Use of acidic and basic pH and calcium ion addition in the capillary zone electrophoretic characterization of recombinant human deoxyribonuclease, a complex phosphoglycoprotein

Journal of Chromatography A

Volumn 853, Issue 1-2, 1999, Pages 295-308

  Felten, Chantal ^a   Quan, Cynthia P ^b   Chen, Anthony B ^b   Canova Davis, Eleanor ^b   McNerney, Thomas ^b   Goetzinger, Wolfgang K ^a   Karger, Barry L ^a  

^a NORTHEASTERN UNIVERSITY   (United States)

^b GENENTECH INC   (United States)

Author keywords

Buffer composition; DNAse; Enzymes; Glycoproteins; Phosphoglycoproteins

Indexed keywords

ALKALINE PHOSPHATASE; BUFFER; CALCIUM ION; DORNASE ALFA; GLYCOPROTEIN; PHOSPHOPROTEIN; SIALIDASE;

CALCIUM BINDING; CAPILLARY ZONE ELECTROPHORESIS; CONFERENCE PAPER; CONTROLLED STUDY; ELECTROPHORETIC MOBILITY; ENZYME ANALYSIS; ENZYME LINKED IMMUNOSORBENT ASSAY; GEL ELECTROPHORESIS; ISOELECTRIC FOCUSING; PH; PRIORITY JOURNAL; PROTEIN STRUCTURE;

ANIMALS; CALCIUM; CATTLE; DEOXYRIBONUCLEASES; ELECTROPHORESIS, CAPILLARY; GLYCOPROTEINS; HUMANS; HYDROGEN-ION CONCENTRATION; METALS; MODELS, MOLECULAR; RECOMBINANT PROTEINS;

EID: 0032782494     PISSN: 00219673     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0021-9673(99)00678-0     Document Type: Conference Paper

References (23)

1. Cipolla D., Gonda I., Shire S. J. Pharm. Res. 11:1994;491-498.
2. Davies J., Trindale M., Wallis C., Rosenthal M., Bush A. Pediatr. Pulmonol. Suppl. 16:1997;273-274.
3. Shak S., Capon D.J., Hellmiss R., Marsters S.A., Baker C.L. Proc. Natl. Acad. Sci. USA. 87:1990;9188-9192.
4. Frenz J., Quan C.P., Cacia J., Democko C., Bridenbaugh R., McNerney T. Anal. Chem. 66:1994;335-340.
5. Quan C.P., Cacia J., Frenz J., O'Connor J.V. Pharm. Sci. 3:1997;53-57.
6. Oefner C., Suck D. J. Mol. Biol. 192:1986;605-632.
7. Wolf E., Frenz J., Suck D. Pro. Sci. 4:1995;115. Bio. Chem. Hoppe-Seyler (Spec. Suppl.)376 (1995) S170.
8. Poulos T.L., Price P.A. J. Biol. Chem. 247:1972;2900-2904.
9. Price P.A., Stein W.H., Moore S. J. Biol. Chem. 244:1969;929-932.
10. Chan H.K., Au-Yeung K.L., Gonda I. Pharm. Res. 13:1996;756-761.
11. Price P.A. J. Biol. Chem. 247:1972;2895-2899.
12. Tullis R., Price P.A. J. Biol. Chem. 249:1974;5033-5037.
13. Lizárraga B., Sánchez-Romero D., Gil A., Melgar E. J. Biol. Chem. 253:1978;3191-3195.
14. Thorne J.M., Goetzinger W.K., Chen A.B., Moorhouse K.G., Karger B.L. J. Chromatogr. A. 744:1996;155-165.
15. Klausen N.K., Kornfelt T. J. Chromatogr. A. 718:1995;195-202.
16. Kakehi K., Honda S. J. Chromatogr. A. 720:1996;377-393.
17. Buchacher A., Schulz P., Choromanski J., Schwinn H., Josic D. J. Chromatogr. A. 802:1998;355-366.
18. Bietlot H.P., Girard M. J. Chromatogr. A. 759:1997;177-184.
19. Altria K.D., Bestford J.J. Cap. Electrophoresis. 3:1996;13-23.
20. Pande P.G., Nellore R.V., Bhagat H.R. Anal. Biochem. 204:1992;103-106.
21. W. Goetzinger, B.L. Karger, US Pat., 5 840 388 (1998).
22. Shire S.J. Biochemistry. 22:1983;2664-2671.
23. Shire S.J. Pharm. Biotechnol. 9:1996;393-426.