Expression, purification, and characterization of interferon-τ produced in Pichia pastoris grown in a minimal medium

Journal of Interferon and Cytokine Research

Volumn 19, Issue 6, 1999, Pages 631-636

  Johnson, Tonny M ^a   Holaday, S Kent ^a   Sun, Yahong ^a   Subramaniam, Prem S ^b   Johnson, Howard M ^b   Krishna, N Rama ^a  

^a UNIVERSITY OF ALABAMA AT BIRMINGHAM   (United States)

^b UNIVERSITY OF FLORIDA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ALPHA INTERFERON; BETA INTERFERON; DODECYL SULFATE SODIUM; GENE PRODUCT; PROTEIN P21; TAU INTERFERON;

ANTIVIRAL ACTIVITY; ARTICLE; CANCER IMMUNOTHERAPY; CIRCULAR DICHROISM; CYTOKINE PRODUCTION; MASS SPECTROMETER; MULTIPLE SCLEROSIS; NONHUMAN; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PICHIA PASTORIS; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN INDUCTION; PROTEIN PURIFICATION; PROTEIN SECONDARY STRUCTURE; VIRUS INFECTION;

ANTIVIRAL AGENTS; CELL DIVISION; CULTURE MEDIA; FEMALE; HUMANS; INTERFERON TYPE I; MAGNETIC RESONANCE SPECTROSCOPY; PICHIA; PREGNANCY; PREGNANCY PROTEINS; RECOMBINANT PROTEINS; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

PICHIA PASTORIS;

EID: 0032798511     PISSN: 10799907     EISSN: None     Source Type: Journal    
DOI: 10.1089/107999099313776     Document Type: Article

References (25)

1. SOOS, J.M., SCHIFFENBAUER, J., JOHNSON, H.M., and PONTZER, C.H. (1997). Interferon-τ: A potential treatment for inflammatory central nervous system disease. In: Interferon Therapy of Multiple Scherosis. A.T. Reder (ed.) New York, NY: Marcel Dekker Inc., pp. 479-498.
2. IMAKAWA, K., ANTHONY, R.V., KAZEMI, M., MAROTTI, K.R., POLITES, G.H., and ROBERTS, R.M. (1987). Interferon-like sequence of ovine trophoblast protein secreted by embryonic trophectoderm. Nature 330, 377-379.
3. ROBERTS, R.M., CROSS, J.C., and LEAMAN, D.W. (1992). Interferons as hormones of pregnancy. Endocr. Rev. 13, 432-452.
4. DEREUDDRE-BOSQUET, N., CLAYETTE, P., MARTIN, M., MABONDZO, A., FRETIER, P., GRAS, G., MARTAL, J., and DORMANT, D. (1996). Anti-HIV potential of a new interferon, interferon-tau (trophoblastin). J. Acquired. Immun. Deficiency Synd. & Human Retrovirology 11, 241-246.
5. JUSTE, R.A., OTT, T.L., KWANG, J., BAZER, F.W., and DELACONCHABERMEJILLO, A. (1996). Effect of recombinant interferon-tau on ovine lentivirus replication. J. Interferon & Cytokine Research 16, 989-994.
6. PONTZER, C.H., YAMAMOTO, J.K., BAZER, F.W., OTT, T.L., and JOHNSON, H.M. (1997). Potent anti-feline immunodeficiency virus effect and anti-human immunodeficiency virus effect of IFN-tau. J. Immunol. 158, 4351-4357.
7. HOBEIKA, A.C. SUBRAMANIAM, P.S., and JOHNSON, H.M. (1997). IFNα induces the expression of the cyclin-dependent kinase inhibitor p21 in human prostate cancer cells. Oncogene 14, 1165-1170.
8. PONTZER, C.H., BAZER, F.W., and JOHNSON, H.M. (1991). Antiproliferative activity of a pregnancy recognition hormone, ovine trophoblast protein-1. Cancer. Res. 51, 5304-5307.
9. SUBRAMANIAM, P.S., and JOHNSON, H.M. (1997). A role for the cyclin-dependent kinase inhibitor p21 in the G1 cell cycle arrest mediated by the type I interferons. J. Interferon Cytokine Res. 17, 11-15.
10. WAF1 is accomplished by ordered G1 arrest, differentiation and apoptosis of the Daudi B-cell line. Oncogene 16, 1885-1890.
11. MUJTABA, M.G., SOOS, J.M., and JOHNSON, H.M. (1997). CD4 T suppressor cells mediated interferon IFNτ protection against experimental allergic encephalomyelitis. J. Neuroimmunol. 75, 35-42.
12. OLDHAM, R.K. (1985). Biologicals for cancer treatment: interferons. Hosp. Pract. 20, 71-91.
13. KHAN, O.A., JIANG, H., SUBRAMANIAM, P.S., JOHNSON, H.M., and DHIB-JALBUT, S.S. (1998). Immunomodulating functions of recombinant ovine interferon tau: potential for therapy in multiple sclerosis and autoimmune disorders. Mult. Scler. 4, 63-69.
14. SUBRAMANIAM, P.S., KHAN, S.A., PONTZER, C.H., and JOHNSON, H.M. (1995). Differential recognition of the type I interferon receptor by interferons τ and α is responsible for their disparate cytotoxicities. Proc. Natl. Acad. Sci. USA 92, 12270-12274.
15. PONTZER, C.H., OTT, T.L., BAZER, F.W., and JOHNSON, H.M. (1990). Localization of an antiviral site on the pregnancy recognition hormone, ovine trophoblast protein 1. Proc. Natl. Acad. Sci. USA 87, 5945-5949.
16. PONTZER, C.H., OTT, T.L., BAZER, F.W., and JOHNSON, H.M. (1994). Structure/function studies with interferon tau: evidence for multiple active sites. J. Interferon Res. 14, 133-141.
17. LI, J., and ROBERTS, R.M. (1994). Structure-function relationships in the interferon-tau (IFN-tau). Changes in receptor binding and in antiviral and antiproliferative activities resulting from site-directed mutagenesis performed near the carboxyl terminus. J. Biol. Chem. 269, 24826-24833.
18. JARPE, M.A., JOHNSON, H.M., BAZER, P.M., OTT, T.L., CURTO, E.V., KRISHNA, N.R., and PONTZER, C.H. (1994). Predicted structural motif of IFN-tau. Protein. Eng. 7, 863-867.
19. SENDA, T., SAITOH, S-I., MITSUI, Y., LI, J., and ROBERTS, R.M. (1995). A three-dimensional model of interferon-τ. J. Interferon Cytokine Res. 15, 1053-1060.
20. VAN HEKEE, G., OTT, T.L., STRAUSS, A., AMMATURO, D., and BAZER, F.W. (1990). High yield expression and secretion of the ovine pregnancy recognition hormone interferon-tau by Pichia pastoris. J. Interferon Cytokine Res. 16, 119-126.
21. BRADFORD, M.M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248-254.
22. FAMILLETTI, P.C., RUBINSTEIN, S., and PESTKA, S.A. (1981). A convenient and rapid cytopathic effect inhibition assay for interferon. Methods. Enzymol. 78, 387-394.
23. PONTZER, C.H., TORRES, B.A., VALLET, J.L., BAZER, F.W., and JOHNSON, H.M. (1988). Antiviral activity of the pregnancy recognition hormone ovine trophoblast protein-1. Biochem. Biophys. Res. Commun. 152, 801-807.
24. BODENHAUSEN, G., and RUBEN, D.J. (1980). Natural Abundance Nitrogen-15 NMR by Enhanced Heternuclear Spectroscopy. Chem. Phys. Lett. 69, 185-189.
25. WISHART, D., SYKES, B., and RICHARDS, F. (1991). Relationship between nuclear magnetic resonance chemical shift and protein secondary structure. J. Mol. Biol. 222, 311-333.