Dissection of the protein G B 1 domain binding site for human IgG Fc fragment

Protein Science

Volumn 8, Issue 8, 1999, Pages 1643-1648

  Sloan, David J ^a   Hellinga, Homme W ^a  

^a DUKE UNIVERSITY MEDICAL CENTER   (United States)

Author keywords

Alanine scanning mutagenesis; B1 domain of protein G; Disassociation constant; Environmentally sensitive fluorophore; Human Fc IgG

Indexed keywords

ALANINE; GLUTAMINE; IMMUNOGLOBULIN FC FRAGMENT; PROTEIN G;

ARTICLE; BINDING SITE; COMPLEX FORMATION; DISSOCIATION CONSTANT; ENTHALPY; HUMAN; HYDROGEN BOND; MUTAGENESIS; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN INTERACTION;

EID: 0032777649     PISSN: 09618368     EISSN: None     Source Type: Journal    
DOI: 10.1110/ps.8.8.1643     Document Type: Article

References (32)

1. Argos P. 1988. An investigation of protein subunit and domain interfaces. Protein Eng 2:101-113.
2. Armstrong N, Adey NB, McConnell SJ, Kay BK. 1996. Phage display of peptides and proteins. In: Kay BK, Winter J, McCafferty J, eds. San Diego, CA: Academic Press, Inc. pp 35-53.
3. Bogan AA, Thorn KS. 1998. Anatomy of hot spots in protein interfaces. J Mol Biol 280:1-9.
4. Boyle MDP. 1990. Bacterial immunoglobulin-binding proteins. San Diego, CA: Academic Press.
5. Crick FHC. 1952. Is α-keratin a coiled coil? Nature 170:882-883.
6. Crick FHC. 1953. The packing of α-helices: Simple coiled-coils. Acta Crystallogr 6:689-697.
7. Davies DR, Cohen GH. 1996. Interactions of protein antigens with antibodies. Proc Natl Acad Sci USA 93:7-12.
8. Deisenhofer J. 1981. Crystallographic refinement and atomic models of a human Fc fragment and its complex with fragment B of protein A from Staphylococcus aureus at 2.9 and 2.8 Å resolution. Biochemistry 20:2361-2370.
9. Derrick JP, Wigley DB. 1992. Crystal structure of a streptococcal protein G domain bound to an Fab fragment. Nature 359:752-754.
10. Fierke CA, Calderone TL, Krebs JF. 1991. Functional consequences of engineering the hydrophobic pocket of carbonic anhydrase II. Biochemistry 30:11054-11063.
11. Frick I-M, Wikström M, Forsén S, Drakenberg T, Gomi H, Sjöbring U, Björck L. 1992. Convergent evolution among immunoglobulin G-binding bacterial proteins. Proc Natl Acad Sci USA 89:8532-8536.
12. Goward CR, Scawen MD, Murphy JP. 1993. Molecular evolution of bacterial cell-surface proteins. Trends Biochem Sci 18:136-140.
13. Gronenborn AM, Clore GM. 1993. Identification of the contact surface of a Streptococcal protein G domain complexed with a human Fc fragment. J Mol Biol 233:331-335.
14. Ho SN, Hunt HD, Horton RM, Pullen JK, Pease LR. 1989. Site-directed mutagenesis by overlap extension using the polymerase chain reaction. Gene 77:55-59.
15. Hochuli E, Bannwarth W, Döbeli H, Gentz R, Stuber D. 1988. Genetic approach to facilitate purification of recombinant proteins with a novel metal chelate absorbent. Biotechnology 6:1321-1325.
16. Jones S, Thornton JM. 1996. Principles of protein-protein interactions. Proc Natl Acad Sci USA 93:13-20.
17. Kato K, Lian L-Y, Barsukov IL, Derrick JP, Kim H, Tanaka R, Yoshino A, Shiraishi M, Shimada I, Arata Y, Roberts GCK. 1995. Model for the complex between protein G and an antibody Fc fragment in solution. Structure 3:79-85.
18. Kunkel TA. 1985. Rapid and site-specific mutagenesis without phenotypic selection. Proc Natl Acad Sci USA 82:488-492.
19. LoConte L, Chothia C, Janin J. 1999. The atomic structure of protein-protein recognition sites. J Mol Biol 285:2177-2198.
20. McConnell SJ, Kendell ML, Reilly TM, Hoess RH. 1994. Constrained libraries as a tool for finding mimotopes. Gene 151:115-118.
21. Messing J. 1991. Cloning in M13 phage or how to use biology at its best. Gene 100:3-12.
22. Richards FM. 1977. Areas, volumes, packing, and protein structures. Anna Rev Biophys Bioeng 6:151-176.
23. Sauer-Eriksson AE, Kleywegt GJ, Uhlén M, Jones TA. 1995. Crystal structure of the C2 fragment of streptococcal protein G in complex with the Fc domain of human IgG. Structure 3:265-278.
24. Segel IH. 1975. Enzyme kinetics. New York: John Wiley & Sons.
25. Sloan DJ, Hellinga HW. 1998. Structure-based engineering of environmentally sensitive fluorophores for monitoring protein-protein interactions. Protein Eng 11:819-823.
26. Smith GP, Scott JK. 1993. Libraries of peptides and proteins displayed on filamentous phage. Methods Enzymol 217:228-257.
27. Ståhl S, Nygren P-Å, Sjölander A, Uhlén M. 1993. Engineered bacterial receptors in immunology. Curr Opin Immunol 5:272-277.
28. Stites WE. 1997. Protein-protein interactions: Interface structure, binding thermo-dynamics, and mutational analysis. Chem Rev 97:1233-1250.
29. Stone GC, Sjöbrin U, Björck L, Sjöquist J, Barber CV, Nardella FA. 1989. The Fc binding site for streptococcal protein G is in the Cγ2-Cγ3 interface region of IgG and is related to the sites that bind Staphylococcal protein A and human rheumatoid factors. J Immunol 143:565-510.
30. Tsunogae Y, Tanaka I, Yamane T, Kikkawa J, Ashida T, Ishikawa C, Watanabe K, Nakamura S, Takahashi K. 1986. Structure of the trypsin-binding domain of Bowman-Birk type protease inhibitor and its interaction with trypsin. J Biochem 100:1637-1646.
31. Wells JA. 1991. Systematic mutational analyses of protein-protein interfaces. Methods Enzymol 202:390-411.
32. Wells JA, deVos AM. 1996. Hematopoietic receptor complexes. Ann Rev Biochem 65:609-634.