Involvement of small GTPases Rho and Rac in the invasion of rat ascites hepatoma cells

Clinical and Experimental Metastasis

Volumn 17, Issue 2, 1999, Pages 141-148

  Imamura, Fumio ^a   Mukai, Mutsuko ^b   Ayaki, Masako ^b   Takemura, Koichi ^c   Horai, Takeshi ^a   Shinkai, Kiyoko ^b   Nakamura, Hiroyuki ^b   Akedo, Hitoshi ^b  

^a Osaka Medical Center for Cancer and Cardiovascular   (Japan)

^b Osaka Medical College   (Japan)

^c OSAKA MEDICAL COLLEGE   (Japan)

Author keywords

Cell surface blebbing; Invasion; Pseudopodia; Rac; Rho

Indexed keywords

BACTERIAL ENZYME; BOTULINUM TOXIN; COMPLEMENTARY DNA; GENISTEIN; GUANOSINE TRIPHOSPHATASE; LYSOPHOSPHATIDIC ACID; PROTEIN TYROSINE KINASE;

ANIMAL CELL; ARTICLE; ASCITES HEPATOMA; CANCER INHIBITION; CANCER INVASION; CELL STRUCTURE; DRUG STRUCTURE; HEPATOMA CELL; LUNG CANCER; MEMBRANE VESICLE; MESOTHELIUM CELL; METASTASIS; MORPHOLOGY; NONHUMAN; PSEUDOPODIUM; RAT; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

ANIMALS; ASCITIC FLUID; BLOTTING, WESTERN; CLOSTRIDIUM BOTULINUM; EPITHELIUM; GENISTEIN; GTP PHOSPHOHYDROLASES; GTPASE-ACTIVATING PROTEINS; LIVER NEOPLASMS, EXPERIMENTAL; LYSOPHOSPHOLIPIDS; MICROSCOPY; NEOPLASM INVASIVENESS; PROTEIN-TYROSINE KINASES; PROTEINS; PSEUDOPODIA; RATS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RHO GTP-BINDING PROTEINS; TRANSFECTION; TUMOR CELLS, CULTURED;

EID: 0032806138     PISSN: 02620898     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1006598531238     Document Type: Article

References (22)

1. Habets GG, Scholtes EH, Zuydgeest D, van der Kammer RA, Stain JC, Berns A, Collard JG. Identification of an invasion-inducing gene gene, Tiam-1, that encodes a protein with a homology of GDP-GTP exchangers for Rho-like proteins. Cell 1995; 77: 537-49.
2. Michels F, Habets GG, Stain JC, van der Kammer RA, Collard JG. A role for Rac in Tiam1-induced membrane ruffling and invasion. Nature 1995; 375: 338-40.
3. Keely PJ, Westwick JK, Whitehead IP, Der CJ, Parise LV. Cdc42 and Rac1 induce integrin-mediated cell motility and invasiveness through PI 3-kinase. Nature 1997; 390: 632-36.
4. Shaw LM, Rabinovitz I, Wang HH, Toker A, Mercurio AM. Activation of phosphoinositide 3-OH kinase by the alpha6beta4 integrin promotes carcinoma invasion. Cell 1997; 91: 949-60.
5. Takaishi K, Sasaki T, Kato M, Yamochi W, Kuroda S, Nakamura T, Takeichi M, Takai T. Involvement of rho p21 small GTP-binding protein and its regulator in the HGF-induced cell motility. Oncogene 1994; 9: 273-79.
6. Akedo H, Shinkai K, Mukai M, Mori Y, Tateishi R, Tanaka K, Yamamoto R, Morishita T. Interaction of rat ascites hepatoma cells with cultured mesothelial cell monolayer: a model for cancer invasion. Cancer Res 1986; 46: 2416-22.
7. Imamura F, Horai T, Mukai M, Shinkai K, Akedo H. Serum requirement for in vitro invasion by tumor cells. Jpn J Cancer Res 1991; 83: 493-96.
8. Imamura F, Horai T, Mukai M, Shinkai K, Sawada M, Akedo H. Induction of in vitro tumor cells invasion by lysophosphatidic acid or phospholipase D. Biochem Biophys Res Comm 1993; 193: 497-503.
9. Moolenaar WH. Lysophosphatidic acid: G-protein signalling and cellular responses. Curr Opin Cell Biol 1997; 9: 168-73.
10. Imamura T, Shinkai K, Mukai M, Yoshioka K, Komagome R, Iwasaki T, Akedo H. Rho-mediated protein tyrosine phosphorylation in lysophosphatidic-acid-induced tumor-cell invasion. Int J Cancer 1996; 65: 627-32.
11. Yoshioka K, Matsumura F, Akedo K, Itoh K. Small GTP-binding protein Rho stimulates the actomyosin system, leading to invasion of minor cells. J Biol Chem 1998; 273: 5146-54.
12. Yoshioka K, Imamura F, Shinkai K, Miyoshi J, Ogawa H, Mukai M, Komagome R, Akedo H. Participation of rhop21 in serum-dependent invasion by rat ascites hepatoma cells. FEBS Lett 1995; 372: 25-28.
13. Stam JC, Michiels F, van der Kammen RA, Moolenaar WH, Collard JG. Invasion of T-lymphoma cells: cooperation between Rho family GTPases and lysophospholipid receptor signaling. EMBO J 1998; 17: 4066-74.
14. Nishiyama T, Sasaki T, Takaishi K, Kato M, Araki K, Matsuura Y, Takai Y. Rac p21 is involved in insulin-induced membrane ruffling and rho p21 is involved in hepatocyte growth factor- and 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced membrane ruffling in KB cells. Mol Cell Biol 1994; 14: 2447-56.
15. Ridley AJ, Comoglio PM, Hall A. Regulation of scatter factor/hepatocyte growth factor response by Ras, Rac, and Rho in MDCK cells. Mol Cell Biol 1995; 15: 1110-12.
16. Mukai M, Shinkai K, Komatsu K, Akedo H. Potentiation of invasive capacity of rat ascites hepatoma cells by transforming growth factor-β. Jpn J Cancer 1989; 80: 107-10.
17. Trinkaus JP. Formation of protrusions of the cell surface during tissue cell movement. Prog Clin Biol Res 1980; 41: 887-906.
18. Cunningham CC. Actin polymerization and intracellular solvent flow in cell surface blebbing. J Cell Biol 1995; 129: 1589-99.
19. Ridley AJ, Hall A. The small GTP-binding protein rho regulates the assembly of focal adhesions and stress fibers in response to growth factors. Cell 1992; 70: 389-99.
20. D'Souza-Schorey C, Boettner B, Van Aelst L. Rac regulates integrin-mediated spreading and increased adhesion of T lymphocytes. Mol Cell Biol 1998; 18(7): 3936-46.
21. Jalink K, van Corven EJ, Hangeveld T, Morii N, Narumiya S, Mollenaar WH. Inhibition of lysophosphatidate- and thrombin-induced neurite retraction and neuronal cell rounding ribosylation of the small GTP-binding protein Rho. J Cell Biol 1994; 126: 801-10.
22. RhoGAP and membrane-protrusive activities at invadopodia. J Biol Chem 1998; 273: 9-12.