Immunological characterization of the sheep prion protein expressed as fusion proteins in Escherichia coli

FEMS Immunology and Medical Microbiology

Volumn 25, Issue 4, 1999, Pages 379-384

  Baron, Thierry G M ^a   Betemps, Dominique ^a   Groschup, Martin H ^b   Madec, Jean Yves ^a  

^a Healthy Pigs Production and Experimentation Section   (France)

^b FEDERAL RESEARCH INSTITUTE FOR ANIMAL HEALTH   (Germany)

Author keywords

Prion; Recombinant protein; Sheep

Indexed keywords

GLUTATHIONE TRANSFERASE; HYBRID PROTEIN; PRION PROTEIN; RECOMBINANT PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; ESCHERICHIA COLI; MOLECULAR CLONING; NONHUMAN; PRION DISEASE; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN EXPRESSION; SHEEP; WESTERN BLOTTING;

ANIMALS; CLONING, MOLECULAR; ESCHERICHIA COLI; GENE EXPRESSION; GLUTATHIONE TRANSFERASE; HISTIDINE; PRIONS; RECOMBINANT FUSION PROTEINS; SHEEP;

EID: 0032792448     PISSN: 09288244     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0928-8244(99)00112-1     Document Type: Article

References (22)

1. Weiss S., Famulok M., Edenhofer F., Wang Y.H., Jones I.M., Groschup M., Winnacker E.L. Overexpression of active syrian golden hamster prion protein PrP C as a glutathione S-transferase fusion in heterologous systems. J. Virol. 69:1995;4776-4783.
2. Mehlhorn I., Groth D., Stockel J., Moffat B., Reilly D., Yansura D., Willett W.S., Baldwin M., Fletterick R., Cohen F.E., Vandlen R., Henner D., Prusiner S.B. High-level expression and characterization of a purified 142-residue polypeptide of the prion protein. Biochemistry. 35:1996;5528-5537.
3. Völkel D., Blankenfeldt W., Schomburg D. Large-scale production, purification and refolding of the full-length cellular prion protein from Syrian golden hamster in Escherichia coli using the glutathione S-transferase-fusion system. Eur. J. Biochem. 251:1998;462-471.
4. Maniatis, T., Fritsch, E.F. and Sambrook, J. (1989) Molecular Cloning: A Laboratory Manual, 2d edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
5. Goldmann W., Hunter N., Foster J.D., Salbaum J.M., Beyreuther K., Hope J. Two alleles of a neural protein gene linked to scrapie in sheep. Proc. Natl. Acad. Sci. USA. 87:1990;2476-2480.
6. Cheynet V., Verrier B., Mallet F. Overexpression of HIV-1 proteins in Escherichia coli by a modified expression vector and their one-step purification. Protein Express. Purif. 4:1993;367-372.
7. Madec J.Y., Vanier A., Dorier A., Bernillon J., Belli P., Baron T. Biochemical properties of protease resistant prion protein PrPsc in natural sheep scrapie. Arch. Virol. 142:1997;1603-1612.
8. Harmeyer S., Pfaff E., Groschup M.H. Synthetic peptide vaccines yield monoclonal antibodies to cellular and pathological prion proteins of ruminants. J. Gen. Virol. 79:1998;937-945.
9. Kascsak R.J., Rubenstein R., Merz P.A., Tonna Demasi M., Fersko R., Carp R.I., Wisniewski H.M., Diringer H. Mouse polyclonal and monoclonal antibody to scrapie-associated fibril proteins. J. Virol. 61:1987;3688-3693.
10. Groschup M.H., Harmeyer S., Pfaff E. Antigenic features of prion proteins of sheep and of other mammalian species. J. Immunol. Methods. 207:1997;89-101.
11. Hornemann S., Korth C., Oesch B., Riek R., Wider G., Wüthrich K., Glockshuber R. Recombinant full-length murine prion protein, mPrP (23-231): purification and spectroscopic characterization. FEBS Lett. 413:1997;277-281.
12. Glockshuber R., Hornemann S., Billeter M., Riek R., Wider G., Wuethrich K. Prion protein structural features indicate possible relations to signal peptidases. FEBS Lett. 426:1998;291-296.
13. Shmerling D., Hegyi I., Fischer M., Blattler T., Brandner S., Gotz J., Rulicke T., Flechsig E., Cozzio A., Von Mering C., Hangartner C., Aguzzi A., Weissmann C. Expression of amino-terminally truncated PrP in the mouse leading to ataxia and specific cerebellar lesions. Cell. 93:1998;203-214.
14. Baron T., Betemps D., Mallet F., Cheynet V., Levy D., Belli P. Detection of bovine immunodeficiency-like virus infection in experimentally infected calves. Arch. Virol. 143:1998;181-189.
15. Negro A., De Filippis V., Skaper D., James P., Sorgato M.C. The complete mature bovine prion protein highly expressed in Escherichia coli: Biochemical and structural studies. FEBS Lett. 412:1997;359-364.
16. Betemps D., Mallet F., Cheynet V., Baron T. Overexpression and purification of an immunologically reactive His-BIV capsid fusion protein. Protein Express. Purif. 15:1999;258-264.
17. Groschup M.H., Pfaff E. Studies on a species-specific epitope in murine, ovine and bovine prion protein. J. Gen. Virol. 74:1993;1451-1456.
18. Bolton D.C., Seligman S.J., Bablanian G., Windsor D., Scala L.J., Kim K.S., Chen C.M.J., Kascsak R.J., Bendheim P.E. Molecular location of a species-specific epitope on the hamster scrapie agent protein. J. Virol. 65:1991;3667-3675.
19. Baron T., Belli P., Madec J.Y., Moutou F., Vitaud C., Savey M. Spongiform encephalopathy in an imported cheetah in France. Vet. Rec. 141:1997;270-271.
20. Kascsak R.J., Fersko R., Pulgiano D., Rubenstein R., Carp R.I. Immunodiagnosis of prion disease. Immunol. Invest. 26:1997;259-268.
21. Collinge J., Sidle K.C.L., Meads J., Ironside J., Hill A.F. Molecular analysis of prion strain variation and the aetiology of 'new variant' CJD. Nature. 383:1996;685-690.
22. Rogers M., Serban D., Gyuris T., Scott M., Torchia T., Prusiner S.B. Epitope mapping of the syrian hamster prion protein utilizing chimeric and mutant genes in a vaccinia virus expression system. J. Immunol. 147:1991;3568-3574.