Characterisation and assay of five enzymatic activities in the stratum corneum using tape-strippings

Skin Pharmacology and Applied Skin Physiology

Volumn 12, Issue 4, 1999, Pages 182-192

  Redoules, Daniel ^a   Tarroux, Roger ^a   Assalit, Marie France ^a   Perié, Jean Jacques ^b  

^a INSTITUT DE RECHERCHE PIERRE FABRE   (France)

^b UNIVERSITÉ DE TOULOUSE   (France)

Author keywords

Atopic dermatitis; Epidermal enzymes; Inhibitors; Permeability barrier; pH; Spectrometric methods; Stratum corneum

Indexed keywords

ACID PHOSPHATASE; CHYMOTRYPSIN; GLUCOSYLCERAMIDASE; PHOSPHOLIPASE A2; SERINE PROTEINASE; TRYPSIN;

ADOLESCENT; ADULT; ARTICLE; ATOPIC DERMATITIS; CHILD; CLINICAL ARTICLE; CONTROLLED STUDY; ENZYME ACTIVITY; FEMALE; HUMAN; MALE; PERMEABILITY BARRIER; PH; PRIORITY JOURNAL; SCHOOL CHILD; STRATUM CORNEUM;

ACID PHOSPHATASE; ADOLESCENT; ADULT; CHILD; CHYMOTRYPSIN; DERMATITIS, ATOPIC; ENZYME INHIBITORS; ENZYMES; FEMALE; GLUCOSYLCERAMIDASE; HUMANS; HYDROGEN-ION CONCENTRATION; HYDROLYSIS; INDICATORS AND REAGENTS; MALE; MIDDLE AGED; PHOSPHOLIPASES A; PROTEINS; SKIN; SPECIMEN HANDLING; TRYPSIN;

EID: 0032797223     PISSN: 14222868     EISSN: None     Source Type: Journal    
DOI: 10.1159/000066242     Document Type: Article

References (37)

1. Elias PM, Friend DS: The permeability barrier in mammalian epidermis. J Cell Biol 1975;65:185-191.
2. Matoltsy AG, Balsamo CA: A study of cornified epithelium of human skin. J Biophys Biochem Cytol 1955;1:339-360.
3. Hendriks W, Brugman C, Richter KH, Vanhooijdonk C, Schepens J, Schalkwijk J, Wieringa B: Proteintyrosine phosphatases expressed in mouse epidermal keratinocytes. J Invest Dermatol 1996;106:972-976.
4. Dale BA, Scofield JA, Hennings H, Stanley JR, Yuspa SH: Identification of filaggrin in cultured mouse keratinocytes and its regulation by calcium. J Invest Dermatol 1983;81: 90S-95S.
5. Freinkel R, Traczyk N: Lipid composition and acid hydrolase content of lamellar granules of fetal rat epidermis. J Invest Dermatol 1985;85: 295-298.
6. Holleran WM, Feingold KR, Mao-Qiang M, Gao WN, Lee JM, Elias PM: Regulation of epidermal sphingolipid synthesis by permeability barrier function. J Lipid Res 1991; 32:1151-1158.
7. Holleran WM, Takagi Y, Imokawa G, Jackson SM, Lee JM, Elias PM: β-Glucocerebrosidase activity in murine epidermis: Characterization and localization in relationship to differentiation. J Lipid Res 1992;33: 1201-1209.
8. Mao-Qiang M, Feingold KR, Jain M, Elias PM: Extracellular processing of phospholipids is required for permeability barrier homeostasis. J Lipid Res 1995;36:1925-1935.
9. 2 activity is required for permeability barrier homeostasis. J Invest Dermatol 1996;106:57-63.
10. 2 in breast carcinoma cells. Oncogene 1995;10:2297-2305.
11. Lunstrom A, Elgerud T: Cell shedding from human plantar skin in vitro: Evidence of its dependence on endogenous proteolysis. J Invest Dermatol 1988;91:340-343.
12. Suzuki Y, Nomura J, Hori J, Takahashi M, Horii I: Detection and characterization of endogenous protease associated with desquamation of stratum corneum. Arch Dermatol Res 1993;285:372-377.
13. Koyama J, Nakanishi J, Masuda Y, Sato J, Nomura J, Suzuki Y, Nakayama Y: The mechanism of desquamation in the stratum corneum and its relevance to skin care. 19th IFSCC Congress, Sydney 1996.
14. Suzuki Y, Koyama J, Moro O, Horii I, Kikuchi K. Tanida M, Tagami H: The role of two endogenous proteases of the stratum corneum in degradation of demoglein-1 and their reduced activity in the skin of ichthyotic patients. Br J Dermatol 1996;134:460-464.
15. Maloney ME, Williams ML, Epstein EH, Law MYZ, Fritsch PO, Elias PM: Lipids in the pathogenesis of ichtyosis: Topical cholesterol sulfate. Induced scaling in hairless mice. J Invest Dermatol 1984;83: 252-256.
16. Iwamori M, Iwamori Y, Ito N: Sulfated lipids as inhibitors of pancreatic trypsin and chymotrypsin in epithelium of the mammalian digestive tract. Biochem Biophys Res Commun 1997;237:262-265.
17. Schloss JV: Significance of slow-binding enzyme inhibition and its relationship to reaction-intermediate analogues. Acc Chem Res 1988;21:348-353.
18. Forestier JP: Les enzymes de l'espace extra-cellulaire du stratum corneum. Int J Cosmet Sci 1992;14:47-63.
19. Redoules D, Tarroux R, Perie JJ: Epidermal enzymes: Their role in homeostasis and their relationships with dermatoses. Skin Pharmacol Appl Skin Physiol 1998;11:183-192.
20. 2. J Biol Chem 1989;264:5768-5775.
21. Kanai K, Kondo E: Substrate response in acid phosphatase activity of Pseudomonas pseudomallei and Pseudomonas cepacia, with special reference to tyrosine phosphatase. Jpn J Med Sci Biol 1991;44:225-237.
22. Stoscheck CM, Friedman DB, King LE: Identification of a phosphotyrosyl-protein phosphatase in mouse epidermis. J Invest Dermatol 1989; 92:379-384.
23. 2. Biochemistry 1993;32:5935-5940.
24. 2 (Naja naja naja). A case of half-site reactivity. J Biol Chem 1977;252:2405-2411.
25. 2 inhibit release of free arachidonate in thrombin-stimulated human platelets. J Biol Chem 1994;269:15625-15630.
26. Elgerud T, Lundstrom A: A chymotrypsin-like proteinase that may be involved in desquamation in plantar stratum corneum. Arch Dermatol Res 1991;283:108-112.
27. van der Molen RG, Spies F, van't Noordende JM, Boelsma E, Mommaas AM, Koerten HK: Tape stripping of human stratum corneum yields cell layers that originate from various depths because of furrows in the skin. Arch Dermatol Res 1997; 289:514-518.
28. Odland GF: Structure of the skin: in Goldsmith LG (ed): Physiology, Biochemistry, and Molecular Biology of the Skin, ed 2. New York, Oxford University Press, 1991, pp 3-62.
29. Zlotogorski A: Distribution of skin surface pH on the forehead and cheek of adults. Arch Dermatol Res 1987;279:398-401.
30. Mauro T, Grayson S, Gao WN, Man MQ, Kriehuber E, Behne M, Feingold K, Elias PM: Barrier recovery is impeded at neutral pH, independent of ionic effects: Implications for extracellular lipid processing. Arch Dermatol Res 1998;290:215-222.
31. Forslind BO: A domain mosaic model of the skin barrier. Acta Derm Venereol (Stockh) 1994;74: 1-6.
32. Finlay AY, Nicholls S, King CS, Marks R: The 'dry' non-eczematous skin associated with atopic eczema. Br J Dermatol 1980;102:249.
33. White MI, Jenkinson DM, Lloyd DH: The effect of washing on the thickness of the stratum corneum in normal and atopic individuals. Br J Dermatol 1987;116:525-530.
34. Prottey C, Olivier D, Coxon C: Prediction and measurement of surfactant action upon human skin under realistic conditions. Int J Cosmet Sci 1984;6:263-273.
35. Jin K, Higaki Y, Takagi Y, Higuchi K, Yada Y, Kawashima M, Imokawa G: Analysis of beta-glucocerebrosidase and ceramidase activities in atopic and aged dry skin. Acta Derm Venereol (Stockh) 1994;74:5337-5340.
36. Folk JE: Transglutaminases. Annu Rev Biochem 1980;49:517-531.
37. Redoules D, Tarroux R, Perie JJ: Pierre Fabre Patent: 9802888, 1998.