Synchronization of somatic embryogenesis at high frequency using carrot suspension cultures: Model systems and application in plant development

Methods in Cell Science

Volumn 21, Issue 2-3, 1999, Pages 129-140

  Osuga, Koichi ^a   Masuda, Hiroshi ^b   Komamine, Atsushi ^c  

^a MITSUBISHI HEAVY INDUSTRIES LTD   (Japan)

^b OBIHIRO UNIVERSITY OF AGRICULTURE AND VETERINARY MEDICINE   (Japan)

^c Research Institute of Evolutionary Biology   (Japan)

Author keywords

Artificial seed; Daucus carota; Single cell; Somatic embryogenesis; Synchronization

Indexed keywords

2,4 DICHLOROPHENOXYACETIC ACID; MANNITOL; ZEATIN;

ARTICLE; CARROT; CELL CULTURE; CELL SUSPENSION; CELL SYNCHRONIZATION; DENSITY GRADIENT CENTRIFUGATION; EMBRYO DEVELOPMENT; NONHUMAN; PLANT CELL; PLANT GROWTH;

DAUCUS CAROTA; PLANT GROWTH REGULATORS; PLANT PHYSIOLOGY; SEEDS; TIME FACTORS;

DAUCUS CAROTA; EMBRYOPHYTA;

EID: 0032781967     PISSN: 13815741     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1009884806166     Document Type: Article

References (30)

1. De Vries SC, Booij H, Meyerink P, Huisman G, Wilde HD, Thomas TL, Van Kammen A (1988). Acquisition of embryogenic potential in carrot cell-suspension cultures. Planta 176: 196-204.
2. Fridborg G, Eriksson T (1975). Effects of activated charcoal on growth and morphogenesis in cell cultures. Physiol Plant 34: 306-308.
3. Fridborg G, Pedersén M, Landström L, Eriksson T (1978). The effect of activated charcoal on tissue cultures: Adsorption of metabolites inhibiting morphogenesis. Physiol Plant 43: 104-106.
4. Fujimura T, Komamine A (1975). Effects of various growth regulators on the embryogenesis in a carrot cell suspension culture. Plant Sci Lett 5: 359-364.
5. Fujimura T, Komamine A (1979). Synchronization of somatic embryogenesis in a carrot cell suspension culture. Plant Physiol 64: 162-164.
6. Giuliano G, Rosellini D, Terzi M (1983). A new method for the purification of the different stages of carrot embryoids. Plant Cell Rep 2: 216-218.
7. Goupil P, Hatzopoulos P, Franz G, Hempel FD, You R, Sung ZR (1992). Trascriptional regulation of a seed-specific carrot gene, DC8. Plant Mol Biol 18: 1049-1063.
8. Halperin W (1966). Alternative morphogenetic events in cell suspension. Am J Bot 53: 443-453.
9. Husemann DB, Reinert J (1970). Embryo formation by isolated single cells from tissue cultures of Daucus carota. Protoplasma 70: 49-60
10. Kato H (1968). The serial observations of the adventive embryogenesis in the microculture of carrot tissue. Sci Pap Coll Gen Educ Univ Tokyo 18: 191-197.
11. Lin M, Staba J (1961). Peppermint and spearmint tissue cultures I. Callus formation and submerged culture. Lloydia 24: 139-145.
12. Lo Schiavo FA (1984). Critical review of the procedures for embryo purification. Plant Mol Biol Reporter 2: 15-18.
13. McWilliam AA, Smith SM, Street HE (1974). The origin and development of embryoids in suspension cultures of carrot (Daucus carota). Ann Bot 38: 243-250.
14. Murashige T, Skoog F (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473-497.
15. Nomura K, Komamine A (1985). Identification and isolation of single cells that produce somatic embryos at a high frequency in a carrot suspension culture. Plant Physiol 79: 988-991.
16. Nomura K, Komamine A (1986). Embryogenesis from microinjected single cells in a carrot cell suspension culture. Plant Sci 44: 53-58.
17. Nomura K, Komamine A (1986). Application of microinjection to a high frequency and synchronous somatic embryogenesis system in carrot suspension cultures. Abstract of 6th International Congress of Plant Tissue and Cell Culture: 344.
18. Osuga K, Kamada H, Komamine A (1993). Cell density is an important factor for synchronization of late stages of somatic embryogenesis at high frequency. Plant Tissue Cult Lett 10: 180-183.
19. Osuga K, Kamada H, Komamine A (1993). Density and conditioning factor(s) affect synchronization of somatic embryogenesis in carrot suspension cultures. Abstract of 15th International Botanical Congress, Tokyo: 542.
20. Osuga K, Kamada H, Komamine A (1997). Frequency improvement of somatic embryogenesis at high embryo density by partial replacement of medium in carrot suspension cultures. J Ferment Bioeng 84: 275-278.
21. Osuga K, Komamine A (1994). Synchronization of somatic embryogenesis from carrot cells at high frequency as a basis for the mass production of embryos. Plant Cell Tiss Org Cult 39: 125-135.
22. Reinert J (1958). Morphogenese und ihre Kontrolle an Gewebekulturen aus Karotten. Naturwissenschaften 45: 344-345.
23. Rodriguez DL, Kitto SL, Lomax KM (1990). Mechanical purification of torpedo stage somatic embryos of Daucus carota L. Plant Cell Tiss Org Cult 23: 9-14.
24. Sterk P, Booij H, Schellekens GA, Van Kammen A, De Vries SC (1991). Cell-specific expression of the carrot EP2 lipid transfer protein gene. Plant Cell 3: 907-921.
25. Steward FC, Mapes MO, Hears K (1958). Growth and organized development of cultured cells: II. Organization in cultures grown from freely suspended cells. Am J Bot 45: 705-708.
26. Street HE, Withers LA (1974). The anatomy of embryogenesis in culture. In: Street HE (ed), Tissue culture and plant science, pp 71-100. London: Academic Press.
27. Thomas E, Konar RN, Street H (1972). The fine structure of the embryogenic callus of Ranunculus scleratus L. J Cell Sci 11: 95-109.
28. Warren GS, Fowler MW (1977). A physical method for the separation of various stages in the embryogenesis of carrot cell cultures. Plant Sci Lett 9: 71-76.
29. Wurtele ES, Wang H, Durgerian S, Nikolau BJ, Ulrich TH (1993). Characterization of a gene that is expressed early in somatic embryogenesis of Daucus carota. Plant Physiol 102: 303-312.
30. Yasuda H, Sato T, Masuda H (1998). Rapid and frequent embryogenesis from single cells of regenerated carrot plantlets. Biosci Biotechnol Biochem 62: 1273-1278.