Alphaxalone activates a Cl- conductance independent of GABA(A) receptors in cultured embryonic human dorsal root ganglion neurons

Journal of Neurophysiology

Volumn 82, Issue 1, 1999, Pages 10-15

  Valeyev, Alexander Y ^a   Hackman, John C ^a   Holohean, Alice M ^a   Wood, Patrick M ^a   Katz, Jennifer L ^a   Davidoff, Robert A ^a  

^a UNIVERSITY OF MIAMI MILLER SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

4 AMINOBUTYRIC ACID A RECEPTOR; ALFAXALONE; BICUCULLINE; CHLORIDE CHANNEL; CHLORIDE ION; PICROTOXIN; TERT BUTYLBICYCLOPHOSPHOROTHIOATE;

ARTICLE; CHLORIDE CONDUCTANCE; CHLORIDE CURRENT; CONCENTRATION RESPONSE; DRUG RECEPTOR BINDING; EMBRYO; HUMAN; HUMAN CELL; NERVE CELL MEMBRANE POTENTIAL; PATCH CLAMP; PRIORITY JOURNAL; RECEPTOR OCCUPANCY; SPINAL CORD NERVE CELL; SPINAL GANGLION;

EID: 0032802324     PISSN: 00223077     EISSN: None     Source Type: Journal    
DOI: 10.1152/jn.1999.82.1.10     Document Type: Article

References (44)

1. AKAIKE, N., HATTORI, K., OOMURI, Y., AND CARPENTER, D. O. Bicuculline and picrotoxin block ganuna-aminobutyric acid-gated Cl-conductance by different mechanisms. Experientia 41: 70-71, 1985.
2. AKAIKE, N., YAKUSHIJI, T., TOKUTOMI, N., AND CARPENTER, D. O. Multiple mechanisms of antagonism of γ-aminobutryric acid (GABA) responses. Cell. Mol. Neurobiol. 1: 97-103, 1987.
3. ALLEN, C. N. AND ALBUQUERQUE, E. X. Conductance properties of GABA-activated chloride currents recorded from cultured hippocampal neurons. Brain Res. 410: 159-163, 1987.
4. BARKER, J. L., HARRISON, N. L., LANGE, G. D., AND OWEN, D. G. Potentiation of γ-aminobutyric acid-activated chloride conductance by a steroid anaesthetic in cultured rat spinal neurones. J. Physiol. (Lond.) 386: 485-501, 1987.
5. BORMANN, J. AND CLAPHAM, D. E. γ-Aminobutyric acid receptor channels in adrenal chromaffin cells: a patch-clamp study. Proc. Natl. Acad. Sci. USA 82: 2168-2172, 1985.
6. A receptor by progesterone metabolites. Proc. R. Soc. Lond. B Biol. Sci. 231: 359-369, 1987.
7. CHESNOY-MARCHAIS, D. Characterization of a chloride conductance activated by hyperpolarization in Aplysia neurones. J. Physiol. (Lond.) 342: 277-308, 1983.
8. COLQUHOUN, D., NEHER, E., REUTER, H., AND STEVENS, C. F. Inward current channels activated by intracellular Ca in cultured cardiac cells. Nature 294: 752-754, 1981.
9. A receptor activity by alphaxalone. Br. J. Pharmacol. 90: 491-500, 1987.
10. CURMI, J. P., PREMKUMAR, L. S., BIRNIR, B., AND GAGE, P. W. The influence of membrane potential on chloride channels activated by GABA in rat cultured hippocampal neurons. J. Membr. Biol. 136: 273-280, 1993.
11. DUNLAP, K. Functional and pharmacological differences between two types of GABA receptors on embryonic chick sensory neurons. Neurosci. Lett. 47: 265-270, 1984.
12. ECCLES, J. C., SCHMIDT, R. F., AND WILLIS, W. D. Pharmacological studies on presynaptic inhibition. J. Physiol. (Lond.) 168: 500-530, 1963.
13. FATIMA-SHAD, K. AND BARRY, P. H. A patch-clamp study of GABA- and strychnine-sensitive glycine-activated currents in post-natal tissue-cultured hippocampal neurones. Proc. R. Soc. Lond. B Biol. Sci. 250: 99-105, 1992.
14. GALLAGHER, J. P., HIGASHI, H., AND NISHI, S. Characterization and ionic basis of GABA-induced depolarization recorded in vitro from cat primary afferent neurones. J. Physiol. (Lond.) 275: 263-282, 1978.
15. GEE, K. W., BOLGER, M. B., BRINTON, R. E., COIRINI, H., AND MCEWEN, B. S. Steroid modulation of the chloride ionophore in rat brain: structure-activity requirements, regional dependence and mechanisms of action. J. Pharmacol. Exp. Ther. 246: 803-812, 1988.
16. GRAY, R. AND JOHNSTON, D. Rectification of single GABA-gated chloride channels in adult hippocampal neurons. J. Neurophysiol. 54: 134-142, 1985.
17. HAMILL, O. P., BORMANN, J., AND SAKMANN, B. Activation of multiple-conductance state chloride channels in spinal neurones by glycine and GABA. Nature 305: 805-808, 1983.
18. HARRISON, N. L. AND SIMMONDS, M. A. Modulation of the GABA receptor complex by a steroid anaesthetic. Brain Res. 323: 287-292, 1984.
19. INOUE, M. AND AKAIKE, N. Blockade of gamma-aminobutyric acid-gated chloride current in frog sensory neurons by picrotoxin. Neurosci. Res. 5: 380-394, 1988.
20. KORN, S. J. AND WEIGHT, F. F. Patch-clamp study of the calcium-dependent chloride current in AtT-20 pituitary cells. J. Neurophysiol. 58: 1431-1451, 1987.
21. A receptor function. Trends Pharmacol. Sci. 16: 295-303, 1995.
22. A receptor main conductance state of mouse spinal cord neurones in culture. J. Physiol. (Lond) 410: 479-499, 1989.
23. MAGER, R., FERRONI, S., AND SCHUBERT, P. GTP- and GDP-analogues modulate an inwardly rectifying chloride channel in cultured hippocampal neurons. Neurosci. Lett. 184: 165-168, 1995.
24. A receptor. Mechanism of action and physiological significance. Prog. Neurobiol. 38: 379-395, 1992.
25. MAYER, M. L. A calcium-activated chloride current generates the after-depolarization of rat sensory neurones in culture. J. Physiol. (Lond.) 364: 217-240, 1985.
26. A receptor of mouse spinal neurones in culture. Exp. Physiol. 75: 199-209, 1990.
27. MISTRY, D. K. AND HABLITZ, J. J. Activation of subconductance states by γ-aminobutyric acid and its analogs in chick cerebral neurons. Pflügers Arch. 416: 454-461, 1990.
28. NEWLAND, C. F. AND CULL-CANDY, S. G. On the mechanism of action of picrotoxin on GABA receptor channels in dissociated sympathetic neurones. J. Physiol. (Lond.) 447: 191-213, 1992.
29. OLSEN, R. W. AND SAPP, D. W. Neuroactive steroid modulation of GABAA receptors. Adv. Bio Chem. Psychopharmacol. 48: 57-74, 1995.
30. ONG, J., KERR, D. I., AND JOHNSTON, G.A.R. Alfaxalone potentiates and mimics GABA-induced contractile responses in the guinea-pig isolated ileum. Br. J. Pharmacol. 95: 33-38, 1988.
31. OZAWA, S. AND YUZAKI, M. Patch-clamp studies of chloride channels activated by gamma-aminobutyric acid in cultured hippocampal neurones of the rat. Neurosci. Res. 1: 275-293, 1984.
32. PARKER, I. AND MILEDI, R. A calcium-independent chloride current activated by hyperpolarization in Xenopus oocyctes. Proc. R. Soc. Lond. B Biol. Sci. 233: 191-199, 1988.
33. PAUL, S. M. AND PURDY, R. H. Neuroactive steroids. FASEB J. 6: 2311-2322, 1992.
34. A receptor by depressant barbiturates and pregnane steroids. Br. J. Pharmacol. 94: 1257-1269, 1988.
35. SEAR, J. W. AND PRYS-ROBERTS, C. Plasma concentrations of alphaxalone during continuous infusion with Althesin. Br. J. Anaesth. 51: 861-865, 1979.
36. SCHULZ, D. W. AND MACDONALD, R. L. Barbiturate enhancement of GABA-mediated inhibition and activation of chloride ion conductance: correlation with anticonvulsant and anesthetic actions. Brain Res. 209: 177-188, 1981.
37. SMITH, S. M., ZOREC, R., AND MCBURNEY, R. N. Conductance states activated by glycine and GABA in rat cultured spinal neurones. J. Membr. Biol. 108: 45-52, 1989.
38. STALEY, K. The role of an inwardly rectifying chloride conductance in postsynaptic inhibition. J. Neurophysiol, 72: 273-284, 1994.
39. TALEB, O., TROUSLARD, J., DEMENEIX, B. A., FELTZ, P., BOSSU, J. L., AND FELTZ, A. Spontaneous and GABA-evoked chloride channels on pituitary intermediate lobe cells and their internal Ca requirement. Pflügers Arch. 409: 620-631, 1987.
40. A receptor single-channel kinetic properties of mouse spinal cord neurons in culture. J. Physiol. (Lond.) 456: 215-245, 1992.
41. - current in cultured embryonic human dorsal root ganglion neurons. J. Neurophysiol. 82: 1-9, 1999.
42. - current activated by alphaxalone in embryonic human DRG cells. Soc. Neurosci. Abstr. 22: 1026, 1996.
43. WEISS, D. S., BARNES, E. M., AND HABLITZ, J. J. Whole cell and single-channel recordings of GABA-gated currents in cultured chick cerebral neurons. J. Neurophysiol. 59: 495-513, 1988.
44. 2+-activated nonselective cation channels in neuroblastoma. Nature 296: 357-359, 1982.