RNA content differs in slow and fast muscle fibers: Implications for interpretation of changes in muscle gene expression

Journal of Histochemistry and Cytochemistry

Volumn 47, Issue 8, 1999, Pages 995-1004

  Habets, Petra E M H ^a,b   Franco, Diego ^a   Ruijter, Jan M ^a   Sargeant, Anthony J ^b,c   Sant'Ana Pereira, José A A ^a,d   Moorman, Antoon F M ^a,e  

^a UNIVERSITY OF AMSTERDAM   (Netherlands)

^b MANCHESTER METROPOLITAN UNIVERSITY   (United Kingdom)

^c VU UNIVERSITY AMSTERDAM   (Netherlands)

^d UNIVERSITY OF WISCONSIN SCHOOL OF MEDICINE AND PUBLIC HEALTH   (United States)

^e ACADEMIC MEDICAL CENTER   (Netherlands)

Author keywords

Densitometry; In situ hybridization; RNA content; Single fibers; Skeletal muscle

Indexed keywords

RNA;

ANIMAL TISSUE; ARTICLE; CELLULAR DISTRIBUTION; DENSITOMETRY; FAST MUSCLE FIBER; GENE EXPRESSION REGULATION; IN SITU HYBRIDIZATION; MUSCLE DEVELOPMENT; NONHUMAN; NORTHERN BLOTTING; PRIORITY JOURNAL; RAT; RNA ANALYSIS; SLOW MUSCLE FIBER;

ANIMALIA; EUKARYOTA;

EID: 0032777201     PISSN: 00221554     EISSN: None     Source Type: Journal    
DOI: 10.1177/002215549904700803     Document Type: Article

References (43)

1. Aigner S, Pette D (1990) In situ hybridisation of slow myosin heavy chain mRNA in normal and transforming rabbit muscles with the use of non-radioactively labeled cRNA. Histochemistry 95:11-18
2. Allen DL, Yasui W, Tanaka T, Ohira Y, Nagaoka S, Sekiguchi C, Hinds WE, Roy RR, Edgerton VR (1996) Myonuclear number and myosin heavy chain expression in rat soleus single muscle fibres after spaceflight. J Appl Physiol 81:145-151
3. Boheler KR, Chassagne C, Martix X, Wisnewsky C, Schwartz K (1992) Cardiac expression of a and β myosin heavy chains and sarcomeric a actins are regulated through transcriptional mechanisms. J Biol Chem 267:12979-12985
4. Caiozzo VJ, Haddad F, Baker MJ, Baldwin KM (1996) Influence of mechanical loading on myosin heavy chain protein and mRNA expression. J Appl Physiol 80:1503-1512
5. Caiozzo VJ, Ma E, McCue SA, Smith E, Herrick RE, Baldwin KM (1992) A new model for modulating myosin isoform expression by altered mechanical activity. J Appl Physiol 73:1432-1440
6. Chirgwin JM, Przybyla AE, MacDonald J, Ruiter WJ (1979) Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry 18:5294-5299
7. Chomczynski P, Sacchi N (1987) Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 162:156-159
8. Choo JJ, Horan MA, Little RA, Rothwell NJ (1992) Anabolic effects of clenbuterol on skeletal muscle are mediated by beta 2-adrenoceptor activation. Am J Physiol 263:E50-56
9. Conover WJ (1980) Practical Nonparametric Statistics. 2nd ed. New York, Wiley
10. De Nardi C, Ausoni S, Moretti P, Gorza L, Velleca M, Buckingham M, Schiaffino S (1993) Type IIX myosin heavy chain is coded by a muscle fibre type-specific and developmentally regulated gene. J Cell Biol 123:823-835
11. De Ruiter CJ, de Haan A, Sargeant AJ (1995) Physiological characteristics of two extreme muscle compartments in gastrocnemius of the anaesthetized rat. Acta Physiol Scand 153:313-324
12. De Ruiter CJ, Habets PEMH, de Haan A, Sargeant AJ (1996) In vivo IIX and IIB fiber recruitment in gastrocnemius muscle of the rat is compartment related. J Appl Physiol 81:933-942
13. Dix DJ, Eisenberg BR (1988) In situ hybridisation and immunocytohistochemistry in serial sections of rabbit skeletal muscle to detect myosin expression. J Histochem Cytochem 36:1519-1526
14. Flaim K, Copenhower ME, Jefferson LS (1980) Effects of diabetes on protein synthesis in fast- and slow-twitch rat skeletal muscle. Am J Physiol 239:E88-95
15. Goldberg AL (1967) Protein synthesis in tonic and phasic skeletal muscles. Nature 216:1219-1220
16. Hesketh J, Campbell G, Loveridge N (1991) Myosin heavy-chain mRNA is present in both myofibrillar and subsarcolemmal regions of muscle fibres. Biochem J 279:309-310
17. Hood DA, Simoneau J (1989) Rapid isolation of total RNA from small mammal and human skeletal muscle. Am J Physiol 256:1029-1096
18. Hood DA, Terjung RL (1987) Leucine metabolism in perfused rat skeletal muscle fibres during contractions. Am J Physiol 253:E636-647
19. Horne Z, Hesketh J (1990) Immunological localisation of ribosomes in striated rat muscle. Biochem J 268:231-236
20. Jaenicke T, Diederich KW, Haas W, Schleich J, Lichter P, Pfordt M, Bach A, Vosberg HP (1990) The complete sequence of the human beta-myosin heavy chain gene and a comparative analysis of its product. Genomics 8:194-206
21. Jonker A, de Boer PAJ, van den Hoff MJB, Lamers WH, Moorman AFM (1997) Towards quantitative in situ hybridization. J Histochem Cytochem 45:413-423
22. Kurowski TT, Zak R, Hickson R (1987) Development of glucocorticoid-induced growth and atrophy in heart and skeletal muscle. J Cell Biol 105:11a
23. Mahdavi V, Izumo S, Nadal-Ginard B (1987) Developmental and hormonal regulation of sarcomeric myosin heavy chain gene family. Circ Res 60:804-814
24. Melton DA, Krieg PA, Rebagliati MR, Maniatis T, Zinn K, Green MR (1984) Efficient in vitro synthesis of biologically active RNA and RNA hybridisation probes from plasmids containing a bacreriophage SP6 promoter. Nucleic Acids Res 12:7035-7056
25. Millward DJ, Garlick PJ, James WPT, Nnanyelugo DO, Ryatt JS (1973) Relationship between protein synthesis and RNA content in skeletal muscle, Nature 241:204-205
26. Moorman AFM, de Boer PAJ, Vermeulen JLM, Lamers WH (1993) Practical aspects of radio-isotopic in situ hybridization on RNA. Histochem J 25:251-266
27. Moss RL, Diffee GM, Graeser ML (1995) Contractile properties of skeletal muscle fibres in relation to myofibrillar protein isoforms. Rev Physiol Biochem Pharmacol 126:1-63
28. Munro NH (1966) The determination of nucleic acids. Methods Biochem Anal 14:113-176
29. Pette D, Staron RS (1990) Cellular and molecular diversities of mammalian skeletal muscle fibres. Rev Physiol Biochem Pharmacol 116:1-76
30. Sambrook J, Fritsch JE, Maniatis T (1989) Molecular Cloning: A Laboratory Manual. Cold Spring Harbor, NY, Cold Spring Harbor Laboratory
31. Sant'Ana Pereira JAA, de Haan A, Wessels A, Moorman AFM, Sargeant AJ (1995b) The mATPase histochemical profile of rat type IIX fibres: correlation with MyHC immunolabelling. Histochem J 27:715-722
32. Sant'Ana Pereira JAA, Ennion S, Sargeant AJ, Moorman AFM, Goldspink G (1997) Comparison of the molecular, antigenic and ATPase determinants of fast MyHCs in rat and humans: a single fibre study. Pflugers Arch Eur J Physiol 435:151-163
33. Sant'Ana Pereira JAA, Wessels A, Nytmans L, Moorman AFM, Sargeant AJ (1995a) New method for the accurate characterisation of single human fibres demonstrates a relation between mATPase and MyHC expression in pure and hybrid fibre types. J Muscle Res Cell Motil 16:21-34
34. Schiaffino S, Gorza L, Sartore S, Saggin L, Ausoni S, Vianello M, Gundersen K, Lomo T (1989b) Three myosin heavy chain isoforms in Type 2 skeletal muscle fibres. J Muscle Res Cell Motil 10:197-205
35. Schiaffino S, Reggiani C (1994) Myosin isoforms in mammalian skeletal muscle. J Appl Physiol 77:493-501
36. Schiaffino S, Reggiani C (1996) Molecular diversity of myofibrillar proteins: gene regulation and functional significance. Physiol Rev 76:371-423
37. Schiaffino S, Samuel JL, Sassoon D, Lompré AM, Garner I, Marotte F, Buckingham M, Rappaport L, Schwartz K (1989a) Nonsynchronous accumulation of α-skeletal actin and β-myosin heavy chain mRNAs during early stages of pressure-overload-induced cardiac hypertrophy demonstrated by in situ hybridization. Circ Res 64:937-948
38. Schmalbruch H, Hellhammer U (1977) The number of nuclei in adult rat muscles with special reference to satellite cells. Anat Rec 189:169-176
39. Steffen JM, Musacchia XJ (1984) Effect of hypokinesia and hypodynamia on protein, RNA and DNA in rat hindlimb muscles. Am J Physiol 247:R728-732
40. Swyngedauw B (1980) Developmental and functional adaptations of contractile proteins in cardiac and skeletal muscles. Physiol Rev 66:710-771
41. Tas J, James J (1981) Quantitative cytochemistry of nuclear and cytoplasmic protein using the naphthol Yellow S and dinitrofluorobenzene staining methods. Histochem J 13:711-716
42. Tseng BS, Kasper CE, Edgerton VR (1994) Cytoplasm-to-myonucleus ratios and succinate dehydrogenase activities in adult rat slow and fast muscle fibres. Cell Tissue Res 275:39-49
43. van den Hoff MJB, Lekanne Deprez RH, Monteiro M, de Boer PAJ, Charles R, Moorman AFM (1997) Developmental changes in rat cardiac DNA, RNA and protein tissue base: implications for the interpretations of changes in gene expression. J Mol Cell Cardiol 29:629-639