N-acetyi-L-glutamate kinase from Escherichia coli: Cloning of the gene, purification and crystallization of the recombinant enzyme and preliminary X-ray analysis of the free and ligand-bound forms

Acta Crystallographica Section D: Biological Crystallography

Volumn 55, Issue 7, 1999, Pages 1350-1352

  Gil, Fernando ^a   Ramón Maiques, Santiago ^a   Marina, Alberto ^a,b   Fita, Ignacio ^b   Rubio, Vicente ^a  

^a INSTITUTO DE BIOMEDICINA DE VALENCIA   (Spain)

^b INSTITUT DE BIOLOGIA MOLECULAR DE BARCELONA   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

ACETYLGLUTAMATE KINASE; ADENOSINE TRIPHOSPHATE; CARBAMATE KINASE; CLONED GENE; CROSS LINKING; CRYSTAL; CRYSTALLIZATION; ENANTIOMER; ENZYME ACTIVITY; ENZYME PURIFICATION; LIGAND BINDING; MOLECULAR CLONING; N ACETYLGLUTAMIC ACID; PLASMID; RECOMBINANT ENZYME; X RAY DIFFRACTION ANALYSIS;

ACETYLGLUTAMATE KINASE; PHOSPHOTRANSFERASE; PRIMER DNA; RECOMBINANT PROTEIN;

ARTICLE; CHEMISTRY; CRYSTALLIZATION; ENZYMOLOGY; ESCHERICHIA COLI; GENETICS; ISOLATION AND PURIFICATION; MOLECULAR CLONING; NUCLEOTIDE SEQUENCE; PROTEIN CONFORMATION; X RAY CRYSTALLOGRAPHY;

BASE SEQUENCE; CLONING, MOLECULAR; CRYSTALLIZATION; CRYSTALLOGRAPHY, X-RAY; DNA PRIMERS; ESCHERICHIA COLI; PHOSPHOTRANSFERASES (CARBOXYL GROUP ACCEPTOR); PROTEIN CONFORMATION; RECOMBINANT PROTEINS;

EID: 0032777832     PISSN: 09074449     EISSN: None     Source Type: Journal    
DOI: 10.1107/S0907444999005351     Document Type: Article

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