The mycoparasite Ampelomyces quisqualis expresses exgA encoding an exo-β-1,3-glucanase in culture and during mycoparasitism

Phytopathology

Volumn 89, Issue 8, 1999, Pages 631-638

  Rotem, Yaniv ^a   Yarden, Oded ^a   Sztejnberg, Abraham ^a  

^a HEBREW UNIVERSITY OF JERUSALEM   (Israel)

Author keywords

Lytic enzymes

Indexed keywords

1,3 BETA GLUCANASE; FUNGAL GENETICS; GENE EXPRESSION; PATHOGENESIS; POWDERY MILDEW;

AMPELOMYCES QUISQUALIS; COCHLIOBOLUS CARBONUM; GLIOCLADIUM ROSEUM; NEUROSPORA CRASSA; SPHAEROTHECA FUSCA; TRICHODERMA HARZIANUM;

EID: 0032801449     PISSN: 0031949X     EISSN: None     Source Type: Journal    
DOI: 10.1094/PHYTO.1999.89.8.631     Document Type: Article

References (21)

1. Abo-Foul, S., Raskin, V. I., Sztejnberg, A., and Marder, J. B. 1996. Disruption of chlorophyll organization and function in powdery mildew-diseased cucumber leaves and its control by the hyperparasite Ampelomyces quisqualis. Phytopathology 86:195-199.
2. Beth Din, A., and Yarden, O. 1994. The Neurospora crassa chs-2 gene encodes a non-essential chitin synthase. Microbiology 140:2189-2197.
3. Bruchez, J. J. P., Eberle, J., and Russo, V. E. A. 1993. Regulatory sequences involved in the translation of Neurospora crassa mRNA: Kozak sequences and stop codons. Fungal Genet. Newsl. 40:85-88.
4. Chet, I., Inbar, J., and Hadar, Y. 1997. Fungal antagonism and mycoparasitism. Pages 165-184 in: The Mycota. Vol. 4, Environmental and Microbial Relationships. K. Esser and P. A. Lemke, eds. Springer-Verlag, Berlin.
5. Chiu, S. C., and Tzean, S. S. 1995. Glucanolytic enzyme production by Schizophyllum commune Fr. during mycoparasitism. Physiol. Mol. Plant Pathol. 46:83-94.
6. De La Cruz, J., Pintor-Toro, J. A., Benitez, T, Llobell, A., and Romero, L. C. 1995. Anovel endo-β-1,3-glucanase, BNG13.1, involved in mycoparasitism of Trichoderma harzianum. J. Bacteriol. 177:6937-6945.
7. Elad, Y., Chet, I., Boyle, P., and Henis, Y. 1983. Parasitism of Trichoderma spp. on Rhizoctonia solani and Sclerotium rolfsii - Scanning electron microscopy and fluorescence microscopy. Phytopathology 73:85-88.
8. Falk, S. P., Gadoury, D. M., Cortesi, P., Pearson, R. C., and Seem, R. C. 1995. Parasitism of Uncinula necator cleistothecia by the mycoparasite Ampelomyces quisqualis. Phytopathology 85:794-800.
9. Hashioka, Y., and Nakai, Y. 1980. Ultrastructure of pycnidial development and mycoparasitism of Ampelomyces quisqualis parasitic on Erysiphales. Trans. Mycol. Soc. Jpn. 21:329-338.
10. Larena, I., and Melgarejo, P. 1993. The lytic enzymatic complex of Penicillium purpurogenum and its effects on Monilinia laxa. Mycol. Res. 97: 105-110.
11. Mackenzie, L. F., Brooke, G. S., Cutfield, J. F., Sullivan, P. A., and Withers, S. G. 1997. Identification of Glu-330 as the catalytic nucleophile of Candida albicans exo-beta-(1,3)-glucanase. J. Biol. Chem. 272:3161-3167.
12. Philipp, W. D. 1985. Extracellular enzymes and nutritional physiology of Ampelomyces quisqualis Ces., hyperparasite of powdery mildew, in vitro. Phytopathol. Z. 114:274-283.
13. Pitson, S. M., Seviour, R. J., and McDougall, B. M. 1993. Noncellulolytic fungal β-glucanases: Their physiology and regulation. Enzyme Microb. Technol. 15:178-192.
14. Sambrook, J., Fritsch, E. F., and Maniatis, T. 1989. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
15. Schaeffer, H. J., Leykam, J., and Walton, J. D. 1994. Cloning and targeted gene disruption of EXG1, encoding exo-β-1,3-glucanase, in the phytopathogenic fungus Cochliobolus carbonum. Appl. Environ. Microbiol 60:594-598.
16. Sokolovsky, V., Kaldenhoff, R., Ricci, M., and Russo, V. E. A. 1990. Fast and reliable mini-prep RNA extraction from Neurospora crassa. Fungal Genet. Newsl. 37:41-43.
17. Sztejnberg, A., Galper, S., and Lisker, N. 1989. Conditions for pycnidial production and spore formation by Ampelomyces quisqualis. Can. J. Microbiol. 36:193-198.
18. Tweddell, R. J., Jabaji-Hare, S. H., Goetghebeur, M., Charest, P. M., and Kermasha, S. 1995. Purification and partial characterization of a β-1,3-glucanase secreted by the mycoparasite Stachybotrys elegans. Biosci. Biotechnol. Biochem. 59:2223-2227.
19. Urquhart, E. J., Menzies, J. S., and Punja, Z. K. 1994. Growth and biological control activity of Tilletiopsis species against powdery mildew (Sphaerotheca fuliginea) on greenhouse cucumber. Phytopathology 84:341-351.
20. Vogel, H. J. 1956. A convenient growth medium for Neurospora crassa (medium N). Microb. Genet. Bull. 13:42-43.
21. Weiss, N., Sztejnberg, A., and Yarden, O. 1996. The chsA gene, encoding a class-I chitin synthase from Ampelomyces quisqualis. Gene 168:99-102.