Purification of polysaccharides from a biofilm matrix by selective precipitation of proteins

Biotechnology Techniques

Volumn 13, Issue 6, 1999, Pages 391-393

  Oliveira, R ^a   Marques, F ^a   Azeredo, J ^a  

^a UNIVERSITY OF MINHO   (Portugal)

Author keywords

Differential precipitation; Polysaccharides; Proteins

Indexed keywords

BIOFILM; POLYSACCHARIDE PRODUCTION; PRECIPITATION; PROTEIN; PURIFICATION; TRICHLOROACETIC ACID;

EID: 0032781596     PISSN: 0951208X     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1008954301470     Document Type: Article

References (3)

1. Beri RG, Rollings JE (1995) A technique for the rapid removal of proteins from cell extracts containing polysaccharides. Biotechnol. Tech. 9: 783-786.
2. Doco T, Carcano D, Ramos P, Loones A, Fournet B (1991) Rapid isolation and estimation of polysaccharide from fermented skim milk with Streptococcus salivarius subsp. thermophilus by coupled anion exchange and gel-permeation high-performance liquid chromatography. J. Dairy Res. 5: 147-150.
3. Dubois, M, Gilles, KA, Hamilton, JK, Rebers PA, Smith F (1956). Colorimetric method for determination of sugars and related substances. Anal. Chem. 28: 350-356.