Effects of tryptophan to phenylalanine substitutions on the structure, stability, and enzyme activity of the IIAB(Man) subunit of the mannose transporter of Escherichia coli

Protein Science

Volumn 8, Issue 7, 1999, Pages 1530-1535

  Markovic Housley, Zora ^a   Stolz, Beat ^b   Lanz, Regina ^b   Erni, Bernhard ^b  

^a UNIVERSITY OF BASEL   (Switzerland)

^b UNIVERSITY OF BERN   (Switzerland)

Author keywords

Bacterial phosphotransferase system; Circular dichroism; Fluorescence; Intragenic suppression

Indexed keywords

CARRIER PROTEIN; MANNOSE TRANSPORTER; PHENYLALANINE; PHOSPHOTRANSFERASE; TRYPTOPHAN; UNCLASSIFIED DRUG;

AMINO ACID SUBSTITUTION; ARTICLE; CARBOHYDRATE TRANSPORT; CIRCULAR DICHROISM; CONFORMATIONAL TRANSITION; DIMERIZATION; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ESCHERICHIA COLI; FLUORESCENCE ANALYSIS; HYDROPHILICITY; PRIORITY JOURNAL; PROTEIN FOLDING; PROTEIN PROTEIN INTERACTION; PROTEIN STABILITY; PROTEIN STRUCTURE; STRUCTURE ANALYSIS;

EID: 0032786954     PISSN: 09618368     EISSN: None     Source Type: Journal    
DOI: 10.1110/ps.8.7.1530     Document Type: Article

References (16)

1. Beechem JM, Brand L. 1985. Time-resolved fluorescence of proteins. Annu Rev Biochem 54:43-71.
2. Erni B. 1992. Group translocation of glucose and other carbohydrates by the bacterial phosphotransferase system. Int Rev Cytol 1574:127-148.
3. Man complex of Escherichia coli. J Biol Chem 260:15495-15503.
4. Erni B, Zanolari B, Graff P, Kocher HP. 1989. Mannose permease of E. coli. Domain structure and function of the phosphorylating subunit. J Biol Chem 264:18733-18741.
5. Erni B, Zanolari B, Kocher HP. 1987. The mannose permease of E. coli consists of three different proteins: Amino acid sequence and function in sugar transport, sugar phosphorylation, and penetration of phage lambda DNA. J Biol Chem 262:5238-5247.
6. Gill SC, von Hippel PH. 1989. Calculation of protein extinction coefficients from amino acid sequence data. Anal Biochem 182:319-326.
7. Hochuli M. 1996. The mannose transporter of E. coli: Monitoring conformational changes in the IIAB subunit by fluorescence spectroscopy [Diploma thesis]. Bern, Switzerland: University of Bern.
8. Lee B, Richards FM. 1971. The interpretation of protein structures: Estimation of static accessibility. J Mol Biol 55:379-400.
9. Lengeler JW, Jahreis K, Wehmeier UF. 1994. Enzymes II of the phosphoenolpyruvate-dependent phosphotransferase systems. Their structure and function in carbohydrate transport. Biochim Biophys Acta 1188:1-28.
10. Man of the mannose transporter of E coli. Biochemistry 33:10977-10984.
11. Meadow ND, Fox DK, Roseman S. 1990. The bacterial phosphoenolpyruvate: Glycose phosphotransferase system. Annu Rev Biochem 59:497-542.
12. Nunn RS, Markovic-Housley Z, Génovésio-Taverne JC, Flükiger K, Rizkallah PJ, Jansonius JN, Schirmer T, Erni B. 1996. Structure of the IIA domain of the mannose transporter from E. coli at 1.7 Å resolution. J Mol Biol 259:502-511.
13. Postma PW, Lengeler JW, Jacobson GR. 1996. Phosphoenolpyruvate:carbohydrate phosphotransferase systems. In: Neidhardt FC, Curtiss R, Ingraham JL, Lin ECC, Low KB, Magasanik B, Reznikoff WS, Riley M, Schaechter M, Umbarger HE, eds. Escherichia coli and Salmonella typhimurium: Cellular and molecular biology. Washington, DC: ASM Press, pp 1149-1174.
14. Schauder S, Nunn RS, Lanz R, Erni B, Schirmer T. 1998. Crystal structure of the IIB subunit of the fructose permease from Bacillus subtilis. J Mol Biol 276:591-602.
15. Stanssens P, Opsomer C, McKeown YM, Kramer W, Zabeau M, Fritz HJ. 1989. Efficient oligonucleotide-directed construction of mutations in expression vectors by the gapped duplex DNA method using alternating selectable markers. Nucleic Acids Res 17:4441-4445.
16. Man subunit. J Biol Chem 268:27094-27099.