Use of a mechanical dissociation device to improve standardization of flow cytometric cytokeratin DNA measurements of colon carcinomas

Communications in Clinical Cytometry

Volumn 38, Issue 4, 1999, Pages 184-191

  Brockhoff, Gero ^a   Fleischmann, Sonja ^a   Meier, Anke ^a   Wachs, Frank Peter ^a   Hofstaedter, Ferdinand ^a   Knuechel, Ruth ^a  

^a UNIVERSITY OF REGENSBURG   (Germany)

Author keywords

DNA, cytokeratin; Dual parameter staining; Flow cytometry; Ploidy; S phase; Standardization

Indexed keywords

CYTOKERATIN; DNA; FLUORESCEIN ISOTHIOCYANATE; PROPIDIUM IODIDE;

ARTICLE; CELL CYCLE S PHASE; CELL ISOLATION; COLON CARCINOMA; CONTROLLED STUDY; DEVICE; DNA DETERMINATION; FLOW CYTOMETRY; HUMAN; HUMAN TISSUE; IMMUNOFLUORESCENCE; MAJOR CLINICAL STUDY; PLOIDY; PRIORITY JOURNAL; STAINING; STANDARDIZATION; TISSUE PREPARATION;

CARCINOMA; COLONIC NEOPLASMS; DNA, NEOPLASM; FLOW CYTOMETRY; HUMANS; KERATINS; PROGNOSIS; STAINING AND LABELING;

PHOENIX;

EID: 0032787816     PISSN: 01964763     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0320(19990815)38:4<184::AID-CYTO5>3.0.CO;2-W     Document Type: Article

References (45)

1. Bauer KD, Lincoln ST, Vera-Roman JM, Wallemark CB, Chmiel JS, Madurski ML, Murad T, Scarpelli D. Prognostic implications of proliferative activity and DNA aneuploidy in colonic adenocarcinomas. Lab Invest 1987;57:329-335.
2. Bauer KD, Bagwell CB, Giaretti W, Melamed M, Zarbo RJ, Witzig TE, Rabinovitch PS. Consensus review of the clinical utility of DNA flow cytometry in colorectal cancer. Cytometry 1993;14:486-491.
3. Bauer KD. Colorectal neoplasia. In: Bauer KD, Duque RE, Shankey TV, editors. Clinical flow cytometry-"principals and applications. Baltimore: Williams & Wilkins; 1992. p 307-317.
4. Bergers E, Montironi R, van Diest PJ, Prete E, Baak JPA. Interlaboratory reproducibility of semiautomated cell cycle analysis of flow cytometric DNA histograms obtained from fresh material of 1295 breast cancer cases. Hum Pathol 1996;27:553-560.
5. Bergers E, van Diest PJ, Baak JPA. Cell cycle analysis of 932 flow cytometric DNA histograms of fresh frozen breast carcinoma material. Cancer 1996;77:2258-2266.
6. Bergers E, van Diest PJ, Baak JPA. Tumour heterogeneity of DNA cell cycle variables in breast cancer measured by flow cytometry. J Clin Pathol 1996;49:931-937.
7. Bergers E, van Diest PJ, Baak JPA. Comparison of five cell cycle analysis models applied to 1,414 flow cytometric DNA histograms of fresh frozen breast cancer. Cytometry 1997;30:54-60.
8. Bergers E, Baak JPA, van Diest PJ, van Gorp LHM, Kwee WS, Los J, Peterse HL, Ruitenberg HM, Schapers RFM, Somsen JG, van Beek MWPM, Bellot SM, Fijnheer J. Prognostic implications of different cell cycle analysis models of flow cytometric DNA histograms of 1301 breast cancer patients: results from the multicenter morphometric mammary carcinoma project (MMMPC). Int J Cancer 1997;74:260-269.
9. Bergers E, Jannink I, van Diest PI, Cuesta MA, Meyer S, van Mourik JC, Baak JPA. The influence of fixation delay on mitotic activity and flow cytometric cell cycle variables. Hum Pathol 1997;28:95-100.
10. Bergstrom C, Emdin S, Roos G, Stenling R. DNA content in colorectal carcinoma: a flow cytometric study of the epithelial fraction. Anal Cell Pathol 1995;8:287-295.
11. Bocker T, Schlegel J, Kullmann F, Stumm G, Zirngibl H, Epplen JT, Rueschoff J. Genomic instability in colorectal carcinomas: comparison of different evaluation methods and their biological significance. J Pathol 1996;179:15-19.
12. Brockhoff G, Hofstaedter F, Knuechel R. Flow cytometric detection and quantitation of the epidermal growth factor receptor in comparison to Scatchard analysis in human bladder carcinoma cell lines. Cytometry 1994;17:75-83.
13. Brockhoff G, Endl E, Minuth W, Hofstaedter F, Knuechel R. Options of flow cytometric three-color DNA measurements using single laser instruments to quantitate EGFR in subpopulations of human bladder cancer. Anal Cell Pathol 1996;11:55-70.
14. Brockhoff G, Wieland W, Hofstaedter F, Knuechel R. Evaluation of flow cytometric three-parameter DNA staining to quantify EGFR content in human bladder carcinomas. Virch Arch 1998;432:77-84.
15. Brown RD, Linden MD, Mackowiak P, Kubus JJ, Zarbo RJ, Rabinovitch PS. The effect of number of histogram events on reproducibility and variation of flow cytometric proliferation measurement. Am J Clin Pathol 1996;105:696-704.
16. Chistyakova OV, Zubrikhina GN. Flow cytometric analysis of DNA content in gastric and colorectal cancer. Anal Quant Cytol Histol 19998;20:52-58.
17. Corver WE, Cornelisse CJ, Hermans-J, Fleuren GJ. Limited loss of nine tumor-associated surface antigenic determinants after tryptic cell dissociation. Cytometry 1995;19:267-272.
18. Dietmaier W, Wallinger S, Bocker T, Kullmann F, Fishel R, Rueschhoff J. Diagnostic microsatellite instability: definition and correlation with mismatch repair protein expression Cancer Res 1997;57:4749-4756.
19. Glogovac JK, Porter PL, Banker DE, Rabinovitch PS. Cytokeratin labeling of breast cancer cells extracted from paraffin-embedded tissue for bivariate flow cytometric analysis. Cytometry 1996;24:260-267.
20. Hedley DW, Friedlander ML, Taylor IW, Rugg CA, Musgrove EA. Method for analysis of cellular DNA content of paraffin embedded pathological material using flow cytometry. J Histochem Cytochem 1983;31:1333-1355.
21. Hitchcock CL, Ensley JF. Technical consideration for dissociation of fresh and archival tumors. In: Bauer KD, Duque RE, Shankey TV, editors. Clinical flow cytometry - principals and applications Baltimore: Williams & Wilkins; 1992. p 93-109.
22. Kal HB, van Berkel AH, Broers JL, Klein JC, Mijnheere EP, Roholl PJ, Zurcher C, Ramaekers FC. Cytokeratins expressed in experimental rat bronchial carcinomas. Int J Cancer 1993;53:506-513.
23. Lanza G, Gafa R, Santini A, Maestri I, Dubini A, Gilli G, Cavazzini L. Prognostic significance of DNA ploidy in patients with stage II and stage III colon carcinoma: a prospective flow cytometric study. Cancer 1998;82:49-59.
24. Leers MPG, Theunissen PHMH, Schutte B, Ramaekers FCS. Bivariate cytokeratin/DNA flow cytometric analysis of paraffin-embedded samples from colorectal carcinomas. Cytometry 1995;21:101-107.
25. Leers MPG, Theunissen PHMH, Koudstaal H, Schutte B, Ramaekers FCS. Trivariate flow cytometric analysis of paraffin-embedded lung cancer specimens: application of cytokeratin subtype specific antibodies to distinguish between differentiation pathways. Cytometry 1997; 27:179-188.
26. Moll R, Franke WW, Schiller DL, Geiger B, Krepler R. The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells. Cell 1982;31:11-24.
27. Nylander K, Stenling R, Gustafsson H, Roos G. Application of dual parameter analysis in flow cytometric DNA measurements of paraffin-embedded samples. J Oral Pathol Med 1994;23:190-192.
28. Ottesen GL, Christensen IJ, Larsen JK, Hansen B, Andersen JA. Tissue disaggregation for flow cytometric DNA analysis: comparison of fine-needle aspiration and an automated mechanical procedure. Cytometry 1996;26:65-68.
29. Rabinovitch PS. Practical considerations for DNA content and cell cycle analysis. In: Bauer KD, Duque RE, Shankey TV, editors. Clinical flow cytometry - principals and applications. Baltimore: Williams & Wilkins; 1992.
30. Rabinovitch PS. DNA content histogram and cell cycle analysis. Methods Cell Biol 1994;41:263-296.
31. Rabinovitch PS. Multicycle DNA content and cell cycle analysis software for Windows. User's manual. San Diego: Phoenix Flow Systems, Inc.; 1995.
32. Rueschoff J, Bocker T, Schlegel J, Stumm G, Hofstaedter F. Microsatellite instability: new aspects in the carcinogenesis of colorectal carcinoma. Virch Arch 1995;426:215-222.
33. Sasaki K, Kurose A, Uesugi N, Sugai T. Intratumoral regional heterogeneity of DNA ploidy patterns in colorectal carcinomas. Anal Cell Pathol 1995;8:323-330.
34. Schaafsma HE, Ramaekers FC. Cytokeratin subtyping in normal and neoplastic epithelium, basic principles and diagnostic applications. Pathol Annu 1994;1:21-62.
35. Schutte B, Reynders MMJ, Bosman FT, Blijham GH. Studies with antibromodeoxyuridine antibodies. II. Simultaneous detection of antigen expression and DNA synthesis by in vivo labeling of mouse intestinal mucosa. J Histochem Cytchem 1987;35:371-374.
36. Schutte B, Timmemans MMFJ, Pijpers GFP, Lenders MHJH, Ramaekers FCS. Three parameter flow cytometric analysis for simultaneous detection of cytokeratin, proliferation associated antigens, and DNA content. Cytometry 1995;21:177-186.
37. Shankey TV, Rabinovitch PS, Bagwell B, Bauer KD, Duque RE, Hedley DW, Mayall BH, Wheeless L. Guidelines for implementation of clinical DNA cytometry. Cytometry 1993;14:472-477.
38. Thibodeau SN, French AJ, Roche PC, Cunningham JM, Tester DJ, Lindor NM, Moslem G, Baker SM, Liskay RM, Burgart LJ, Honchel R, Halling KC. Altered expression of hMSH2 and hMLH12 in tumors with microsatellite instability and genetic alteration in mismatch repair genes. Cancer Res 1996;56:4836-4840.
39. van der Linden JC, Herman CJ, Boenders JG, van de Sandt MM, Lindeman J. Flow cytometric DNA content of fresh tumor specimens using keratin-antibody as second stain for two-parameter analysis. Cytometry 1992;13:163-168.
40. Vindelov LL, Christensen IJ. Detergent and proteolytic enzyme-based techniques for nuclear isolation and DNA content analysis. Methods Cell Biol 1994;41:219-229.
41. Visscher DW, Sochacki P, Ottosen S, Wykes S, Crissman JD. Assessment and significance of diploid-range epithelial populations in DNA aneuploid breast carcinomas using multi-parametric flow cytometry. Anal Cell Pathol 1995;8:267-277.
42. Wauters CC, Smedts F, Gerrits LG, Bosman FT, Ramaekers FC. Keratins 7 and 20 as diagnostic markers of carcinomas metastatic to the ovary. Hum Pathol 1995;26:852-855.
43. Wirsching RP, Lamerz R, Wiebecke B, Demmel N, Liewald F, Valet G. Flow cytometric evaluation of colorectal carcinomas as completion of conventional tumor examination. J Exp Clin Cancer Res 1987;6:117-128.
44. Zarbo RJ, Visscher DW, Crissman JD. Two-color multiparametric method for flow cytometric DNA analysis of carcinomas using staining for cytokeratin and leukocyte-common antigen. Anal Quant Cytol Histol 1989;11:391-402.
45. Zarbo RJ, Nakhleh RE, Brown RD, Kubus JJ, Chan KN, Mackowiak P. Prognostic significance of DNA ploidy and proliferation in 309 colorectal carcinomas as determined by two-color multiparametric flow cytometry. Am Cancer Soc 1997;79:2073-2086.