An RGS protein regulates the pheromone response in the fission yeast Schizosaccharomyces pombe

Molecular Microbiology

Volumn 33, Issue 3, 1999, Pages 623-634

  Watson, Peter ^a   Davis, Kevin ^a   Didmon, Mark ^a   Broad, Peter ^b   Davey, John ^a  

^a UNIVERSITY OF WARWICK   (United Kingdom)

^b ASTRAZENECA   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

GUANINE NUCLEOTIDE BINDING PROTEIN; PHEROMONE; REGULATOR PROTEIN;

ARTICLE; CELL STIMULATION; NONHUMAN; PRIORITY JOURNAL; SCHIZOSACCHAROMYCES POMBE; SIGNAL TRANSDUCTION; YEAST;

AMINO ACID SEQUENCE; FUNGAL PROTEINS; GENE EXPRESSION REGULATION, FUNGAL; GENES, FUNGAL; GENES, REPORTER; GTPASE-ACTIVATING PROTEINS; MOLECULAR SEQUENCE DATA; PHEROMONES; PROTEINS; RGS PROTEINS; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; SCHIZOSACCHAROMYCES; SCHIZOSACCHAROMYCES POMBE PROTEINS; SEQUENCE ANALYSIS, DNA; SEQUENCE HOMOLOGY, AMINO ACID; SIGNAL TRANSDUCTION; TRANSCRIPTION, GENETIC;

FUNGI; MYXOGASTRIA; SACCHAROMYCES CEREVISIAE; SACCHAROMYCETALES; SCHIZOSACCHAROMYCES POMBE;

EID: 0032792215     PISSN: 0950382X     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2958.1999.01510.x     Document Type: Article

References (53)

1. Aono, T., Yanai, H., Miki, F., Davey, J., and Shimoda, C. (1994) Mating pheromone-induced expression of the mat1-Pm gene of Schizosaccharomyces pombe: identification of signalling components and characterisation of upstream controlling elements. Yeast 10: 757-770.
2. Basi, G., Schmid, E., and Maundrell, K. (1993) TATA box mutations in the Schizosaccharomyces pombe nmt1 promoter affect transcription efficiency but not the transcription start point or thiamine repressibility. Gene 123: 131-136.
3. Berman, D.M., and Gilman, A.G. (1998) Mammalian RGS proteins: barbarians at the gate. J Biol Chem 273: 1269-1272.
4. Berman, D.M., Wilki, T.M., and Gilman, A.G. (1996) GAIP and RGS4 are GTPase-activating proteins for the Gi subfamily of G protein α-subunits. Cell 86: 445-452.
5. Boeke, J.D., Trueheart, J., Natsoulis, G., and Fink, G.R. (1987) 5′-fluroorotic acid as a selective agent in yeast molecular genetics. Methods Enzymol 152: 164-175.
6. Böhm, S.K., Grady, E.F., and Bunnett, N.W. (1997) Regulatory mechanisms that modulate signalling by G protein-coupled receptors. Biochem J 322: 1-18.
7. Chan, R.K., and Otte, C.A. (1982) Physiological characterization of Saccharomyces cerevisiae mutants supersensitive to G1 arrest by a-factor and α-factor pheromones. Mol Cell Biol 2: 21-29.
8. Chen, C., Zheng, B., Han, J., and Lin, S.-C. (1997) Characterization of a novel mammalian RGS protein that binds to Gα proteins and inhibits pheromone signaling in yeast. J Biol Chem 272: 8679-8685.
9. Davey, J. (1992) Mating pheromones of the fission yeast Schizosaccharomyces pombe: purification and structural characterisation of M-factor and isolation and structural characterisation of two genes encoding the pheromone. EMBO J 11: 951-960.
10. Davey, J. (1998) Fusion of a fission yeast. Yeast 14: 1529-1566.
11. Davey, J., and Nielsen, O. (1994) Mutations in cyr1 and pat1 reveal pheromone-induced G1 arrest in the fission yeast Schizosaccharomyces pombe. Curr Genet 26: 105-112.
12. Davey, J., Egel, R., and Nielsen, O. (1995) Pheromone procedures in fission yeast. In Methods in Molecular Genetics, Vol. 6. Adolph, K.W. (ed.), San Diego: Academic Press, pp. 247-263.
13. De Vries, L., Elenko, E., Huber, L., Jones, T.L.Z., and Farquhar, M.G. (1996) GAIP is membrane-anchored by palmitoylation and interacts with the activated (GTP-bound) form of Gαi subunits. Proc Natl Acad Sci USA 93: 15203-15208.
14. De Vries, L., Lou, X., Zhao, G., Zheng, B., and Farquhar, M.G. (1998) GIPC, a PDZ domain containing protein, interacts specifically with the C-terminus of RGS-GAIP. Proc Natl Acad Sci USA 95: 12340-12345.
15. Dohlman, H.G., and Thorner, J. (1997) RGS proteins and signaling by heterotrimeric G proteins. J Biol Chem 272: 3871-3874.
16. Dohlman, H.G., Apaniesk, D., Chen, Y., Song, J., and Nusskern, D. (1995) Inhibition of G protein signalling by dominant gain-of-function mutations in Sst2p, a pheromone desensitisation factor in Saccharomyces cerevisiae. Mol Cell Biol 15: 3635-3643.
17. Dohlman, H.G., Song, J.P., Ma, D.R., Courchesne, W.E., and Thorner, J. (1996) SST2, a negative regulator of pheromone signalling in the yeast Saccharomyces cerevisiae: expression, localisation and genetic interaction and physical association with GPA1 (the G protein α subunit). Mol Cell Biol 16: 5194-5209.
18. Druey, K.M., Blumer, K.J., Kang, V.H., and Kehrl, J.H. (1996) Inhibition of G protein-mediated MAP kinase activation by members of a novel mammalian gene family. Nature 379: 742-746.
19. Egel, R. (1992) Pheromone detection and pheromone hyper-sensitive mutants in the fission yeast Schizosaccharomyces pombe. Yeast 8: S363.
20. Egel, R., Willer, M., Kjaerulff, S., Davey, J., and Nielsen, O. (1994) Assessment of pheromone production and response in fission yeast by a halo test of induced sporulation. Yeast 10: 1347-1354.
21. Faurobert, E., and Hurley, J.B. (1997) The core domain of a new retina specific RGS protein stimulates the GTPase activity of transducin in vitro. Proc Natl Acad Sci USA 94: 2945-2950.
22. Freedman, N.J., and Lefkowitz, R.J. (1996) Desensitisation of G protein-coupled receptors. Recent Prog Hormone Res 51: 319-353.
23. Fukui, Y., Kozasa, T., Kaziro, Y., Takeda, T., and Yamamoto, M. (1986) Role of a ras homolog in the life cycle of Schizosaccharomyces pombe. Cell 44: 329-336.
24. Grimm, C., Kohli, J., Murray, J., and Maundrell, K. (1988) Genetic engineering of Schizosaccharomyces pombe: a system for gene disruption and replacement using the ura4 gene as a selectable marker. Mol Gen Genet 215: 81-86.
25. Guarente, L. (1983) Yeast promoters and lacZ fusions designed to study expression of cloned genes in yeast. Methods Enzymol 101: 181-191.
26. Hepler, J.R., Berman, D.M., Gilman, A.G., and Kozasa, T. (1997) RGS4 and GAIP are GTPase-activating proteins for Gαq and block activation of phospholipase C-β by γ-GTP-Gαq. Proc Natl Acad Sci USA 94: 428-432.
27. Huang, C., Hepler, J.R., Gilman, A.G., and Mumby, S.M. (1997) Attenuation of Gi-and Gq-mediated signaling by expression of RGS4 or GAIP in mammalian cells. Proc Natl Acad Sci USA 94: 6159-6163.
28. Imai, Y., and Yamamoto, M. (1992) Schizosaccharomyces pombe sxa1+ and sxa2+ encode putative proteases involved in the mating response. Mol Cell Biol 12: 1827-1834.
29. Imai, Y., and Yamamoto, M. (1994) The fission yeast mating pheromone P-factor: its molecular structure, gene structure and physiological activities to induce gene expression and G1 arrest in the mating partner. Genes Dev 8: 328-338.
30. Itoh, K., Krupnik, V.E., and Sokol, S.Y. (1998) Axis determination in Xenopus involves biochemical interactions of axin, glycogen synthase kinase-3 and β-catenin. Curr Biol 8: 591-594.
31. Kelly, M., Burke, J., Smith, M., Klar, A., and Beach, D. (1988) Four mating-type genes control sexual differentiation in the fission yeast. EMBO J 7: 1537-1547.
32. Kjaerulff, S., Dooijes, D., Clevers, H., and Nielsen, O. (1997) Cell differentiation by interaction of two HMG-box proteins: Mat1-Mc activates M cell-specific genes in S. pombe by recruiting the ubiquitous transcription factor Ste11 to weak binding sites. EMBO J 16: 4021-4033.
33. Klar, A.J.S., and Miglio, L.M. (1986) Initiation of meiotic recombination by double-strand DNA breaks in S. pombe. Cell 46: 725-731.
34. Koelle, M.R., and Horvitz, H.R. (1996) EGL-10 regulates G protein signalling in the C. elegans nervous system and shares a conserved domain with many mammalian proteins. Cell 84: 115-125.
35. Ladds, G., Rasmussen, E.M., Young, T., Nielsen, O., and Davey, J. (1996) The sxa2-dependent inactivation of the P-factor mating pheromone in the fission yeast Schizosaccharomyces pombe. Mol Microbiol 20: 35-42.
36. Lee, B.N., and Adams, T.H. (1994) Overexpression of FlbA, an early regulator of Aspergillus asexual sporulation, leads to activation of BrlA and premature initiation of development. Mol Microbiol 14: 323-334.
37. Maundrell, K. (1990) nmt1 of fission yeast: a highly transcribed gene completely repressed by thiamine. J Biol Chem 265: 10857-10864.
38. Maundrell, K. (1993) Thiamine-repressible expression vectors pREP and pRIP for fission yeast. Gene 123: 127-130.
39. Miyajima, I., Nakafuku, M., Nakayama, N., Brenner, C., Miyajima, A., Kaibuchi, K. et al. (1987) GPA1, a haploid-specific essential gene, encodes a yeast homolog of mammalian G protein which may be involved in mating factor signal transduction. Cell 50: 1011-1019.
40. Nakafuku, M., Itoh, H., Nakamura, S., and Kaziro, Y. (1987) Occurrence in Saccharomyces cerevisiae of a gene homologous to the cDNA coding for the α-subunit of mammalian G proteins. Proc Natl Acad Sci USA 84: 2140-2144.
41. Nielsen, O., Davey, J., and Egel, R. (1992) The ras1 function of Schizosaccharomyces pombe mediates pheromone-induced transcription. EMBO J 11: 1391-1395.
42. Obara, T., Nakafuku, M., Yamamoto, M., and Kaziro, Y. (1991) Isolation and characterisation of a gene encoding a G-protein α-subunit from Schizosaccharomyces pombe: involvement in mating and sporulation pathways. Proc Natl Acad Sci USA 88: 5877-5881.
43. Popov, S., Yu, K., Kozasa, T., and Wilkie, T.M. (1997) The regulators of G protein signalling (RGS) domains of RGS4, RGS10, and GAIP retain GTPase activating protein activity in vitro. Proc Natl Acad Sci USA 94: 7216-7220.
44. Siderovski, D.P., Heximer, S.P., and Forsdyke, D.R. (1994) A human gene encoding a putative basic helix-loop-helix phosphoprotein whose mRNA increases rapidly in cyctoheximide-treated blood mononuclear cells. DNA Cell Biol 13: 125-147.
45. Snow, B.E., Hall, R.A., Krumins, A.M., Brothers, G.M., Bouchard, D., Brothers, C.A., et al. (1998a) GTPase activating specificity of RGS12 and binding specificity of an alternatively spliced PDZ (PSD-95/Dlg/ZO-1) domain. J Biol Chem 273: 17749-17755.
46. Snow, B.E., Krumins, A.M., Brothers, G.M., Lee, S.-F., Wall, M.A., Chung, S. et al. (1998b) A G protein γ subunit-like domain shared between RGS11 and other RGS proteins specifies binding to Gβ5 subunits. Proc Natl Acad Sci USA 95: 13307-13312.
47. Srinivasa, S.P., Bernstein, L.S., Blumer, K.J., and Linder, M.E. (1998) Plasma membrane localization is required for RGS4 function in Saccharomyces cerevisiae. Proc Natl Acad Sci USA 95: 5584-5589.
48. Tesmer, J.J.G., Berman, D.M., Gilman, A.G., and Sprang, S.R. (1997) Structure of RGS4 bound to AIF (4-) -activated Gαi1: stabilisation of the transition state for GTP hydrolysis. Cell 89: 251-261.
49. Watson, P., and Davey, J. (1998) Characterization of the Prk1 protein kinase from Schizosaccharomyces pombe. Yeast 14: 485-492.
50. Yamawaki-Kataoka, Y., Tamaoki, T., Choe, H.-R., Tanaka, H., and Kataoka, T. (1989) Adenylate cyclases in yeast: a comparison of the genes from Schizosaccharomyces pombe and Saccharomyces cerevisiae. Proc Natl Acad Sci USA 86: 5693-5697.
51. Young, D., Riggs, M., Field, J., Vojtek, A., Broek, D., and Wigler, M. (1989) The adenylyl cyclase gene from Schizosaccharomyces pombe. Proc Natl Acad Sci USA 86: 7989-7993.
52. Yun, D.-F., Laz, T.M., Clements, J.M., and Sherman, F. (1996) mRNA sequences influencing translation and the selection of AUG initiator codons in the yeast Saccharomyces Cerevisiae. Mol Microbiol 19: 1225-1239.
53. Zerangue, N., and Jan, L.Y. (1998) G protein signaling: fine-tuning signaling kinetics. Curr Biol 8: R313-R316.