Adsorption of enzymes onto submicron-sized temperature-sensitive composite polymer particles and its activity

Colloids and Surfaces A: Physicochemical and Engineering Aspects

Volumn 153, Issue 1-3, 1999, Pages 429-433

  Okubo, M ^a   Ahmad, H ^a  

^a KOBE UNIVERSITY   (Japan)

Author keywords

Adsorption; Morphology; Specific activity; Temperature sensitive; Trypsin

Indexed keywords

ADSORPTION; CONFORMATIONS; COPOLYMERIZATION; DESORPTION; ENZYMES; MOLECULES; POLYSTYRENES; SPECTROSCOPY; SURFACES; TEMPERATURE;

BIOMOLECULES; CIRCULAR DICHROISM SPECTROSCOPY; DIMETHYLAMINOETHYL METHACRYLATE; ENZYMATIC ACTIVITY; ETHYLENE GLYCOL DIMETHACRYLATE; LOWER CRITICAL SOLUTION TEMPERATURE; SEEDED EMULSION COPOLYMERIZATION; TEMPERATURE SENSITIVE COMPOSITE POLYMER PARTICLES; TRYPSIN;

PARTICLES (PARTICULATE MATTER);

COPOLYMER; POLYSTYRENE; RESIN; TRYPSIN;

ADSORPTION; CIRCULAR DICHROISM; CONFERENCE PAPER; CONFORMATIONAL TRANSITION; ENZYME ACTIVITY; ENZYME IMMOBILIZATION; IN VITRO STUDY; PRIORITY JOURNAL; TEMPERATURE;

EID: 0032799497     PISSN: 09277757     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0927-7757(98)00464-6     Document Type: Conference Paper

References (10)

1. Okubo M., Ahmad H. Colloid Polym. Sci. 273:1995;817.
2. Kamei S., Okubo M., Matsuda T., Matsumoto T. Colloid Polym. Sci. 264:1986;743.
3. Tan J.S., Martic P.A. J. Colloid Interface Sci. 136:1990;415.
4. Lu D.R., Park K. J. Colloid Interface Sci. 144:1991;272.
5. Laskowski M. Methods Enzymol. 2:1955;26.
6. Okubo M., Ahmad H. Colloid Polym. Sci. 274:1995;112.
7. A. Wiseman, Handbook of Enzyme Biotechnology, John Wiley and Sons, New York, 1985.
8. Filippusson H., Hornby W.E. Biochem. J. 120:1970;215.
9. Francesco R.D., Pastore A., Vecchio G., Cortese R. Biochemistry. 30:1991;143.
10. Kamatari Y.O., Konno T., Kataoka M., Akasaka K. J. Mol. Biol. 259:1996;512.