Analysis of the peroxisomal Acyl-CoA oxidase gene product from pichia pastoris and determination of its targeting signal

Yeast

Volumn 15, Issue 11, 1999, Pages 1035-1044

  Koller, A ^a   Spong, A P ^a   Luers G H ^a,b   Subramani, S ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b UNIVERSITY OF BONN   (Germany)

Author keywords

Fatty acid oxidation; Peroxisomal targeting signal; Protein import; PTS1

Indexed keywords

ACYL COENZYME A OXIDASE; GENE PRODUCT; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; RECEPTOR;

AMINO ACID SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; MOLECULAR CLONING; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; PEROXISOME; PICHIA PASTORIS; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN LOCALIZATION; PROTEIN TARGETING;

ACYL-COA OXIDASE; AMINO ACID SEQUENCE; ANIMALS; ANTIBODIES, FUNGAL; BASE SEQUENCE; BLOTTING, SOUTHERN; BLOTTING, WESTERN; CLONING, MOLECULAR; DNA PRIMERS; DNA, FUNGAL; FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT; MICROBODIES; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; OXIDOREDUCTASES; PICHIA; PLASMIDS; POLYMERASE CHAIN REACTION; PRECIPITIN TESTS; PROTEIN SORTING SIGNALS; RABBITS; SACCHAROMYCES CEREVISIAE PROTEINS; SEQUENCE ANALYSIS, DNA;

CANDIDA MALTOSA; CANDIDA TROPICALIS; FUNGI; MYXOGASTRIA; PICHIA PASTORIS; SACCHAROMYCES CEREVISIAE; YARROWIA LIPOLYTICA;

EID: 0032805503     PISSN: 0749503X     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0061(199908)15:11<1035::AID-YEA432>3.0.CO;2-1     Document Type: Article

References (23)

1. Aitchison, J. D., Murray, W. W. and Rachubinski, R. A. (1991). The carboxyl-terminal tripeptide Ala-Lys-Ile is essential for targeting Candida tropicalis trifunctional enzyme to yeast peroxisomes. J. Biol. Chem. 266, 23 197-23 203.
2. Aitchison, J. D., Szilard, R. K., Nuttley, W. M. and Rachubinski, R. A. (1992). Antibodies directed against a yeast carboxyl-terminal peroxisomal targeting signal specifically recognize peroxisomal proteins from various yeasts. Yeast 8, 721-734.
3. Dmochoska, A., Dignard, D., Maleszka, R. and Thomas, D. Y. (1990). Structure and transcriptional control of the Saccharomyces cerevisiae POX1 gene encoding acyl-coenzyme A oxidase. Gene 88, 247-252.
4. El-Shabrawi, Y., Noehammer, C. H., Schauer, S., Winter, E. and Hoefler, G. (1997). cDNA cloning and expression of mouse peroxisomal acyl-CoA oxidase. Submission to GENBANK.
5. Elgersma, Y., Elgersma, H. M., Wenzel, T., McCaffery, J. M., Farquhar, M. G. and Subramani, S. (1998). A mobile PTS2 receptor for peroxisomal protein import in Pichia pastoris. J. Cell Biol. 140, 807-820.
6. Faber, K. N., Heyman, J. A. and Subramani, S. (1998). Two AAA family peroxins, PpPex1p and PpPex6p, interact with each other in an ATP-dependent manner and are associated with different subcellular membranous structures distinct from peroxisomes. Mol. Cell Biol. 18, 936-943.
7. Fournier, B., Saudubray, J. M., Benichou, B., Lyonnet, S., Munnich, A., Clevers, H. and Poll-The, B. T. (1994). Large deletion of the peroxisomal acyl-CoA oxidase gene in pseudoneonatal adrenoleukodystrophy. J. Clin. Invest. 94, 526-531.
8. Gaynor, E. C. and Emr, S. D. (1997). COPI-independent anterograde transport: cargo-selective ER-to-Golgi protein transport in yeast COPI mutants. J. Cell Biol. 136, 789-802.
9. Gould, S. J., McCollum, D., Spong, A. P., Heyman, J. A. and Subramani, S. (1992). Development of the yeast Pichia pastoris as a model organism for a genetic and molecular analysis of peroxisome assembly. Yeast 8, 613-628.
10. Hill, D. E., Boulay, R. and Rogers, D. (1988). Complete nucleotide sequence of the peroxisomal acyl CoA oxidase from the alkane-utilizing yeast Candida maltosa. Nucleic Acids Res. 16, 365-366.
11. Lamentschwandtner, G., Brocard, C., Fransen, M., Van Veldhoven, P., Berger, J. and Hartig, A. (1998). The difference in recognition of terminal tripeptides as peroxisomal targeting signal 1 between yeast and human is due to different affinities of their receptor Pex5p to the cognate signal and to residues adjacent to it. J. Biol. Chem. 273, 33 635-33 643.
12. McCollum, D., Monosov, E. and Subramani, S. (1993). The pas8 mutant of Pichia pastoris exhibits the peroxisomal protein import deficiencies of Zellweger syndrome cells-the PAS8 protein binds to the COOH-terminal tripeptide peroxisomal targeting signal, and is a member of the TPR protein family. J. Cell Biol. 121, 761-774.
13. Mitchell, D. A., Marshall, T. K. and Deschenes, R. J. (1993). Vectors for the inducible overexpression of glutathione S-transferase fusion proteins in yeast. Yeast 9, 715-723.
14. Miyazawa, S., Osumi, T., Hashimoto, T., Ohno, K., Miura, S. and Fujiki, Y. (1989). Peroxisome targeting signal of rat liver acyl-coenzyme A oxidase residues at the carboxy terminus. Mol. Cell. Biol. 9, 83-91.
15. Okazaki, K., Takechi, T., Kambara, N., Fukui, S., Kubota, I. and Kamiryo, T. (1986). Two acylcoenzyme A oxidases in peroxisomes of the yeast Candida tropicalis: primary structures deduced from genomic DNA sequence. Proc. Natl Acad. Sci. U S A 83, 1232-1236.
16. Pearson, W. R. and Lipman, D. J. (1988). Improved tools for biological sequence comparison. Proc. Natl Acad. Sci. USA 85, 2444-2448.
17. Sambrook, J., Fritsch, E. F. and Maniatis, T. (1989). Molecular Cloning: A Laboratory Manual, 2nd edn. Cold Spring Harbor Laboratory Press, New York.
18. Small, G. M. and Lazarow, P. B. (1987). Import of the carboxy-terminal portion of acyl-CoA oxidase into peroxisomes of Candida tropicalis. J. Cell Biol. 105, 247-250.
19. Small, G. M., Szabo, L. J. and Lazarow, P. B. (1988). Acyl-CoA oxidase contains two targeting sequences, each of which can mediate protein import into peroxisomes. EMBO J. 7, 1167-1173.
20. Subramani, S. (1998). Components involved in peroxisome import, biogenesis, proliferation, turnover, and movement. Physiol. Rev. 78, 171-188.
21. Varanasi, U., Chu, S., Espinosa, R., LeBeau, M. M. and Reddy, J. K. (1994). Isolation of the human peroxisomal acyl-CoA oxidase gene: organization, promoter analysis, and chromosomal localization. Proc. Natl Acad. Sci. U S A 91, 3107-3111.
22. Wang, H., Le Clainche, A., Le Dall, M.-T., Wache, Y., Pagot, Y., Belin, J.-M., Gaillardin, C. and Nicaud, J.-M. (1998). Cloning and characterization of the peroxisomal acyl CoA oxidase ACO3 gene from the alkane-utilizing yeast Yarrowia lipolytica. Yeast 14, 1373-1386.
23. Zhang, J. W., Han, Y. and Lazarow, P. B. (1993). Novel peroxisome clustering mutants and peroxisome biogenesis mutants of Saccharomyces cerevisiae. J. Cell Biol. 123, 1133-1147.