Human sweat gland myoepithelial cells express a unique set of cytokeratins and reveal the potential for alternative epithelial and mesenchymal differentiation states in culture

Journal of Cell Science

Volumn 112, Issue 12, 1999, Pages 1925-1936

  Schön, Margarete ^a,c   Benwood, Jennifer ^a   O'Connell Willstaedt, Therese ^b   Rheinwald, James G ^a,b  

^a BRIGHAM AND WOMEN S HOSPITAL   (United States)

^b HARVARD MEDICAL SCHOOL   (United States)

^c HEINRICH HEINE UNIVERSITY   (Germany)

Author keywords

Actin; Apocrine; Cultured cell; Eccrine; Keratin

Indexed keywords

ALPHA ACTIN; CYTOKERATIN; EPIDERMAL GROWTH FACTOR; HYDROCORTISONE; KERATIN; UVOMORULIN; VIMENTIN;

ARTICLE; CELL CULTURE; CELL DIFFERENTIATION; CELL DIVISION; EPITHELIOID CELL; HUMAN; HUMAN CELL; IMMUNOBLOTTING; IMMUNOCYTOCHEMISTRY; IMMUNOHISTOCHEMISTRY; KERATINOCYTE; MESOTHELIUM CELL; MYOEPITHELIUM CELL; PRIORITY JOURNAL; SERUM; SKIN; SPINDLE CELL; SWEAT GLAND; WESTERN BLOTTING;

BIOPSY; CELL DIFFERENTIATION; CELLS, CULTURED; EPITHELIAL CELLS; HUMANS; IMMUNOHISTOCHEMISTRY; KERATINOCYTES; KERATINS; MESODERM; ORGAN CULTURE TECHNIQUES; SKIN; SWEAT GLANDS;

EID: 0032789942     PISSN: 00219533     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (52)

1. Adams, J. C. and Watt, F. M. (1988). An unusual strain of human keratinocytes which do not stratify or undergo terminal differentiation in culture. J. Cell Biol. 107, 1927-1938.
2. Allen-Hoffmann, B. L. and Rheinwald, J. G. (1984). Polycyclic aromatic hydrocarbon mutagenesis of human epidermal keratinocytes in culture. Proc: Nat. Acad. Sci. USA 81, 7802-7806.
3. Bartek, J., Vojtesek, B., Staskova, Z., Bartkova, J., Kerekes, Z., Rejthat, A. and Kovarik, J. (1991). A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology. J. Pathol. 164, 215-224.
4. Bell, C. L. and Quinton, P. M. (1991). Effects of media buffer systems on growth and electrophysiologic characteristics of cultured sweat duct cells. In Vitro Cell Dev. Biol. 27, 47-54.
5. Caselitz, J., Walther, B., Wustrow, J., Seifert, G., Weber, K. and Osborn, M. (1986). A monoclonal antibody that detects myoepithelial cells in exocrine glands, basal cells in other epithelia and basal and suprabasal cells in certain hyperplastic tissues. Virchows Arch A Pathol. Anal. Histopathol. 409, 725-738.
6. 7 integrin. Nature 372, 190-193.
7. Cohn, B. A. (1994). In search of human skin pheromones. Arch Dermatol. 130, 1048-1051.
8. Collie, G., Buchwald, M., Harper, P. and Riordan, J. R. (1985). Culture of sweat gland epithelial cells from normal individuals and patients with cystic fibrosis. In Vitro Cell Dev Biol. 21, 597-602.
9. Connell, N. D. and Rheinwald, J. G. (1983). Regulation of the cytoskeleton in mesothelial cells: reversible loss of keratin and increase in vimentin during rapid growth in culture. Cell 34, 245-253.
10. Debus, E., Weber, K. and Osborn, M. (1982). Monoclonal cytokeratin antibodies that distinguish simple from stratified squamous epithelia: Characterisation on human tissues. EMBO J. 1, 1641-1647.
11. Fischer, H. P., Altmannsberger, M., Weber, K. and Osborn, M. (1987). Keratin polypeptides in malignant epithelial liver tumors. Differential diagnostic and histogenic aspects. Am. J. Pathol. 127, 530-537.
12. Franke, W., Schmid, E., Reudenstein, C., Appelhans, B., Osborn, M., Weber, K. and Keenan, T. W. (1980). Intermediate-sized filaments of prekeratin type in myoepithelial cells. J. Cell Biol. 84, 633-654.
13. Franke, W. W., Schiller, D. L., Hatzfield, M. and Winter, S. (1983). Protein complexes of intermediate-sized filaments: melting of cytokeratin complexes in urea reveals different polypeptide separation characteristics. Proc. Nat. Acad. Sci. USA 80, 7113-7117.
14. Gomm, J. J., Browne, P. J., Coope, R. C., Liu, Q. Y., Buluwela, L. and Coombes, R. C. (1995). Isolation of pure populations of epithelial and myoepithelial cells from the normal human mammary gland using immunomagnetic separation with dynabeads. Anal. Biochem. 226, 91-99.
15. Holbrook, K. A. and Wolff, K. (1993). The structure and development of skin. In Dermatology in General Medicine (ed. T. B. Fitzpatrick, A. Z. Eisen, K. Wolff, I. M. Freedberg and K. F. Austen), pp. 97-145. McGraw-Hill, New York.
16. Jones, C. J., Bell, C. L. and Quinton, P. M. (1988). Different physiological signatures of sweat gland secretory and duct cells in culture. Am. J. Physiol. 255, C102-C111.
17. Rarsten, U., Papsdorf, G., Roloff, G., Stolley, P., Abel, H., Walther, I. and Weiss, H. (1985). Monoclonal anti-cytokeratin antibody from a hybridoma clone generated by electrofusion. Eur. J. Cancer Clin. Oncol. 21, 733-740.
18. LaRocca, P. J. and Rheinwald, J. G. (1984). Coexpression of simple epithelial keratins and vimentin by human mesothelium and mesothelioma in vivo and in culture. Cancer Res. 44, 2991-2999.
19. Lee, C. M., Carpenter, F., Coaker, T. and Kealey, T. (1986). The primary culture of epithelia from the secretory coil and collecting duct of normal human and cystic fibrotic eccrine sweat glands. J. Cell Sci. 83, 103-118.
20. Leigh, I. M., McKay, I., Carver, N., Navsaria, H. and Green, C. (1991). Skin equivalents and cultured skin: from the petri dish to the patient. Wounds 3, 141-148.
21. Lemullois, M., Rossignol, B. and Mauduit, P. (1996). Immunolocalization of myoepithelial cells in isolated acini of rat exorbital lacrimal gland: cellular distribution of muscarinic receptors. Biol. Cell 86, 175-181.
22. Lynch, M. H., O'Guin, W. M., Hardy, C., Mak, L. and Sun, T.-T. (1986). Acidic and basic hair/nail ('hard') keratins: their co-localization in upper cortical and cuticle cells of the human hair follicle and their relationship to 'soff keratins. J. Cell Biol. 181, 2593-2606.
23. Martin, P. (1997). Wound healing - aiming for perfect skin regeneration. Science 276, 75-81.
24. Moll, I. and Moll, R. (1992). Changes of expression of intermediate filament proteins during ontogenesis of eccrine sweat glands. J. Invest. Dermatol. 98, 777-785.
25. Moll, R., Franke, W. W. and Schiller, D. L. (1982). The catalog of human cytokeratins: Patterns of expression in normal epithelia, tumors and cultured cells. Cell 31, 11-24.
26. Moll, R., Krepler, R. and Franke, W. W. (1983). Complex cytokeratin polypeptide patterns observed in certain human carcinomas. Differentiation 23, 256-269.
27. Morimoto, Y. and Saga, K. (1995). Proliferating cells in human eccrine and apocrine sweat glands. J. Histochem. Cytochem. 43, 1217-1221.
28. Norberg, L., Dardick, I., Leung, R., Burford-Mason, A. and Rippstein, P. (1992). Immunogold localization of actin and cytokeratin filaments in myoepithelium of human parotid salivary gland. Ultrastruct Pathol. 16, 555-568.
29. O'Connor, N. E., Mulliken, J. B., Banks-Schlegel, S., Kehinde, O. and Green, H. (1981). Grafting of burns with cultured epithelium prepared from autologous epidermal cells. The Lancet 10, 75-78.
30. O'Hare, M. J., Ormerod, M. G., Monaghan, P., Lane, E. B. and Gusterson, B. A. (1991). Characterization in vitro of luminal and myoepithelial cells isolated from the human mammary gland by cell sorting. Differentiation 46, 209-221.
31. Osborn, M., Debus, E. and Weber, K. (1984). Monoclonal antibodies specific for vimentin. Eur. J. Cell Biol. 34, 137-143.
32. Parenteau, N. L., Nolte, C. M., Bilbo, P., Rosenberg, M., Wilkins, L. M., Johnson, E. W., Watson, S., Mason, V. S. and Bell, E. (1991). Epidermis generated in vitro: Practical considerations and applications. J. Cell. Biochem. 45, 245-251.
33. Pedersen, P. S. (1989). Human sweat duct cells in primary culture. Basic bioelectric properties of cultures derived from normals and patients with cystic fibrosis. In Vitro Cell Dev. Biol. 25, 342-352.
34. Peterson, O. W. and van Deurs, B. (1988). Growth factor control of myoepithelial-cell differentiation in cultures of human mammary gland. Differentiation 39, 197-215.
35. Pirisi, L., Yasumoto, S., Feller, M., Doniger, J. and DiPaolo, J. A. (1987). Transformation of human fibroblasts and keratinocytes with human papillomavirus type 16 DNA. J. Virol. 61, 1061-1066.
36. Ramaekers, F., Hujsmans, A., Moesker, O., Kant, A., Jap, P., Herman, C. and Vooijs, P. (1983). Monoclonal antibody to keratin filaments, specific for glandular epithelia and their tumors. Lab. Invest. 49, 353-361.
37. Rheinwald, J. G. and Green, H. (1975). Serial cultivation of strains of human epidermal keratinocytes: The formation of keratinizing colonies from single cells. Cell 6, 331-344.
38. Rheinwald, J. G. and Beckett, M. A. (1980). Defective terminal differentiation in culture as a consistent and selectable character of malignant human keratinocytes. Cell 22, 629-632.
39. Rheinwald, J. G. and O'Connell, T. M. (1985). Intermediate filament proteins as distinguishing markers of cell type and differentiated state in cultured human urinary tract epithelia. Ann. NY Acad. Sci. 455, 259-267.
40. Rheinwald, J. G. (1989). Methods for clonal growth and serial cultivation of normal human epidermal keratinocytes and mesothelial cells. In Cell Growth and Division: A Practical Approach (ed. R. Baserga), pp. 81-94. IRL Press, Oxford.
41. Rollins, B. J., O'Connell, T. M., Bennett, G., Burton, L. E., Stiles, C. D. and Rheinwald, J. G. (1989). Environment-dependent growth inhibition of human epidermal keratinocytes by recombinant human transforming growth factor-β. J. Cell. Physiol. 139, 455-462.
42. Saga, K. and Takahashi, M. (1992). Immunoelectron microscopic localization of epidermal growth factor in eccrine and apocrine sweat glands. J. Histochem. Cytochem. 40, 241-249.
43. Sato, K., Nishiyama, A. and Kobayashi, M. (1979). Mechanical properties and functions of the myoepithelium in the eccrine sweat gland. Am. J. Physiol. 237, C177-C184.
44. Sato, K. (1993). Biology of the eccrine sweat gland. In Dermatology in General Medicine (ed. T. B. Fitzpatrick, A. Z. Eisen, K. Wolff, I. M. Freedberg and K. F. Austen), pp. 221-241. McGraw-Hill, New York.
45. Schön, M. and Rheinwald, J. G. (1996). A limited role for retinoic acid and retinoic acid receptors RARα and RARβ in regulating keratin 19 expression and keratinization in oral and epidermal keratinocytes. J. Invest. Dermatol. 107, 428-438.
46. Skalli, O., Ropraz, P., Treciak, A., Benzonana, G., Gillessen, D. and Gabbiani, G. (1986). A monoclonal antibody against α-smooth muscle actin: a new probe for smooth muscle differentiation. J. Cell Biol. 103, 2787-2796.
47. Southgate, J., Williams, H. K., Trejdosiewicz, L. K. and Hodges, G. M. (1987). Primary culture of human oral epithelial cells. Growth requirements and expression of differentiated characteristics. Lab. Invest. 56, 211-223.
48. Tölle, H.-G., Weber, K. and Osborn, M. (1985). Microinjection of monoclonal antibodies specific for one intermediate filament protein in cells containing multiple keratins allow insight into the composition of particular 10 nm filaments. Eur. J. Cell Biol. 38, 234-244.
49. Troyanovsky, S. M., Guelstein, V. I., Tchipysheva, T. A., Krutovskikh, V. A. and Bannikov, G. A. (1989). Patterns of expression of keratin 17 in human epithelia: dependency on cell position. J. Cell Sci. 93, 419-426.
50. van Niekerk, C. C., Jap, P. H., Ramaekers, F. C., van de Molengraft, F. and Poels, L. G. (1991). Immunohistochemical demonstration of keratin 7 in routinely fixed paraffin-embedded human tissues. J. Pathol. 165, 145-152.
51. Wu, Y.-J., Parker, L. M., Binder, N. E., Beckett, M. A., Sinard, J. H., Griffith, C. T. and Rheinwald, J. G. (1982). The mesothelial keratins: A new family of cytoskeletal proteins identified in cultured mesothelial cells and nonkeratinizing epithelia. Cell 31, 693-703.
52. Yokozeki, H., Saga, K., Sato, F. and Sato, K. (1990). Pharmacological responsiveness of dissociated native and cultured eccrine secretory coil cells. Am. J. Physiol. 258 (Reg Int Comp Physiol 27), R1355-R1362.