Cloning and characterization of a gene, pbpF, encoding a new penicillin- binding protein, PBP2B, in Staphylococcus aureus

Antimicrobial Agents and Chemotherapy

Volumn 43, Issue 7, 1999, Pages 1578-1583

  Komatsuzawa, Hitoshi ^a   Choi, Gil H ^b   Ohta, Kouji ^a   Sugai, Motoyuki ^a   Tran, Monique T ^b   Suginaka, Hidekazu ^a  

^a HIROSHIMA UNIVERSITY   (Japan)

^b GLAXOSMITHKLINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN; DNA; PENICILLIN BINDING PROTEIN; PENICILLIN DERIVATIVE;

AMINO ACID COMPOSITION; AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; BACTERIAL GENE; BINDING AFFINITY; CARBOXY TERMINAL SEQUENCE; DNA SEQUENCE; GENE ISOLATION; MOLECULAR CLONING; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN LOCALIZATION; SEQUENCE ANALYSIS; STAPHYLOCOCCUS AUREUS;

EID: 0032793848     PISSN: 00664804     EISSN: None     Source Type: Journal    
DOI: 10.1128/aac.43.7.1578     Document Type: Article

References (28)

1. Adachi, H., T. Ohta, and H. Matsuzawa. 1987. A water-soluble form of penicillin-binding protein 2 of Escherichia coli constructed by site-directed mutagenesis. FEBS Lett. 226:150-154.
2. Beck, W. D., B. Berger-Bächi, and F. H. Kayser. 1986. Additional DNA in methicillin-resistant Staphylococcus aureus and molecular cloning of mecspecific DNA. J. Bacteriol. 165:373-378.
3. Beise, F., H. Labischinski, and P. Giesbrecht. 1988. Role of the penicillin-binding proteins of Staphylococcus aureus in the induction of bacteriolysis by β-lactam antibiotics, p. 360-366. In P. Actor, L. Daneo-Moore, M. L. Higgins, M. R. J. Salton, and G. D. Shockman (ed.), Antibiotic inhibition of bacterial cell surface assembly and function. American Society for Microbiology, Washington, D.C.
4. Berger-Bächi, B., A. Strässle, and F. H. Kayser. 1989. Natural methicillin resistance in comparison with that selected by in-vitro drug exposure in Staphylococcus aureus. J. Antimicrob. Chemother. 23:179-188.
5. Chambers, H. F., and C. Miick. 1992. Characterization of penicillin-binding protein 2 of Staphylococcus aureus: deacylation reaction and identification of two penicillin-binding peptides. Antimicrob. Agents Chemother. 36:656-661.
6. Curtis, N. A. C., M. V. Hayes, A. W. Wyke, and J. B. Ward. 1980. A mutant of Staphylococcus aureus H lacking penicillin-binding protein 4 and transpeptidase activity. FEMS Microbiol. Lett. 9:263-266.
7. Fleischmann, R. D., M. D. Adams, O. White, R. A. Clayton, E. F. Kirkness, A. R. Kerlavage, C. J. Bult, J. F. Tomb, B. A. Dougherty, J. M. Merrick, et al. 1995. Whole-genome random sequencing and assembly of Haemophilus influenzae Rd. Science 269:496-512.
8. Fontana, R. 1985. Penicillin-binding proteins and the intrinsic resistance to beta-lactams in gram-positive cocci. J. Antimicrob. Chemother. 16:412-416.
9. Georgopapadakou, N. H., and F. Y. Liu. 1980. Binding of β-lactam antibiotics of penicillin-binding proteins of Staphylococcus aureus and Streptococcus faecalis: relation to antibacterial activity. Antimicrob. Agents Chemother. 18:834-836.
10. Goffin, C., and J.-M. Ghuysen. 1998. Multimodular penicillin-binding proteins: an enigmatic family of orthologs and paralogs. Microbiol. Mol. Biol. Rev. 62:1079-1093.
11. Henze, U. U., and B. Berger-Bächi. 1995. Staphylococcus aureus penicillin-binding protein 4 and intrinsic β-lactam resistance. Antimicrob. Agents Chemother. 39:2415-2422.
12. Joris, B., J.-M. Ghuysen, G. Dive, A. Renard, O. Dideberg, P. Charlier, J.-M. Frere, J. A. Kelly, J. C. Boyington, P. C. Moews, and J. R. Knox. 1988. The active-site-serine penicillin-recognizing enzymes as members of the Streptomyces R61 DD-peptidase family. Biochem. J. 250:313-324.
13. Kelly, J. A., J. R. Knox, Z. Haiching, J.-M. Frere, and J.-M. Ghuysen. 1989. Crystallographic mapping of beta-lactams bound to a D-alanyl-D-alanine peptidase target enzyme. J. Mol. Biol. 209:281-295.
14. Komatsuzawa, H., J. Suzuki, M. Sugai, Y. Miyake, and H. Suginaka. 1994. The effect of Triton X-100 on the in-vitro susceptibility of methicillin-resistant Staphylococcus aureus to oxacillin. J. Antimicrob. Chemother. 34:885-897.
15. Kornblum, J., B. J. Hartman, R. P. Novick, and A. Tomasz. 1986. Conversion of a homogeneously methicillin-resistant strain of Staphylococcus aureus to heterogeneous resistance by Tn551-mediated insertional inactivation. Eur. J. Clin. Microbiol. 5:714-718.
16. Moreira, B., S. Boyle-Vavra, B. L. M. de Jonge, and R. S. Daum. 1997. Increased production of penicillin-binding protein 2, increased detection of other penicillin-binding proteins, and decreased coagulase activity associated with glycopeptide resistance in Staphylococcus aureus. Antimicrob. Agents Chemother. 41:1788-1793.
17. Murakami, K., T. Fujimura, and M. Doi. 1994. Nucleotide sequence of the structural gene for the penicillin-binding protein 2 of Staphylococcus aureus and the presence of a homologous gene in other staphylococci. FEMS Microbiol. Lett. 117:131-136.
18. Oefner, C., A. D'Arcy, J. J. Daly, K. Guernator, R. L. Charnas, I. Heinze, C. Hubschwerien, and F. K. Winkler. 1990. Refined crystal structure of beta-lactamase from Citrobacter freundii indicates a mechanism for beta-lactam hydrolysis. Nature (London) 343:284-288.
19. Reynolds, P. E., and C. Fuller. 1986. Methicillin resistant strains of Staphylococcus aureus; presence of an identical additional penicillin-binding protein in all strains examined. FEMS Microbiol. Lett. 33:251-254.
20. Shlaes, D. M., J. H. Shlaes, S. Vincent, L. Etter, P. D. Fey, and R. V. Goering. 1993. Teicoplanin-resistant Staphylococcus aureus expresses a novel membrane protein and increases expression of penicillin-binding protein 2 complex. Antimicrob. Agents Chemother. 37:2432-2437.
21. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
22. Song, M. D., M. Wachi, M. Doi, F. Ishino, and M. Matsuhashi. 1987. Evolution of an inducible penicillin-target protein in methicillin-resistant Staphylococcus aureus by gene fusion. FEBS Lett. 221:167-171.
23. Spratt, B. G., and K. D. Cromie. 1988. Penicillin-binding proteins of Gram-negative bacteria. Rev. Infect. Dis. 10:699-711.
24. Sugai, M., T. Akiyama, H. Komatsuzawa, Y. Miyake, and H. Suginaka. 1990. Characterization of sodium dodecyl sulfate-stable Staphylococcus aureus bacteriolytic enzymes by polyacrylamide gel electrophoresis. J. Bacteriol. 172: 6494-6498.
25. Tomasz, A., H. B. Drugeon, H. M. de Lencastre, D. Jabes, L. McDougall, and J. Bille. 1989. New mechanism for methicillin resistance in Staphylococcus aureus: clinical isolates that lack the PBP 2a gene and contain normal penicillin-binding proteins with modified penicillin-binding capacity. Antimicrob. Agents Chemother. 33:1869-1874.
26. Ubukata, K., R. Nogorochi, M. Matsuhashi, and M. Konno. 1989. Expression and inducibility in Staphylococcus aureus of the mecA gene which encodes a methicillin-resistant S. aureus-specific penicillin-binding protein. J. Bacteriol. 171:2882-2885.
27. Wada, A., and H. Watanabe. 1998. Penicillin-binding protein 1 of Staphylococcus aureus is essential for growth. J. Bacteriol. 180:2759-2765.
28. Wu, C. Y. E., J. Hoskins, L. C. Blaszczak, D. A. Preston, and P. L. Skatrud. 1992. Construction of a water-soluble form of penicillin-binding protein 2a from a methicillin-resistant Staphylococcus aureus isolate. Antimicrob. Agents Chemother. 36:533-539.