Preparation of nuclear extracts from myelinating Schwann cells

Journal of Neuroscience Methods

Volumn 89, Issue 2, 1999, Pages 129-132

  Forghani, R ^a   Nesbitt, J ^b   Snipes, J ^a   Shooter, E M ^b   Peterson, A ^a  

^a MCGILL UNIVERSITY   (Canada)

^b STANFORD UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

KROX 20; Myelin; Nuclear extracts; Schwann cells; Transcription factors

Indexed keywords

CELL EXTRACT; KROX 20; MYELIN; TRANSCRIPTION FACTOR; UNCLASSIFIED DRUG;

ANIMAL TISSUE; ARTICLE; BINDING SITE; CELL NUCLEUS; CONTROLLED STUDY; ELECTROPHORETIC MOBILITY; GENE EXPRESSION; MOLECULAR BIOLOGY; MOUSE; MYELINATION; NEWBORN; NONHUMAN; OLIGONUCLEOTIDE PROBE; PERIPHERAL NERVE; PRIORITY JOURNAL; SCHWANN CELL; SCIATIC NERVE; SIGNAL TRANSDUCTION; TECHNIQUE;

ANIMALS; ANIMALS, NEWBORN; GENE EXPRESSION REGULATION; MICE; MYELIN SHEATH; NUCLEAR PROTEINS; SCHWANN CELLS; SCIATIC NERVE; TRANSCRIPTION FACTORS;

EID: 0032798789     PISSN: 01650270     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0165-0270(99)00043-6     Document Type: Article

References (22)

1. Chavrier P., Vesque C., Galliot B., Vigneron M., Dolle P., Duboule D., Charnay P. The segment-specific gene Krox-20 encodes a transcription factor with binding sites in the promoter region of the Hox-1.4 gene. EMBO J. 9:1990;1209-1218.
2. Chavrier P., Zerial M., Lemaire P., Almendral J., Bravo R., Charnay P. A gene encoding a protein with zinc fingers is activated during G0/G1 transition in cultured cells. EMBO J. 7:1988;29-35.
3. Dignam J.D., Lebovitz R.M., Roeder R.G. Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei. Nucl. Acids Res. 11:1983;1475-1489.
4. Dignam J.D., Martin P.L., Shastry B.S., Roeder R.G. Eukaryotic gene transcription with purified components. Methods Enzymol. 101:1983;582-598.
5. Eyer J., Peterson A.C. Neurofilament-deficient axons and perikaryal aggregates in viable transgenic mice expressing a neurofilament-ß-galactosidase fusion protein. Neuron. 12:1994;389-405.
6. Foran D.R., Peterson A.C. Myelin acquisition in the central nervous system of the mouse revealed by an MBP-Lac Z transgene. J. Neurosci. 12:1992;4890-4897.
7. Goujet-Zalc C., Babinet C., Monge M., Timsit S., Cabon F., Gansmuller A., Miura M., Sanchez M., Pournin S., Mikoshiba K., Zalc B. The proximal region of the MBP gene promoter is sufficient to induce oligodendroglial-specific expression in transgenic mice. Eur. J. Neurosci. 5:1993;624-632.
8. Gow A., Friedrich V.L. Jr., Lazzarini R.A. Myelin basic protein gene contains separate enhancers for oligodendrocyte and Schwann cell expression. J. Cell. Biol. 119:1992;605-616.
9. He X., Gerrero R., Simmons D.M., Park R.E., Lin C.J., Swanson L.W., Rosenfeld M.G. Tst-1, a member of the POU domain gene family, binds the promoter of the gene encoding the cell surface adhesion molecule P0. Mol. Cell. Biol. 11:1991;1739-1744.
10. Hoppe-Seyler F., Butz K., Rittmuller C., von Knebel Doeberitz M. A rapid microscale procedure for the simultaneous preparation of cytoplasmic RNA, nuclear DNA binding proteins and enzymatically active luciferase extracts. Nucl. Acids Res. 19:1991;5080.
11. Joseph L.J., Le Beau M.M., Jamieson G.A. Jr., Acharya S., Shows T.B., Rowley J.D., Sukhatme V.P. Molecular cloning, sequencing, and mapping of EGR2, a human early growth response gene encoding a protein with 'zinc-binding finger' structure [published erratum appears in Proc Natl Acad Sci USA 1989;86(2):515]. Proc. Natl. Acad. Sci. USA. 85:1988;7164-7168.
12. Lemke G. Unwrapping the genes of myelin. Neuron. 1:1988;535-543.
13. Lemke G., Chao M. Axons regulate Schwann cell expression of the major myelin and NGF receptor genes. Development. 102:1988;499-504.
14. Messing A., Behringer R.R., Hammang J.P., Palmiter R.D., Brinster R.L., Lemke G. P0 promoter directs expression of reporter and toxin genes to Schwann cells of transgenic mice. Neuron. 8:1992;507-520.
15. Monuki E.S., Kuhn R., Lemke G. Repression of the myelin P0 gene by the POU transcription factor SCIP. Mech. Dev. 42:1993;15-32.
16. Monuki E.S., Kuhn R., Weinmaster G., Trapp B.D., Lemke G. Expression and activity of the POU transcription factor SCIP. Science. 249:1990;1300-1303.
17. Monuki E.S., Weinmaster G., Kuhn R., Lemke G. SCIP: a glial POU domain gene regulated by cyclic AMP. Neuron. 3:1989;783-793.
18. Morgan L., Jessen K.R., Mirsky R. The effects of cAMP on differentiation of cultured Schwann cells: progression from and early phenotype (04+) to a myelin phenotype (P0+, GFAP-, N-CAM-, NGF-receptor-) depends on growth inhibition. J. Cell. Biol. 112:1991;457-467.
19. Nardelli J., Gibson T.J., Vesque C., Charnay P. Base sequence discrimination by zinc-finger DNA-binding domains. Nature. 349:1991;175-178.
20. Peters A, Palay LS, Webster HD. The Fine Structure of the Nervous System. 3rd ed. New York: Oxford University Press, 1991.
21. Roy R.J., Gosselin P., Guerin S.L. A short protocol for micro-purification of nuclear proteins from whole animal tissue. Biotechniques. 11:1991;770-777.
22. Topilko P., Schneider-Maunoury S., Levi G., Baron-Van Evercooren A., Chennoufi A.B., Seitanidou T., Babinet C., Charnay P. Krox-20 controls myelination in the peripheral nervous system. Nature. 371:1994;796-799.