175
33. Shagger H and Jagow G V 1987 Tricine sodium dodecyl sulphate polyacrylamide gel electrophoresis for the separation of proteins in range from 1 to 100 kDa; Anal. Biochem. 166 368-379
34. Spies R J 1957 Colorimetric procedures for aminoacids; Methods Enzymol. 3 467-477
35. Sogawa K 1970 Studies on feeding habits of the brown plant hopper I. Effects of nitrogen deficiency of host plant insect feeding; Jpn. J. Appl. Entomol. Zool. 14 101-106
36. Southern E M 1975 Detection of specific sequences among DNA fragments separated by gel electrophoresis; J. Mol. Biol. 96 503
37. Towbin H, Staehclin T and Goedon J 1979 Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets; Proc. Natl. Acad. Sci. USA 76 4350-4354
38. Wei C H 1983 Crystalization of two cubic forms of soybean trypsin inhibitor E-1, a member of Bowman-Birk family; J. Biol. Chem. 258 9357-9359
39. Wu Y, Llewellyn D, Mathews A and Dennis E S 1997 Adaptation of Helicoverpa armigera to a proteinase inhibitor expressed in transgenic tobacco; Mol. Breeding 3 371-380
40. Xu D, Xue Q, McElroy D, Mawal Y, Hilder V A and Wu R 1996 Constitutive expression of cowpea trypsin inhibitor gene CPTI in transgenic rice plants confer resistance to two major rice insect pests; Mol. Breeding 2 167-173